Expression of a novel splice variant of FRMD7 in developing human fetal brains that is upregulated upon the differentiation of NT2 cells

  • Authors:
    • Yingzhi Li
    • Jiali Pu
    • Baorong Zhang
  • View Affiliations

  • Published online on: August 19, 2014     https://doi.org/10.3892/etm.2014.1916
  • Pages: 1131-1136
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Abstract

FRMD7 mutations are associated with X‑linked idiopathic congenital nystagmus (ICN); however, the underlying mechanisms whereby mutations of FRMD7 lead to ICN remain unclear. In a previous study, the first FRMD7 splice variant (FRMD7‑S) was cloned and identified, and FRMD7‑S was hypothesized to play a significant role in neuronal differentiation and development. The present study investigated a novel multiple exon‑skipping mRNA splice variant of FRMD7, termed FRMD7_SV2, which was detected in NT2 cells using northern blotting. The mRNA expression levels of FRMD7_SV2 in the developing human fetal brain were examined using reverse transcription polymerase chain reaction (PCR), while the expression levels in NT2 cells treated with retinoid acid (RA) or bone morphogenetic protein‑2 were investigated using quantitative PCR. The results revealed that the expression of FRMD7_SV2 was spatially and temporally restricted in human fetal brain development, and was upregulated upon RA‑induced neuronal differentiation of the NT2 cells. These results indicated that as a novel splice variant of FRMD7, FRMD7_SV2 may play a role in neuronal development.
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October 2014
Volume 8 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li Y, Pu J and Zhang B: Expression of a novel splice variant of FRMD7 in developing human fetal brains that is upregulated upon the differentiation of NT2 cells. Exp Ther Med 8: 1131-1136, 2014
APA
Li, Y., Pu, J., & Zhang, B. (2014). Expression of a novel splice variant of FRMD7 in developing human fetal brains that is upregulated upon the differentiation of NT2 cells. Experimental and Therapeutic Medicine, 8, 1131-1136. https://doi.org/10.3892/etm.2014.1916
MLA
Li, Y., Pu, J., Zhang, B."Expression of a novel splice variant of FRMD7 in developing human fetal brains that is upregulated upon the differentiation of NT2 cells". Experimental and Therapeutic Medicine 8.4 (2014): 1131-1136.
Chicago
Li, Y., Pu, J., Zhang, B."Expression of a novel splice variant of FRMD7 in developing human fetal brains that is upregulated upon the differentiation of NT2 cells". Experimental and Therapeutic Medicine 8, no. 4 (2014): 1131-1136. https://doi.org/10.3892/etm.2014.1916