Hydrogen sulfide reduces kidney injury due to urinary‑derived sepsis by inhibiting NF-κB expression, decreasing TNF-α levels and increasing IL-10 levels

Chen,Xian; Xu,Wujun; Wang,Yi; Luo,Hongmei; Quan,Suqin; Zhou,Jing; Yang,Ning; Zhang,Tao; Wu,Lei; Liu,Jun; Long,Xiangyang; Zhu,Neng; Xie,Huang; Luo,Zhigang

doi:10.3892/etm.2014.1781

August-2014 Volume 8 Issue 2

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August-2014 Volume 8 Issue 2

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Article

Hydrogen sulfide reduces kidney injury due to urinary‑derived sepsis by inhibiting NF-κB expression, decreasing TNF-α levels and increasing IL-10 levels

Authors:
- Xian Chen
- Wujun Xu
- Yi Wang
- Hongmei Luo
- Suqin Quan
- Jing Zhou
- Ning Yang
- Tao Zhang
- Lei Wu
- Jun Liu
- Xiangyang Long
- Neng Zhu
- Huang Xie
- Zhigang Luo
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Affiliations: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China, Department of Histology and Embryology, University of South China, Hengyang, Hunan 421001, P.R. China
Pages: 464-470
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Published online on: June 12, 2014

https://doi.org/10.3892/etm.2014.1781
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Abstract

The present study aimed to investigate the effect of hydrogen sulfide (H2S) on kidney injury induced by urinary‑derived sepsis. Rabbits were randomly divided into control, sham, sepsis, NaHS 2.8 µmol/kg and NaHS 8.4 µmol/kg groups, with six rabbits in each group. Upper urinary tract obstruction and acute infection was induced to establish the sepsis model. Blood was collected to carry out a white blood cell (WBC) count, and creatinine (Cr) and blood urea nitrogen (BUN) analysis. Morphological changes were observed by hematoxylin and eosin (H&E) staining and transmission electron microscopy. Immunohistochemical staining was used to detect the expression levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑10 and nuclear factor κ-light-chain-enhancer of activated B cells (NF‑κB). Cystathionine‑γ‑lyase (CSE) activity was measured by the spectrophotometric methylene blue method and the blood H2S concentration was measured by deproteinization. WBC, Cr and BUN levels were significantly elevated in the sepsis group compared with those in the control group (P<0.05). Following treatment with NaHS, the WBC, Cr and BUN levels were significantly decreased in the NaHS groups compared with those in the sepsis group (P<0.05). The pathological features of kidney injury were also alleviated by NaHS. In the sepsis group, the levels of TNF‑α, IL‑10 and NF‑κB were significantly increased compared with those in the control group (P<0.05). In the NaHS groups, the TNF‑α and NF‑κB levels were significantly reduced whereas the IL‑10 level was significantly increased compared with the respective levels in the sepsis group (P<0.05). The H2S concentration was significantly decreased in the sepsis group and this reduction was attenuated in the NaHS groups (P<0.05). Furthermore, the NaHS 8.4 µmol/kg dose revealed a more potent effect than the NaHS 2.8 µmol/kg dose. Thus, exogenous H2S reduced kidney injury from urinary‑derived sepsis by decreasing the levels of NF‑κB and TNF‑α, and increasing the level of IL‑10.

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1	Farshad S, Ranjbar R, Japoni A, et al: Microbial susceptibility, virulence factors, and plasmid profiles of uropathogenic Escherichia coli strains isolated from children in Jahrom, Iran. Arch Iran Med. 15:312–316. 2012.PubMed/NCBI
2	Bagshaw SM, George C and Bellomo R: ANZICS Database Management Committee: Early acute kidney injury and sepsis: a multicentre evaluation. Crit Care. 12:R472008. View Article : Google Scholar : PubMed/NCBI
3	Sadik NA, Mohamed WA and Ahmed MI: The association of receptor of advanced glycated end products and inflammatory mediators contributes to endothelial dysfunction in a prospective study of acute kidney injury patients with sepsis. Mol Cell Biochem. 359:73–81. 2012. View Article : Google Scholar
4	Souza AC, Volpini RA, Shimizu MH, et al: Erythropoietin prevents sepsis-related acute kidney injury in rats by inhibiting NF-κB and upregulating endothelial nitric oxide synthase. Am J Physiol Renal Physiol. 302:F1045–1054. 2012.PubMed/NCBI
5	Makkonen J, Pietiläinen KH, Rissanen A, et al: Genetic factors contribute to variation in serum alanine aminotransferase activity independent of obesity and alcohol: a study in monozygotic and dizygotic twins. J Hepatol. 50:1035–1042. 2009. View Article : Google Scholar
6	Kimura H: Hydrogen sulfide: its production, release and functions. Amino Acids. 41:113–121. 2011. View Article : Google Scholar : PubMed/NCBI
7	Calvert JW, Coetzee WA and Lefer DJ: Novel insights into hydrogen sulfide-mediated cytoprotection. Antioxid Redox Signal. 12:1203–1217. 2010. View Article : Google Scholar : PubMed/NCBI
8	Zhou CF and Tang XQ: Hydrogen sulfide and nervous system regulation. Chin Med J (Engl). 124:3576–3582. 2011.PubMed/NCBI
9	Bełtowski J: Hypoxia in the renal medulla: implications for hydrogen sulfide signaling. J Pharmacol Exp Ther. 334:358–363. 2010.PubMed/NCBI
10	Pan LL, Liu XH, Gong QH, et al: Hydrogen sulfide attenuated tumor necrosis factor-α-induced inflammatory signaling and dysfunction in vascular endothelial cells. PLoS One. 6:e197662011.
11	Tokuda K, Kida K, Marutani E, et al: Inhaled hydrogen sulfide prevents endotoxin-induced systemic inflammation and improves survival by altering sulfide metabolism in mice. Antioxid Redox Signal. 17:11–21. 2012. View Article : Google Scholar : PubMed/NCBI
12	Ang SF, Sio SW, Moochhala SM, et al: Hydrogen sulfide upregulates cyclooxygenase-2 and prostaglandin E metabolite in sepsis-evoked acute lung injury via transient receptor potential vanilloid type 1 channel activation. J Immunol. 187:4778–4787. 2011. View Article : Google Scholar
13	Chattopadhyay M, Kodela R, Nath N, et al: Hydrogen sulfide-releasing aspirin suppresses NF-κB signaling in estrogen receptor negative breast cancer cells in vitro and in vivo. Biochem Pharmacol. 83:723–732. 2012.PubMed/NCBI
14	Sen N, Paul BD, Gadalla MM, et al: Hydrogen sulfide-linked sulfhydration of NF-κB mediates its antiapoptotic actions. Mol Cell. 45:13–24. 2012.PubMed/NCBI
15	Chen X, Wang Y, Luo H, et al: Ulinastatin reduces urinary sepsis-related inflammation by upregulating IL-10 and downregulating TNF α levels. Mol Med Rep. 8:29–34. 2013.PubMed/NCBI
16	Payen D, Lukaszewicz AC, Legrand M, et al: A multicenter study of acute kidney injury in severe sepsis and septic shock: association with inflammatory phenotype and HLA genotype. PLoS One. 7:e358382012. View Article : Google Scholar : PubMed/NCBI
17	Li L, Salto-Tellez M, Tan CH, et al: GYY4137, a novel hydrogen sulfide-releasing molecule, protects against endotoxic shock in the rat. Free Radic Biol Med. 47:103–113. 2009. View Article : Google Scholar
18	Yang C, Yang Z, Zhang M, et al: Hydrogen sulfide protects against chemical hypoxia-induced cytotoxicity and inflammation in HaCaT cells through inhibition of ROS/NF-κB/COX-2 pathway. PLoS One. 6:e219712011.PubMed/NCBI
19	Pan LL, Liu XH, Gong QH, et al: Hydrogen sulfide attenuated tumor necrosis factor-α-induced inflammatory signaling and dysfunction in vascular endothelial cells. PLoS One. 6:e197662011.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Hydrogen sulfide reduces kidney injury due to urinary‑derived sepsis by inhibiting NF-κB expression, decreasing TNF-α levels and increasing IL-10 levels

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