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Article

Effect of a heat shock protein 90‑specific inhibitor on the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells

  • Authors:
    • Xue Du
    • Yongmei Li
    • Xu Jing
    • Lina Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China, Department of Microbiology and Immunology, Tianjin Medical University, Tianjin 300052, P.R. China, Department of Biological Engineering, College of Life Sciences, Hebei United University, Tangshan, Hebei 063000, P.R. China
  • Pages: 1559-1564
    |
    Published online on: August 22, 2014
       https://doi.org/10.3892/etm.2014.1930
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Abstract

The aim of the present study was to investigate the effect of heat shock protein 90 (Hsp90)‑specific inhibitor geldanamycin (GA) on the proliferation and apoptosis induced by vascular endothelial growth factor‑C (VEGF‑C) in cervical cancer cells. HeLa cells (1x106/ml) in the logarithmic growth phase were incubated without serum for 24 h. The cells were pretreated with kinase insert domain receptor antibody (KDR)‑Ab (20 µg/ml), phosphatidylinositol 3‑kinase (PI3K) inhibitor LY294002 (3 µmol/l), mitogen‑activated protein kinase (MAPK) inhibitor PD98059 (30 µmol/l) or Hsp90‑specific inhibitor GA (10 µmol/l) for 30 min, and then treated with VEGF‑C (50 ng/µl) for a further 24 h. The cells were harvested for MTT analysis, annexin V‑FITC/propidium iodide double staining for early apoptosis and SDS‑PAGE and western blot analysis in order to determine Hsp90, B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax) and cyclin D1 expression. Treatment with VEGF‑C alone induced Hsp90 protein expression in HeLa cells at all time‑points. Hsp90 expression was increased 3.31‑fold in VEGF‑C treated HeLa cells, and this increase was attenuated in the treatment groups (2.17‑, 1.69‑, 1.82‑fold in VEGF‑C + KDR‑Ab, VEGF‑C + PD98059 and VEGF‑C + LY294002, respectively). The proliferation of the VEGF‑C‑treated HeLa cells was increased ~2.13‑fold, while that of the VEGF‑C + GA‑treated HeLa cells decreased 0.87‑fold (P<0.05). Even low concentrations of GA (0.02 µmol/l) were found to inhibit the Bcl‑2 and cyclin D1 protein expression induced by VEGF‑C. Therefore, the results indicate that the Hsp90‑specific inhibitor GA has a critical role in the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Du X, Li Y, Jing X and Zhao L: Effect of a heat shock protein 90‑specific inhibitor on the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells. Exp Ther Med 8: 1559-1564, 2014.
APA
Du, X., Li, Y., Jing, X., & Zhao, L. (2014). Effect of a heat shock protein 90‑specific inhibitor on the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells. Experimental and Therapeutic Medicine, 8, 1559-1564. https://doi.org/10.3892/etm.2014.1930
MLA
Du, X., Li, Y., Jing, X., Zhao, L."Effect of a heat shock protein 90‑specific inhibitor on the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells". Experimental and Therapeutic Medicine 8.5 (2014): 1559-1564.
Chicago
Du, X., Li, Y., Jing, X., Zhao, L."Effect of a heat shock protein 90‑specific inhibitor on the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1559-1564. https://doi.org/10.3892/etm.2014.1930
Copy and paste a formatted citation
x
Spandidos Publications style
Du X, Li Y, Jing X and Zhao L: Effect of a heat shock protein 90‑specific inhibitor on the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells. Exp Ther Med 8: 1559-1564, 2014.
APA
Du, X., Li, Y., Jing, X., & Zhao, L. (2014). Effect of a heat shock protein 90‑specific inhibitor on the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells. Experimental and Therapeutic Medicine, 8, 1559-1564. https://doi.org/10.3892/etm.2014.1930
MLA
Du, X., Li, Y., Jing, X., Zhao, L."Effect of a heat shock protein 90‑specific inhibitor on the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells". Experimental and Therapeutic Medicine 8.5 (2014): 1559-1564.
Chicago
Du, X., Li, Y., Jing, X., Zhao, L."Effect of a heat shock protein 90‑specific inhibitor on the proliferation and apoptosis induced by VEGF‑C in cervical cancer cells". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1559-1564. https://doi.org/10.3892/etm.2014.1930
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