microRNA‑181 promotes prostate cancer cell proliferation by regulating DAX‑1 expression

Tong,Shi‑Jun; Liu,Jun; Wang,Xiang; Qu,Lian‑Xi

doi:10.3892/etm.2014.1846

October 2014 Volume 8 Issue 4

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October 2014 Volume 8 Issue 4

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Article

microRNA‑181 promotes prostate cancer cell proliferation by regulating DAX‑1 expression

Authors:
- Shi‑Jun Tong
- Jun Liu
- Xiang Wang
- Lian‑Xi Qu
View Affiliations / Copyright

Affiliations: Department of Urologic Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China
Pages: 1296-1300
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Published online on: July 16, 2014

https://doi.org/10.3892/etm.2014.1846
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Abstract

microRNAs (miRNAs) are a class of short noncoding RNA molecules that have a critical role in the initiation and progression of types of human cancer, including prostate cancer. In the present study, the expression of miR‑181 in prostate cancer tissues was evaluated and was demonstrated to be significantly upregulated in prostate cancer tissues compared with that in adjacent normal tissues. The results of in vitro MTT and BrdU incorporation assays, as well as cell‑cycle analysis, indicated that miR‑181 overexpression markedly promoted the proliferation of LNCaP cells. Furthermore, miR‑181 overexpression was found to promote the progression of LNCaP tumor growth in nude mice. Mechanistic studies demonstrated that dosage‑sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (DAX‑1), a negative regulator of androgen receptor in prostate cancer, was inhibited by miR‑181 overexpression. Therefore, the results from the present study suggest that miR‑181 functions as a growth‑suppressive miRNA during prostate cancer development.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

microRNA‑181 promotes prostate cancer cell proliferation by regulating DAX‑1 expression

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