Interferon‑γ and its pathway‑associated gene expression in the vaginal tissue of premenopausal females with pelvic organ prolapse

Zhao,Bing; Yan,Jianguo; Wu,Huiyan; Zhou,Yali; Xu,Dongmei; Hu,Mengcai; Cui,Shihong

doi:10.3892/etm.2014.1868

October 2014 Volume 8 Issue 4

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October 2014 Volume 8 Issue 4

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Article

Interferon‑γ and its pathway‑associated gene expression in the vaginal tissue of premenopausal females with pelvic organ prolapse

Authors:
- Bing Zhao
- Jianguo Yan
- Huiyan Wu
- Yali Zhou
- Dongmei Xu
- Mengcai Hu
- Shihong Cui
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Affiliations: The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Anatomy, Basic Medical College, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Pages: 1145-1149
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Published online on: July 29, 2014

https://doi.org/10.3892/etm.2014.1868
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Abstract

Interferon (IFN)‑γ is a potent proinflammatory molecule. However, few studies have investigated the expression levels of IFN‑γ during pelvic organ prolapse (POP). In the present study, the expression levels and tissue localization of IFN‑γ and its pathway‑associated genes were detected in the vaginal walls of premenopausal females with POP and asymptomatic controls using quantitative polymerase chain reaction and immunohistochemistry. When compared with the matched controls, an 8.6‑fold increase in IFN‑γ, 3.8‑fold increase in IFN‑γ receptor (IFNGR)1, 2.6‑fold increase in IFNGR2, 3.4‑fold increase in signal transducer and activator of transcription‑1, 2.2‑fold increase in janus kinase‑1 and 5.1‑fold increase in nuclear factor (NF)‑κB mRNA expression levels were observed in the females with premenopausal POP. In all the females with POP, higher mRNA expression levels of IFN‑γ and its receptors were observed when compared with the controls. Expression levels of all the proteins were detected by immunohistochemistry, and the results demonstrated higher staining for IFN‑γ, IFNGRs and pathway‑associated genes in females with POP. Therefore, the results indicated that IFN‑γ may be used as an inflammatory marker for POP development, and is associated with NF‑κB.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Interferon‑γ and its pathway‑associated gene expression in the vaginal tissue of premenopausal females with pelvic organ prolapse

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