Expression of hypoxia‑inducible factor‑1α, endothelin‑1 and adrenomedullin in newborn rats with hypoxia‑induced pulmonary hypertension

Wang,Le; Zhou,Ying; Li,Mingxia; Zhu,Yanping

doi:10.3892/etm.2014.1728

Expression of hypoxia‑inducible factor‑1α, endothelin‑1 and adrenomedullin in newborn rats with hypoxia‑induced pulmonary hypertension

Authors:
- Le Wang
- Ying Zhou
- Mingxia Li
- Yanping Zhu
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Affiliations: Department of Neonatology, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China
Published online on: May 20, 2014 https://doi.org/10.3892/etm.2014.1728
Pages: 335-339

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Abstract

Hypoxia‑inducible factor (HIF)‑1α is associated with hypoxia‑induced pulmonary hypertension (HPH) in adults. In the present study, the expression levels of HIF‑1α, endothelin (ET)‑1 and adrenomedullin (ADM) were analyzed during HPH in neonates. In total, 96 newborn rats were subjected to hypoxia or normoxia for 3, 5, 7, 10, 14 or 21 days (n=8 per subgroup). HIF‑1α, ET‑1 and ADM expression levels were measured by quantitative polymerase chain reaction. In addition, the intima‑media thickness/external diameter ratio (MT%) and medial wall cross‑sectional area/vessel total cross‑sectional area ratio (MA%) were calculated to evaluate pulmonary vascular remodeling. The mean pulmonary arterial pressure (mPAP) increased with exposure to hypoxia. Furthermore, the expression levels of HIF‑1α, ET‑1 and ADM in the lungs were shown to increase after three and five days of hypoxia, while the MT% and MA% increased after seven days of hypoxia, as compared with the controls (P<0.05). Therefore, the expression of HIF‑1α, ET‑1 and ADM is upregulated in the lungs of newborn rats during early HPH. At later stages, the mPAP increases, vascular remodeling occurs and HIF‑1α, ET‑1 and ADM expression levels restore to normal levels.

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