Open Access

Fluorescence resonance energy transfer detection methods: Sensitized emission and acceptor bleaching

  • Authors:
    • Jie Qian
    • Bingbo Yao
    • Chuanyue Wu
  • View Affiliations

  • Published online on: August 22, 2014     https://doi.org/10.3892/etm.2014.1928
  • Pages: 1375-1380
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Abstract

The present study compared the advantages and disadvantages of fluorescence resonance energy transfer (FRET) determination technologies, namely, sensitized emission (SE) and acceptor bleaching (AB), in order to analyze the applicability of SE and AB for studies investigating particularly interesting new cysteine histidine‑rich protein 1 (PINCH1)/integrin‑linked kinase (ILK) interaction. HeLa cells were transfected with cyan fluorescent protein (CFP)‑PINCH1 and yellow fluorescent protein (YFP)‑ILK to establish a PINCH1/ILK interaction examination model. PINCH1/ILK interactions in different parts of the cells were also examined by SE and AB. The FRET determination technologies SE and AB were able to examine PINCH1/ILK interaction. SE was more sensitive for FRET determination and thus had greater reliability. Therefore, SE is highly commended for membrane protein‑protein interaction studies.

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November-2014
Volume 8 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Qian J, Yao B and Wu C: Fluorescence resonance energy transfer detection methods: Sensitized emission and acceptor bleaching. Exp Ther Med 8: 1375-1380, 2014
APA
Qian, J., Yao, B., & Wu, C. (2014). Fluorescence resonance energy transfer detection methods: Sensitized emission and acceptor bleaching. Experimental and Therapeutic Medicine, 8, 1375-1380. https://doi.org/10.3892/etm.2014.1928
MLA
Qian, J., Yao, B., Wu, C."Fluorescence resonance energy transfer detection methods: Sensitized emission and acceptor bleaching". Experimental and Therapeutic Medicine 8.5 (2014): 1375-1380.
Chicago
Qian, J., Yao, B., Wu, C."Fluorescence resonance energy transfer detection methods: Sensitized emission and acceptor bleaching". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1375-1380. https://doi.org/10.3892/etm.2014.1928