Expression of vascular endothelial growth factor and basic fibroblast growth factor in acute rejection reaction 
following rat orthotopic liver transplantation

Zhang,Changsong; Yang,Guangshun; Lu,Dewen; Ling,Yang; Chen,Guihua; Zhou,Tianbao

doi:10.3892/etm.2014.1779

August-2014 Volume 8 Issue 2

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August-2014 Volume 8 Issue 2

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Article

Expression of vascular endothelial growth factor and basic fibroblast growth factor in acute rejection reaction following rat orthotopic liver transplantation

Authors:
- Changsong Zhang
- Guangshun Yang
- Dewen Lu
- Yang Ling
- Guihua Chen
- Tianbao Zhou
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Affiliations: Clinical Oncology Laboratory, Changzhou Tumor Hospital, Medical College of Soochow University, Changzhou, Jiangsu 213002, P.R. China, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, P.R. China, The Affiliated Yinzhou Hospital, Ningbo University School of Medicine, Ningbo, Zhejiang 315040, P.R. China, Hepatic Surgery Center, Third Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China, Hepatobiliary Surgery Centre, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, P.R. China
Pages: 483-487
|
Published online on: June 11, 2014

https://doi.org/10.3892/etm.2014.1779
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Abstract

The aim of the present study was to investigate the expression levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in acute rejection reaction (ARR) following orthotopic liver transplantation in a rat model. Serum VEGF and bFGF levels were detected using ELISA, and their expression levels in liver and spleen tissues were determined using immunohistochemistry. The mRNA expression levels of VEGF and bFGF were detected by conducting a quantitative polymerase chain reaction during the ARR following orthotopic liver transplantation. The expression levels of VEGF and bFGF in the serum 3 days following liver transplantation were significantly higher compared with those in the other groups (1 and 7 days following transplantation; P<0.01). In addition, the numbers of cells in the liver tissue that were shown to be positive for the expression VEGF and bFGF using immunohistochemistry were significantly higher 3 days following transplantation than at the other time points (P<0.0001). Furthermore, the numbers of cells positive for VEGF and bFGF expression in the spleen detected 3 days following the transplantation surgery were also significantly higher compared with those at the other time points (P<0.01). VEGF and bFGF mRNA expression levels were also increased from 1 day following the surgery and reached a peak at day 3, prior to declining gradually and remaining at a relatively high level. VEGF and bFGF mRNA expression levels changed dynamically, by peaking and then declining, in ARR following orthotopic liver transplantation. These changes may have an important impact on angiogenesis and the inflammatory reaction, and the identification of these changes increases the current understanding of ARR following orthotopic liver transplantation.

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1	Renna-Molajoni E, Cinti P, Elia L, et al: Mechanism of liver allograft rejection: indirect allorecognition. Transplant Proc. 31:409–410. 1999. View Article : Google Scholar : PubMed/NCBI
2	Zhou TB and Yang GS: Roles of vascular endothelial growth factor in acute rejection reaction following liver transplantation. Transpl Immunol. 25:207–209. 2011. View Article : Google Scholar : PubMed/NCBI
3	Oh JY, Kim MK, Shin MS, et al: The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Stem Cells. 26:1047–1055. 2008. View Article : Google Scholar : PubMed/NCBI
4	Reinders ME, Fang JC, Wong W, Ganz P and Briscoe DM: Expression patterns of vascular endothelial growth factor in human cardiac allografts: association with rejection. Transplantation. 76:224–230. 2003. View Article : Google Scholar : PubMed/NCBI
5	Kraft A, Weindel K, Ochs A, et al: Vascular endothelial growth factor in the sera and effusions of patients with malignant and nonmalignant disease. Cancer. 85:178–187. 1999. View Article : Google Scholar : PubMed/NCBI
6	Pilmore HL, Eris JM, Painter DM, Bishop GA and McCaughan GW: Vascular endothelial growth factor expression in human chronic renal allograft rejection. Transplantation. 67:929–933. 1999. View Article : Google Scholar : PubMed/NCBI
7	Kuninaka T, Senga Y, Senga H and Weiner M: Nature of enhanced mitochondrial oxidative metabolism by a calf blood extract. J Cell Physiol. 146:148–155. 1991. View Article : Google Scholar : PubMed/NCBI
8	Kamada N and Calne RY: A surgical experience with five hundred thirty liver transplants in the rat. Surgery. 93:64–69. 1983.PubMed/NCBI
9	Solez K and Racusen LC: The Banff classification revisited. Kidney Int. 83:201–206. 2013. View Article : Google Scholar : PubMed/NCBI
10	No authors listed. An international panel. Banff schema for grading liver allograft rejection: an international consensus document. Hepatology. 25:658–663. 1997. View Article : Google Scholar : PubMed/NCBI
11	Bayliss J, Maguire JA, Bailey M, et al: Increased vascular endothelial growth factor mRNA in endomyocardial biopsies from allografts demonstrating severe acute rejection: a longitudinal study. Transpl Immunol. 18:264–274. 2008. View Article : Google Scholar
12	Chow NH, Hsu PI, Lin XZ, et al: Expression of vascular endothelial growth factor in normal liver and hepatocellular carcinoma: an immunohistochemical study. Hum Pathol. 28:698–703. 1997. View Article : Google Scholar : PubMed/NCBI
13	Tambur AR, Pamboukian S, Costanzo MR and Heroux A: Genetic polymorphism in platelet-derived growth factor and vascular endothelial growth factor are significantly associated with cardiac allograft vasculopathy. J Heart Lung Transplant. 25:690–698. 2006. View Article : Google Scholar
14	Conti A, Scala S, D’Agostino P, et al: Wide gene expression profiling of ischemia-reperfusion injury in human liver transplantation. Liver Transpl. 13:99–113. 2007. View Article : Google Scholar : PubMed/NCBI
15	Borozan I, Chen L, Sun J, et al: Gene expression profiling of acute liver stress during living donor liver transplantation. Am J Transplant. 6:806–824. 2006. View Article : Google Scholar : PubMed/NCBI
16	Fallsehr C, Zapletal C, Kremer M, Demir R, von Knebel Doeberitz M and Klar E: Identification of differentially expressed genes after partial rat liver ischemia/reperfusion by suppression subtractive hybridization. World J Gastroenterol. 11:1303–1316. 2005. View Article : Google Scholar
17	Winn R, Vedder N, Ramamoorthy C, Sharar S and Harlan J: Endothelial and leukocyte adhesion molecules in inflammation and disease. Blood Coagul Fibrinolysis. 9(Suppl 2): S17–S23. 1998.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression of vascular endothelial growth factor and basic fibroblast growth factor in acute rejection reaction following rat orthotopic liver transplantation

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