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Article

Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c

  • Authors:
    • Liang Ma
    • Wusheng Li
  • View Affiliations / Copyright

    Affiliations: Department of Anorectal Surgery, The Affiliated Traditional Chinese Medicine Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P.R. China
  • Pages: 1225-1228
    |
    Published online on: August 11, 2014
       https://doi.org/10.3892/etm.2014.1900
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Abstract

In this study, the effect of emodin and its mechanism of action were investigated in LOVO colorectal cancer cells. Cell growth was determined using a Cell Counting kit‑8 assay, and the results demonstrated that emodin significantly inhibited the growth of LOVO cells in a concentration‑dependent manner. In order to investigate the anticancer mechanism of emodin, reverse transcription polymerase chain reaction assays were performed to determine the B‑cell lymphoma‑2 (Bcl‑2)/Bcl‑2‑associated X protein (Bax) expression ratio in LOVO colorectal cancer cells following treatment with emodin. The results showed that emodin induced a significant increase in the Bax expression level and a marked reduction of the Bcl‑2 expression level in LOVO cells. In addition, emodin was found to have an inhibitory effect on the mitochondrial membrane potential and the results from the western blot analysis revealed that cytochrome c was released from the mitochondria to the cytoplasm. In combination, these results suggest that emodin inhibits cancer cell growth via the regulation of the Bcl‑2/Bax ratio and by its effect on the mitochondrial apoptosis pathway.
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1 

Ma YS, Weng SW, Lin MW, et al: Antitumor effects of emodin on LS1034 human colon cancer cells in vitro and in vivo: roles of apoptotic cell death and LS1034 tumor xenografts model. Food Chem Toxicol. 50:1271–1278. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Jing X, Ueki N, Cheng J, Imanishi H and Hada T: Induction of apoptosis in hepatocellular carcinoma cell lines by emodin. Jpn J Cancer Res. 93:874–882. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Liu A, Chen H, Wei W, et al: Antiproliferative and antimetastatic effects of emodin on human pancreatic cancer. Oncol Rep. 26:81–89. 2011.PubMed/NCBI

4 

Li J, Liu P, Mao H, Wanga A and Zhang X: Emodin sensitizes paclitaxel-resistant human ovarian cancer cells to paclitaxel-induced apoptosis in vitro. Oncol Rep. 21:1605–1610. 2009.PubMed/NCBI

5 

Ko JC, Su YJ, Lin ST, et al: Suppression of ERCC1 and Rad51 expression through ERK1/2 inactivation is essential in emodin-mediated cytotoxicity in human non-small cell lung cancer cells. Biochem Pharmacol. 79:655–664. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Wang CG, Yang JQ, Liu BZ, et al: Anti-tumor activity of emodin against human chronic myelocytic leukemia K562 cell lines in vitro and in vivo. Eur J Pharmacol. 627:33–41. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Shang T, Joseph J, Hillard CJ and Kalyanaraman B: Death-associated protein kinase as a sensor of mitochondrial membrane potential: role of lysosome in mitochondrial toxin-induced cell death. J Biol Chem. 280:34644–34653. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Bae JH, Park JW and Kwon TK: Ruthenium red, inhibitor of mitochondrial Ca2+ uniporter, inhibits curcumin-induced apoptosis via the prevention of intracellular Ca2+ depletion and cytochrome c release. Biochem Biophys Res Commun. 303:1073–1079. 2003.PubMed/NCBI

9 

Grishko V, Rachek L, Musiyenko S, Ledoux SP and Wilson GL: Involvement of mtDNA damage in free fatty acid-induced apoptosis. Free Radic Biol Med. 38:755–762. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Xu JD, Liu S, Wang W, et al: Emodin induces chloride secretion in rat distal colon through activation of mast cells and enteric neurons. Br J Pharmacol. 165:197–207. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Chen H, Wei W, Guo Y, et al: Enhanced effect of gemcitabine by emodin against pancreatic cancer in vivo via cytochrome C-regulated apoptosis. Oncol Rep. 25:1253–1261. 2011.PubMed/NCBI

12 

Reed JC: Bcl-2 family proteins. Oncogene. 17:3225–3236. 1998. View Article : Google Scholar

13 

Wang JB, Qi LL, Zheng SD and Wu TX: Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells. J Zhejiang Univ Sci B. 10:93–102. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Pettersson F, Dalgleish AG, Bissonnette RP and Colston KW: Retinoids cause apoptosis in pancreatic cancer cells via activation of RAR-gamma and altered expression of Bcl-2/Bax. Br J Cancer. 87:555–561. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Wang X: The expanding role of mitochondria in apoptosis. Genes Dev. 15:2922–2933. 2001.PubMed/NCBI

16 

Wang YX, Yu H, Zhang YY, et al: Emodin induces apoptosis of human cervical cancer HeLa cells via intrinsic mitochondrial and extrinsic death receptor pathway. Cancer Cell Int. 13:712013. View Article : Google Scholar : PubMed/NCBI

17 

Guo LD, Chen XJ, Hu YH, Yu ZJ, Wang D and Liu JZ: Curcumin inhibits proliferation and induces apoptosis of human colorectal cancer cells by activating the mitochondria apoptotic pathway. Phytother Res. 27:422–430. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Jin P, Wu ZT, Li SR, Li SJ, Wang JH, Wang ZH, Lu JG, Cui XJ, Han Y, Rao J and Sheng JQ: Colorectal cancer screening with fecal occult blood test: A 22-year cohort study. Oncol Lett. 6:576–582. 2013.PubMed/NCBI

19 

Andre T, Boni C and Mounedji-Boudiaf L: Oxaliplatin, fluorouracil, and leucovorin as adjuvant treatment for colon cancer. N Engl J Med. 350:2343–2351. 2004. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Ma L and Li W: Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c. Exp Ther Med 8: 1225-1228, 2014.
APA
Ma, L., & Li, W. (2014). Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c. Experimental and Therapeutic Medicine, 8, 1225-1228. https://doi.org/10.3892/etm.2014.1900
MLA
Ma, L., Li, W."Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c". Experimental and Therapeutic Medicine 8.4 (2014): 1225-1228.
Chicago
Ma, L., Li, W."Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c". Experimental and Therapeutic Medicine 8, no. 4 (2014): 1225-1228. https://doi.org/10.3892/etm.2014.1900
Copy and paste a formatted citation
x
Spandidos Publications style
Ma L and Li W: Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c. Exp Ther Med 8: 1225-1228, 2014.
APA
Ma, L., & Li, W. (2014). Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c. Experimental and Therapeutic Medicine, 8, 1225-1228. https://doi.org/10.3892/etm.2014.1900
MLA
Ma, L., Li, W."Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c". Experimental and Therapeutic Medicine 8.4 (2014): 1225-1228.
Chicago
Ma, L., Li, W."Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c". Experimental and Therapeutic Medicine 8, no. 4 (2014): 1225-1228. https://doi.org/10.3892/etm.2014.1900
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