Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2014 Volume 8 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2014 Volume 8 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Identification and characterization of dermatophyte species and strains with PCR amplification

  • Authors:
    • Guofang Liu
    • Chenghua He
    • Haibin Zhang
  • View Affiliations / Copyright

    Affiliations: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China, Department of Animal Husbandry and Veterinary Medicine, Jiangsu Polytechnic College of Agriculture and Forestry, Zhenjiang, Jiangsu 212400, P.R. China
  • Pages: 545-550
    |
    Published online on: June 13, 2014
       https://doi.org/10.3892/etm.2014.1785
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to use two polymerase chain reaction (PCR) methods, with (GACA)4 and non‑transcribed spacer (NTS) as primers, to identify and characterize dermatophyte isolates from dogs and cats to a species and strain level. A total of 45 isolates from nine dermatophyte species were collected from pet dogs and cats and subjected to PCR amplification with the microsatellite primer (GACA)4. Dermatophyte strains of three of the same species collected from four cities were subjected to PCR amplification with the NTS primer set. These two PCR methods were applied to identify and characterize the dermatophyte isolates to a species and strain level. Regional differences among the strain specificities were also examined. The results from PCR with (GACA)4 demonstrated that strains from the same species produced similar PCR product band patterns. In addition, these patterns differed among species, indicating that (GACA)4 primer‑based PCR was able to distinguish between the various dermatophyte species. By contrast, dermatophyte isolates and/or strains within the same species revealed various band patterns with NTS‑based PCR. In addition, the results indicated that regional differences contributed to the variations in PCR product band patterns. Therefore, the results of the present study indicate that the NTS‑based PCR method is efficient in distinguishing dermatophytes to the strain level, while a combination of (GACA)4 and NTS primer‑based PCR methods is able to clarify dermatophyte isolates to a species and strain level. The present study provides information concerning the identification of pathogenic fungi and the epidemiological characteristics of fungal skin diseases.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Moretti A, Agnetti F, Mancianti F, et al: Dermatophytosis in animals: epidemiological, clinical and zoonotic aspects. G Ital Dermatol Venereol. 148:563–572. 2013.PubMed/NCBI

2 

Gaedigk A, Gaedigk R and Abdel-Rahman SM: Genetic heterogeneity in the rRNA gene locus of Trichophyton tonsurans. J Clin Microbiol. 41:5478–5487. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Kac G: Molecular approaches to the study of dermatophytes. Med Mycol. 38:329–336. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Shehata AS, Mukherjee PK, Aboulatta HN, et al: Single-step PCR using (GACA)4 primer: utility for rapid identification of dermatophyte species and strains. J Clin Microbiol. 46:2641–2645. 2008.PubMed/NCBI

5 

Zhu H, Wen H and Liao W: Identification of Trichophyton rubrum by PCR fingerprinting. Chin Med J (Engl). 115:1218–1220. 2002.

6 

Orsini M and Romano-Spica V: A microwave-based method for nucleic acid isolation from environmental samples. Lett Appl Microbiol. 33:17–20. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Cassago A, Panepucci R, Baião A and Henrique-Silva F: Cellophane based mini-prep method for DNA extraction from the filamentous fungus Trichoderma reesei. BMC Microbiol. 2:142002. View Article : Google Scholar : PubMed/NCBI

8 

Kac G, Bougnoux ME, Feuilhade De Chauvin M, Sene S and Derouin F: Genetic diversity among Trichophyton mentagrophytes isolates using random amplified polymorphic DNA method. Br J Dermatol. 140:839–844. 1999.

9 

Summerbell RC, Haugland RA, Li A and Gupta AK: rRNA gene internal transcribed spacer 1 and 2 sequences of asexual, anthropophilic dermatophytes related to Trichophyton rubrum. J Clin Microbiol. 37:4005–4011. 1999.PubMed/NCBI

10 

Makimura K, Mochizuki T, Hasegawa A, et al: Phylogenetic classification of Trichophyton mentagrophytes complex strains based on DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions. J Clin Microbiol. 36:2629–2633. 1998.

11 

Apodaca G and McKerrow JH: Regulation of Trichophyton rubrum proteolytic activity. Infect Immun. 57:3081–3090. 1989.PubMed/NCBI

12 

Jackson CJ, Barton RC, Kelly SL and Evans EG: Strain identification of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer. J Clin Microbiol. 38:4527–4534. 2000.

13 

Mochizuki T, Kawasaki M, Ishizaki H, et al: Molecular epidemiology of Arthroderma benhamiae, an emerging pathogen of dermatophytoses in Japan, by polymorphisms of the non-transcribed spacer region of the ribosomal DNA. J Dermatol Sci. 27:14–20. 2001.

14 

Zhu HM, Liao WQ and Dai JX: PCR fingerprint analysis of Trichophyton rubrum and Trichophyton mentagrophytes. Chin J Dermatol. 33:3492000.(In Chinese).

15 

Shin JH, Sung JH, Park SJ, et al: Species identification and strain differentiation of dermatophyte fungi using polymerase chain reaction amplification and restriction enzyme analysis. J Am Acad Dermatol. 48:857–865. 2003. View Article : Google Scholar

16 

Li Q, Liu WD, Yang GL and Li CJ: DNA typing of Trichophyton rubrum. Chin J Dermatol. 35:352–354. 2002.(In Chinese).

17 

Jackson CJ, Mochizuki T and Barton RC: PCR fingerprinting of Trichophyton mentagrophytes var. interdigitale using polymorphic subrepeat loci in the rDNA nontranscribed spacer. J Med Microbiol. 55:1349–1355. 2006.

18 

Takeda K, Nishibu A, Anzawa K and Mochizuki T: Molecular epidemiology of a major subgroup of Arthroderma benhamiae isolated in Japan by restriction fragment length polymorphism analysis of the non-transcribed spacer region of ribosomal RNA gene. Jpn J Infect Dis. 65:233–239. 2012.PubMed/NCBI

19 

Fan JF, Li HJ, Suo JJ, Li RY and Wan Z: Strain typing in Trichophyton rubrum. Academic Journal of Chinese Pla Medical School. 25:225–226. 2004.(In Chinese).

20 

Nishio K, Kawasaki M and Ishizaki H: Phylogeny of the genera Trichophyton using mitochondrial DNA analysis. Mycopathologia. 117:127–132. 1992.

21 

Jackson CJ, Barton RC and Evans EG: Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions. J Clin Microbiol. 37:931–936. 1999.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu G, He C and Zhang H: Identification and characterization of dermatophyte species and strains with PCR amplification. Exp Ther Med 8: 545-550, 2014.
APA
Liu, G., He, C., & Zhang, H. (2014). Identification and characterization of dermatophyte species and strains with PCR amplification. Experimental and Therapeutic Medicine, 8, 545-550. https://doi.org/10.3892/etm.2014.1785
MLA
Liu, G., He, C., Zhang, H."Identification and characterization of dermatophyte species and strains with PCR amplification". Experimental and Therapeutic Medicine 8.2 (2014): 545-550.
Chicago
Liu, G., He, C., Zhang, H."Identification and characterization of dermatophyte species and strains with PCR amplification". Experimental and Therapeutic Medicine 8, no. 2 (2014): 545-550. https://doi.org/10.3892/etm.2014.1785
Copy and paste a formatted citation
x
Spandidos Publications style
Liu G, He C and Zhang H: Identification and characterization of dermatophyte species and strains with PCR amplification. Exp Ther Med 8: 545-550, 2014.
APA
Liu, G., He, C., & Zhang, H. (2014). Identification and characterization of dermatophyte species and strains with PCR amplification. Experimental and Therapeutic Medicine, 8, 545-550. https://doi.org/10.3892/etm.2014.1785
MLA
Liu, G., He, C., Zhang, H."Identification and characterization of dermatophyte species and strains with PCR amplification". Experimental and Therapeutic Medicine 8.2 (2014): 545-550.
Chicago
Liu, G., He, C., Zhang, H."Identification and characterization of dermatophyte species and strains with PCR amplification". Experimental and Therapeutic Medicine 8, no. 2 (2014): 545-550. https://doi.org/10.3892/etm.2014.1785
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team