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Article

TNF‑α enhances the effect of TGF‑β on Gli2 expression in the KG‑1 leukemic cell line

  • Authors:
    • Zhe Li
    • Bin Li
    • Jing Pan
    • Jieping Jin
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, First Affiliated Hospital of Liaoning Medical College, Jinzhou, Liaoning 121001, P.R.China
  • Pages: 676-680
    |
    Published online on: May 28, 2014
       https://doi.org/10.3892/etm.2014.1743
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Abstract

The Hedgehog (Hh) signaling pathway regulates a variety of tumor‑related diseases, including leukemia. The present study aimed to determine whether there was an interaction between the Hh signaling pathway and transforming growth factor (TGF)‑β in the KG‑1 cell line. KG‑1 cells were treated with TGF‑β, tumor necrosis factor (TNF)‑α and specific inhibitor of smad3 (SIS3). The expression level of Gli family zinc finger 2 (Gli2) was detected by quantitative polymerase chain reaction (qPCR) and western blot analyses. The results revealed that TGF‑β significantly decreased the expression level of Gli2 in KG‑1 cells, and that TNF‑α and TGF‑β together further reduced Gli2 expression in KG‑1 cells. SIS3 inhibited the effect of TGF‑β. These results suggest that Gli2 expression in KG‑1 cells is suppressed by TGF‑β in a Smad3‑dependent manner, TNF‑α can enhance the effect of TGF‑β on Gli2 expression and that this occurs independently of Hh receptor signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Li Z, Li B, Pan J and Jin J: TNF‑α enhances the effect of TGF‑β on Gli2 expression in the KG‑1 leukemic cell line. Exp Ther Med 8: 676-680, 2014.
APA
Li, Z., Li, B., Pan, J., & Jin, J. (2014). TNF‑α enhances the effect of TGF‑β on Gli2 expression in the KG‑1 leukemic cell line. Experimental and Therapeutic Medicine, 8, 676-680. https://doi.org/10.3892/etm.2014.1743
MLA
Li, Z., Li, B., Pan, J., Jin, J."TNF‑α enhances the effect of TGF‑β on Gli2 expression in the KG‑1 leukemic cell line". Experimental and Therapeutic Medicine 8.2 (2014): 676-680.
Chicago
Li, Z., Li, B., Pan, J., Jin, J."TNF‑α enhances the effect of TGF‑β on Gli2 expression in the KG‑1 leukemic cell line". Experimental and Therapeutic Medicine 8, no. 2 (2014): 676-680. https://doi.org/10.3892/etm.2014.1743
Copy and paste a formatted citation
x
Spandidos Publications style
Li Z, Li B, Pan J and Jin J: TNF‑α enhances the effect of TGF‑β on Gli2 expression in the KG‑1 leukemic cell line. Exp Ther Med 8: 676-680, 2014.
APA
Li, Z., Li, B., Pan, J., & Jin, J. (2014). TNF‑α enhances the effect of TGF‑β on Gli2 expression in the KG‑1 leukemic cell line. Experimental and Therapeutic Medicine, 8, 676-680. https://doi.org/10.3892/etm.2014.1743
MLA
Li, Z., Li, B., Pan, J., Jin, J."TNF‑α enhances the effect of TGF‑β on Gli2 expression in the KG‑1 leukemic cell line". Experimental and Therapeutic Medicine 8.2 (2014): 676-680.
Chicago
Li, Z., Li, B., Pan, J., Jin, J."TNF‑α enhances the effect of TGF‑β on Gli2 expression in the KG‑1 leukemic cell line". Experimental and Therapeutic Medicine 8, no. 2 (2014): 676-680. https://doi.org/10.3892/etm.2014.1743
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