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Article

Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes

  • Authors:
    • Jia Cheng
    • Linlin Tang
    • Qingxiao Hong
    • Huadan Ye
    • Xuting Xu
    • Leiting  Xu
    • Shizhong Bu
    • Qinwen Wang
    • Dongjun Dai
    • Danjie Jiang
    • Shiwei Duan
  • View Affiliations / Copyright

    Affiliations: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China
  • Pages: 579-584
    |
    Published online on: June 6, 2014
       https://doi.org/10.3892/etm.2014.1766
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Abstract

Promoter DNA methylation may reflect the interaction between genetic backgrounds and environmental factors in the development of metabolic disorders, including type 2 diabetes (T2D). Calcium/calmodulin‑dependent protein kinase 1D (CAMK1D), cryptochrome 2 (CRY2) and calmodulin 2 (CALM2) genes have been identified to be associated with a risk of T2D. Therefore, the aim of the present study was to investigate the contribution of promoter DNA methylation of these genes to the risk of T2D. Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age‑ and gender‑matched healthy controls. The results demonstrated that the promoters of these three genes were hypomethylated in the peripheral blood of all the subjects, and DNA methylation of these three genes did not contribute to the risk of T2D.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng J, Tang L, Hong Q, Ye H, Xu X, Xu L, Bu S, Wang Q, Dai D, Jiang D, Jiang D, et al: Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes. Exp Ther Med 8: 579-584, 2014.
APA
Cheng, J., Tang, L., Hong, Q., Ye, H., Xu, X., Xu, L. ... Duan, S. (2014). Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes. Experimental and Therapeutic Medicine, 8, 579-584. https://doi.org/10.3892/etm.2014.1766
MLA
Cheng, J., Tang, L., Hong, Q., Ye, H., Xu, X., Xu, L., Bu, S., Wang, Q., Dai, D., Jiang, D., Duan, S."Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes". Experimental and Therapeutic Medicine 8.2 (2014): 579-584.
Chicago
Cheng, J., Tang, L., Hong, Q., Ye, H., Xu, X., Xu, L., Bu, S., Wang, Q., Dai, D., Jiang, D., Duan, S."Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes". Experimental and Therapeutic Medicine 8, no. 2 (2014): 579-584. https://doi.org/10.3892/etm.2014.1766
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng J, Tang L, Hong Q, Ye H, Xu X, Xu L, Bu S, Wang Q, Dai D, Jiang D, Jiang D, et al: Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes. Exp Ther Med 8: 579-584, 2014.
APA
Cheng, J., Tang, L., Hong, Q., Ye, H., Xu, X., Xu, L. ... Duan, S. (2014). Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes. Experimental and Therapeutic Medicine, 8, 579-584. https://doi.org/10.3892/etm.2014.1766
MLA
Cheng, J., Tang, L., Hong, Q., Ye, H., Xu, X., Xu, L., Bu, S., Wang, Q., Dai, D., Jiang, D., Duan, S."Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes". Experimental and Therapeutic Medicine 8.2 (2014): 579-584.
Chicago
Cheng, J., Tang, L., Hong, Q., Ye, H., Xu, X., Xu, L., Bu, S., Wang, Q., Dai, D., Jiang, D., Duan, S."Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes". Experimental and Therapeutic Medicine 8, no. 2 (2014): 579-584. https://doi.org/10.3892/etm.2014.1766
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