Interferon‑γ and its pathway‑associated gene expression in the vaginal tissue of premenopausal females with pelvic organ prolapse

  • Authors:
    • Bing Zhao
    • Jianguo Yan
    • Huiyan Wu
    • Yali Zhou
    • Dongmei  Xu
    • Mengcai Hu
    • Shihong Cui
  • View Affiliations

  • Published online on: July 29, 2014     https://doi.org/10.3892/etm.2014.1868
  • Pages: 1145-1149
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Abstract

Interferon (IFN)‑γ is a potent proinflammatory molecule. However, few studies have investigated the expression levels of IFN‑γ during pelvic organ prolapse (POP). In the present study, the expression levels and tissue localization of IFN‑γ and its pathway‑associated genes were detected in the vaginal walls of premenopausal females with POP and asymptomatic controls using quantitative polymerase chain reaction and immunohistochemistry. When compared with the matched controls, an 8.6‑fold increase in IFN‑γ, 3.8‑fold increase in IFN‑γ receptor (IFNGR)1, 2.6‑fold increase in IFNGR2, 3.4‑fold increase in signal transducer and activator of transcription‑1, 2.2‑fold increase in janus kinase‑1 and 5.1‑fold increase in nuclear factor (NF)‑κB mRNA expression levels were observed in the females with premenopausal POP. In all the females with POP, higher mRNA expression levels of IFN‑γ and its receptors were observed when compared with the controls. Expression levels of all the proteins were detected by immunohistochemistry, and the results demonstrated higher staining for IFN‑γ, IFNGRs and pathway‑associated genes in females with POP. Therefore, the results indicated that IFN‑γ may be used as an inflammatory marker for POP development, and is associated with NF‑κB.
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October 2014
Volume 8 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhao B, Yan J, Wu H, Zhou Y, Xu D, Hu M and Cui S: Interferon‑γ and its pathway‑associated gene expression in the vaginal tissue of premenopausal females with pelvic organ prolapse. Exp Ther Med 8: 1145-1149, 2014
APA
Zhao, B., Yan, J., Wu, H., Zhou, Y., Xu, D., Hu, M., & Cui, S. (2014). Interferon‑γ and its pathway‑associated gene expression in the vaginal tissue of premenopausal females with pelvic organ prolapse. Experimental and Therapeutic Medicine, 8, 1145-1149. https://doi.org/10.3892/etm.2014.1868
MLA
Zhao, B., Yan, J., Wu, H., Zhou, Y., Xu, D., Hu, M., Cui, S."Interferon‑γ and its pathway‑associated gene expression in the vaginal tissue of premenopausal females with pelvic organ prolapse". Experimental and Therapeutic Medicine 8.4 (2014): 1145-1149.
Chicago
Zhao, B., Yan, J., Wu, H., Zhou, Y., Xu, D., Hu, M., Cui, S."Interferon‑γ and its pathway‑associated gene expression in the vaginal tissue of premenopausal females with pelvic organ prolapse". Experimental and Therapeutic Medicine 8, no. 4 (2014): 1145-1149. https://doi.org/10.3892/etm.2014.1868