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August-2014 Volume 8 Issue 2

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma

  • Authors:
    • Yang Yang
    • Lv Hui
    • Che Yuqin
    • Li Jie
    • Hou Shuai
    • Zhou Tiezhu
    • Wang Wei
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China
  • Pages: 563-566
    |
    Published online on: June 4, 2014
       https://doi.org/10.3892/etm.2014.1756
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Abstract

Saw palmetto extract can induce the apoptosis of prostate cancer cells. The aim of the present study was to investigate the effect of saw palmetto extract on the phosphatidylinositol 3‑kinase (PI3K)/Akt signaling transduction pathway in human glioma U87 and U251 cell lines. Suspensions of U87 and U251 cells in a logarithmic growth phase were seeded into six‑well plates at a density of 104 cells/well. In the experimental group, 1 µl/ml saw palmetto extract was added, while the control group was cultured without a drug for 24 h. The expression levels of PI3K, B‑cell lymphoma‑extra large (Bcl‑xL) and p53 were evaluated through western blot analysis. In the experimental group, the U87 and U251 cells exhibited a lower expression level of PI3K protein as compared with the control group (t=6.849; P<0.001). In addition, the two cell lines had a higher expression level of p53 protein in the experimental group as compared with the control group (t=40.810; P<0.001). Protein expression levels of Bcl‑xL decreased significantly in the experimental group as compared with the control group (t=19.640; P=0.000). Therefore, saw palmetto extract induces glioma cell growth arrest and apoptosis via decreasing PI3K/Akt signal transduction.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Hui L, Yuqin C, Jie L, Shuai H, Tiezhu Z and Wei W: Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma. Exp Ther Med 8: 563-566, 2014.
APA
Yang, Y., Hui, L., Yuqin, C., Jie, L., Shuai, H., Tiezhu, Z., & Wei, W. (2014). Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma. Experimental and Therapeutic Medicine, 8, 563-566. https://doi.org/10.3892/etm.2014.1756
MLA
Yang, Y., Hui, L., Yuqin, C., Jie, L., Shuai, H., Tiezhu, Z., Wei, W."Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma". Experimental and Therapeutic Medicine 8.2 (2014): 563-566.
Chicago
Yang, Y., Hui, L., Yuqin, C., Jie, L., Shuai, H., Tiezhu, Z., Wei, W."Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma". Experimental and Therapeutic Medicine 8, no. 2 (2014): 563-566. https://doi.org/10.3892/etm.2014.1756
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Hui L, Yuqin C, Jie L, Shuai H, Tiezhu Z and Wei W: Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma. Exp Ther Med 8: 563-566, 2014.
APA
Yang, Y., Hui, L., Yuqin, C., Jie, L., Shuai, H., Tiezhu, Z., & Wei, W. (2014). Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma. Experimental and Therapeutic Medicine, 8, 563-566. https://doi.org/10.3892/etm.2014.1756
MLA
Yang, Y., Hui, L., Yuqin, C., Jie, L., Shuai, H., Tiezhu, Z., Wei, W."Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma". Experimental and Therapeutic Medicine 8.2 (2014): 563-566.
Chicago
Yang, Y., Hui, L., Yuqin, C., Jie, L., Shuai, H., Tiezhu, Z., Wei, W."Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma". Experimental and Therapeutic Medicine 8, no. 2 (2014): 563-566. https://doi.org/10.3892/etm.2014.1756
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