Construction of tissue‑engineered bone using a bioreactor and platelet‑rich plasma

Wang,Dong; Jiang,Honglei; Wang,Shuzhen; Li,Huibo; Zhang,Huawu; Zhao,Lei; Peng,Tao; Cao,Zhong; Sun,Shui

doi:10.3892/etm.2014.1774

August-2014 Volume 8 Issue 2

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August-2014 Volume 8 Issue 2

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Article

Construction of tissue‑engineered bone using a bioreactor and platelet‑rich plasma

Authors:
- Dong Wang
- Honglei Jiang
- Shuzhen Wang
- Huibo Li
- Huawu Zhang
- Lei Zhao
- Tao Peng
- Zhong Cao
- Shui Sun
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Affiliations: Department of Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China, Department of Cardiology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China, Department of Equipment, The People's Hospital of Laiwu, Laiwu, Shandong 271100, P.R. China
Pages: 413-418
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Published online on: June 11, 2014

https://doi.org/10.3892/etm.2014.1774
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Abstract

The aim of the present study was to construct tissue‑engineered bone using a bioreactor and platelet‑rich plasma (PRP). Bone marrow mesenchymal stem cells (BMSCs) and β‑tricalcium phosphate (β‑TCP) were cultured in a perfusion bioreactor with PRP‑containing medium for 21 days to form a BMSC‑TCP composite. Rabbits were then implanted with the BMSC‑TCP composite. The morphology of the implanted BMSC‑TCP composite was observed three months after surgery by scanning electron microscopy and hematoxylin and eosin (H&E) staining. In addition, the expression of cluster of differentiation (CD)31 and von Willebrand factor (WF) in the implanted BMSC‑TCP composite was detected using immunohistochemistry. Bone formation was determined by comprehensive testing Following culture in a perfusion bioreactor and PRP, the BMSCs adhered to the β‑TCP scaffold and the secretion of extracellular matrix was observed. The spreading and proliferation of cells was found to be enhanced on the scaffold. Furthermore, the vascular endothelial cell markers CD31 and VEF, were positively expressed. Therefore, these results suggest that tissue‑engineered bone may be constructed using a bioreactor and PRP. PRP, which contains multiple growth factors, may promote vascularization of tissue‑engineered bone.

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1	Song HJ, Lan BS, Cheng B, et al: Treatment of early avascular necrosis of femoral head by small intestinal submucosal matrix with peripheral blood stem cells. Transplant Proc. 43:2027–2032. 2011. View Article : Google Scholar : PubMed/NCBI
2	Feng L, Wu H, EL, et al: Effects of vascular endothelial growth factor 165 on bone tissue engineering. PLoS One. 8:e829452013. View Article : Google Scholar : PubMed/NCBI
3	Zou D, Zhang Z, Ye D, et al: Repair of critical-sized rat calvarial defects using genetically engineered bone marrow-derived mesenchymal stem cells overexpressing hypoxia-inducible factor-1α. Stem Cells. 29:1380–1390. 2011.PubMed/NCBI
4	Marques LF, Stessuk T, Camargo IC, Sabeh N Junior, Santos LD and Ribeiro-Paes JT: Platelet-rich plasma (PRP): Methodological aspects and clinical applications. Platelets. Feb 10–2014.(Epub ahead of print).
5	Kim TH, Kim SH, Sándor GK and Kim YD: Comparison of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) in rabbit-skull defect healing. Arch Oral Biol. 59:550–558. 2014. View Article : Google Scholar : PubMed/NCBI
6	Lin BN, Whu SW, Chen CH, et al: Bone marrow mesenchymal stem cells, platelet-rich plasma and nanohydroxyapatite-type I collagen beads were integral parts of biomimetic bone substitutes for bone regeneration. J Tissue Eng Regen Med. 7:841–854. 2013. View Article : Google Scholar : PubMed/NCBI
7	Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE and Georgeff KR: Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 85:638–646. 1998. View Article : Google Scholar : PubMed/NCBI
8	Kim ES, Kim JJ and Park EJ: Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft material. J Adv Prosthodont. 2:7–13. 2010. View Article : Google Scholar : PubMed/NCBI
9	Yokota K, Ishida O, Sunagawa T, et al: Platelet-rich plasma accelerated surgical angio-genesis in vascular-implanted necrotic bone: an experimental study in rabbits. Acta Orthop. 79:106–110. 2008. View Article : Google Scholar : PubMed/NCBI
10	Tao H, Zhang C, Zeng B, Yuan T, Xu J and Song W: Experimental study on the treatment of femur head necrosis with tricalcium phosphate and platelet-rich plasma. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 19:170–173. 2005.(In Chinese).
11	Latalski M, Elbatrawy YA, Thabet AM, et al: Enhancing bone healing during distraction osteogenesis with platelet-rich plasma. Injury. 42:821–824. 2011. View Article : Google Scholar : PubMed/NCBI
12	Curi MM, Cossolin GS, Koga DH, et al: Bisphosphonate-related osteonecrosis of the jaws - an initial case series report of treatment combining partial bone resection and autologous platelet-rich plasma. J Oral Maxillofac Surg. 69:2465–2472. 2011. View Article : Google Scholar : PubMed/NCBI
13	Scala M, Gipponi M, Mereu P, et al: Regeneration of mandibular osteoradionecrosis defect with platelet rich plasma gel. In Vivo. 24:889–893. 2010.PubMed/NCBI
14	Klein-Nulend J, van der Plas A, Semeins CM, et al: Sensitivity of osteocytes to biomechanical stress in vitro. FASEB J. 9:441–445. 1995.PubMed/NCBI
15	Owan I, Burr DB, Turner CH, et al: Mechanotransduction in bone: osteoblasts are more responsive to fluid forces than mechanical strain. Am J Physiol. 273:C810–C815. 1997.PubMed/NCBI
16	Bakker AD, Soejima K, Klein-Nulend J and Burger EH: The production of nitric oxide and prostaglandin E(2) by primary bone cells is shear stress dependent. J Biomech. 34:671–677. 2001. View Article : Google Scholar : PubMed/NCBI
17	Wang Y, Kim UJ, Blasioli DJ, et al: In vitro cartilage tissue engineering with 3D porous aqueous-derived silk scaffolds and mesenchymal stem cells. Biomaterials. 26:7082–7094. 2005. View Article : Google Scholar : PubMed/NCBI
18	Wang W, Itaka K, Ohba S, et al: 3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells. Biomaterials. 30:2705–2715. 2009. View Article : Google Scholar : PubMed/NCBI
19	Sun S, Ren Q, Wang D, Zhang L, Wu S and Sun XT: Repairing cartilage defects using chondrocyte and osteoblast composites developed using a bioreactor. Chin Med J (Engl). 124:758–763. 2011.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Construction of tissue‑engineered bone using a bioreactor and platelet‑rich plasma

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