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Article

Mitochondrial DNA mutation screening of male patients with obstructive sleep apnea‑hypopnea syndrome

  • Authors:
    • Xiao‑Ying Huang
    • Hong Li
    • Xiao‑Mei Xu
    • Liang‑Xing Wang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325003, P.R. China, Department of Respiratory Disease, Hospital of Huabei Petroleum Administration Bureau, Renqiu, Hebei 062552, P.R. China, Department of Respiratory Disease, Hospital of Huabei Petroleum Administration Bureau, Renqiu, Hebei 062552, P.R. China
  • Pages: 519-524
    |
    Published online on: May 29, 2014
       https://doi.org/10.3892/etm.2014.1748
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Abstract

The aim of the present study was to analyze the differences between the genes of the mitochondrial DNA (mtDNA) displacement loop (D‑loop) region and the Cambridge Reference sequence, in order to screen the mutation sites and investigate the correlation between mutations, clinical parameters and complications associated with obstructive sleep apnea‑hypopnea syndrome (OSAHS). mtDNA was obtained from male patients with OSAHS in the Zhejiang Province. In total, 60 male patients with OSAHS and 102 healthy adults were assessed to determine the levels of fasting blood glucose, total cholesterol, triglyceride (TG) and high‑density and low‑density lipoproteins (LDL). Furthermore, peripheral mtDNA was extracted and bidirectional sequencing was conducted to enable mutation screening. In the mtDNA D‑loop region, 178 mutation sites were identified, of which 115 sites were present in the two groups. The number of non‑common sites in the OSAHS group was significantly higher compared with the control group (P<0.05). No statistically significant difference was observed in the mutations among the mild, moderate and severe OSAHS groups (P>0.05). A total of 21 cases in the severe OSAHS group exhibited mutation rates of >10%. In the control group, there were 24 cases where the np73A‑G and np263A‑G mutations were predominant. The np303‑np315 region was identified to be the highly variable region and various mutation forms were observed. Statistically significant differences were observed in the neck perimeter, TG and LDL levels among the OSAHS‑no‑mutation subgroups (P<0.05) and LDL was shown to be associated with an mtDNA mutation in the OSAHS group. Numerous polymorphic mutation sites were identified in the mtDNA D‑loop region of the OSAHS group. Therefore, mtDNA mutation sites may be closely associated with the clinical manifestations and complications of OSAHS.
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Copy and paste a formatted citation
Spandidos Publications style
Huang XY, Li H, Xu XM and Wang LX: Mitochondrial DNA mutation screening of male patients with obstructive sleep apnea‑hypopnea syndrome. Exp Ther Med 8: 519-524, 2014.
APA
Huang, X., Li, H., Xu, X., & Wang, L. (2014). Mitochondrial DNA mutation screening of male patients with obstructive sleep apnea‑hypopnea syndrome. Experimental and Therapeutic Medicine, 8, 519-524. https://doi.org/10.3892/etm.2014.1748
MLA
Huang, X., Li, H., Xu, X., Wang, L."Mitochondrial DNA mutation screening of male patients with obstructive sleep apnea‑hypopnea syndrome". Experimental and Therapeutic Medicine 8.2 (2014): 519-524.
Chicago
Huang, X., Li, H., Xu, X., Wang, L."Mitochondrial DNA mutation screening of male patients with obstructive sleep apnea‑hypopnea syndrome". Experimental and Therapeutic Medicine 8, no. 2 (2014): 519-524. https://doi.org/10.3892/etm.2014.1748
Copy and paste a formatted citation
x
Spandidos Publications style
Huang XY, Li H, Xu XM and Wang LX: Mitochondrial DNA mutation screening of male patients with obstructive sleep apnea‑hypopnea syndrome. Exp Ther Med 8: 519-524, 2014.
APA
Huang, X., Li, H., Xu, X., & Wang, L. (2014). Mitochondrial DNA mutation screening of male patients with obstructive sleep apnea‑hypopnea syndrome. Experimental and Therapeutic Medicine, 8, 519-524. https://doi.org/10.3892/etm.2014.1748
MLA
Huang, X., Li, H., Xu, X., Wang, L."Mitochondrial DNA mutation screening of male patients with obstructive sleep apnea‑hypopnea syndrome". Experimental and Therapeutic Medicine 8.2 (2014): 519-524.
Chicago
Huang, X., Li, H., Xu, X., Wang, L."Mitochondrial DNA mutation screening of male patients with obstructive sleep apnea‑hypopnea syndrome". Experimental and Therapeutic Medicine 8, no. 2 (2014): 519-524. https://doi.org/10.3892/etm.2014.1748
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