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Article

Activation of the apoptosis signal‑regulating kinase 1/c‑Jun N‑terminal kinase pathway is involved in the casticin‑induced apoptosis of colon cancer cells

  • Authors:
    • Lin Qu
    • Feng‑Xia Liu
    • Xiao‑Cheng Cao
    • Qiao Xiao
    • Xiaohong Yang
    • Kai‑Qun Ren
  • View Affiliations / Copyright

    Affiliations: Department of Examination, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Laboratory of Medicine, Medical College, Hunan Normal University, Changsha, Hunan 410016, P.R. China
  • Pages: 1494-1500
    |
    Published online on: August 26, 2014
       https://doi.org/10.3892/etm.2014.1934
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Abstract

Casticin is one of the main components of the fruits of Vitex rotundifolia L. Studies have shown that casticin inhibits the growth of various cancer cells, including colon cancer. In the present study, the anti‑carcinogenic effects of casticin on human colon cancer and the underlying mechanisms were investigated. The results revealed that casticin significantly induced apoptosis of HT‑29, HCT‑116, SW480 and Caco‑2 cells, induced the accumulation of reactive oxygen species (ROS) and increased the protein levels of apoptosis signal‑regulating kinase 1 (ASK1), c‑Jun N‑terminal kinase (JNK) and B‑cell lymphoma 2-interacting mediator of cell death (Bim) in HT‑29 cells. Pretreatment with N‑acetylcysteine, an antioxidant chemical compound, inhibited the activation of ASK1, JNK and Bim, as well as the apoptosis induced by casticin. Small interfering RNA targeting ASK1 significantly attenuated the induction of JNK and Bim activation and apoptotic cell death by casticin treatment. SP600125, a specific JNK inhibitor, attenuated Bim activation and apoptosis, but did not alter ASK1 phosphorylation levels. In addition, casticin treatment resulted in apoptosis by the same mechanism in HCT‑116, SW480 and Caco‑2 cells. These results suggest that casticin significantly induced apoptosis by the activation of the ASK1‑JNK‑Bim signaling cascade and the accumulation of ROS in colon cancer cells.
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1 

Sung JJ, Lau JY, Goh KL and Leung WK; Asia Pacific Working Group on Colorectal Cancer. Increasing incidence of colorectal cancer in Asia: implications for screening. Lancet Oncol. 6:871–876. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Xiong F, Wu C, Bi X, et al: Risk of genome-wide association study-identified genetic variants for colorectal cancer in a Chinese population. Cancer Epidemiol Biomarkers Prev. 19:1855–1861. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Laubert T, Habermann JK, Hemmelmann C, et al: Metachronous metastasis- and survival-analysis show prognostic importance of lymphadenectomy for colon carcinomas. BMC Gastroenterol. 12:242012. View Article : Google Scholar : PubMed/NCBI

4 

Lin S, Zhang H, Han T, Wu JZ, Rahman K and Qin LP: In vivo effect of casticin on acute inflammation. Zhong Xi Yi Jie He Xue Bao. 5:573–576. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Li WX, Cui CB, Cai B, Wang HY and Yao XS: Flavonoids from Vitex trifolia L. inhibit cell cycle progression at G2/M phase and induce apoptosis in mammalian cancer cells. J Asian Nat Prod Res. 7:615–626. 2005.

6 

Díaz F, Chávez D, Lee D, et al: Cytotoxic flavone analogues of vitexicarpin, a constituent of the leaves of Vitex negundo. J Nat Prod. 66:865–867. 2003.PubMed/NCBI

7 

Haïdara K, Zamir L, Shi QW and Batist G: The flavonoid Casticin has multiple mechanisms of tumor cytotoxicity action. Cancer Lett. 242:180–190. 2006.PubMed/NCBI

8 

Imai M, Kikuchi H, Denda T, Ohyama K, Hirobe C and Toyoda H: Cytotoxic effects of flavonoids against a human colon cancer derived cell line, COLO 201: a potential natural anti-cancer substance. Cancer Lett. 276:74–80. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Chen D, Cao J, Tian L, Liu F and Sheng X: Induction of apoptosis by casticin in cervical cancer cells through reactive oxygen species-mediated mitochondrial signaling pathways. Oncol Rep. 26:1287–1294. 2011.PubMed/NCBI

10 

Yang J, Yang Y, Tian L, Sheng XF, Liu F and Cao JG: Casticin-induced apoptosis involves death receptor 5 upregulation in hepatocellular carcinoma cells. World J Gastroenterol. 17:4298–4307. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Zeng F, Tian L, Liu F, Cao J, Quan M and Sheng X: Induction of apoptosis by casticin in cervical cancer cells: reactive oxygen species-dependent sustained activation of Jun N-terminal kinase. Acta Biochim Biophys Sin (Shanghai). 44:442–449. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Gamaley IA and Klyubin IV: Roles of reactive oxygen species: signaling and regulation of cellular functions. Int Rev Cytol. 188:203–255. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Xiao D, Powolny AA and Singh SV: Benzyl isothiocyanate targets mitochondrial respiratory chain to trigger reactive oxygen species-dependent apoptosis in human breast cancer cells. J Biol Chem. 283:30151–30163. 2008. View Article : Google Scholar

14 

Boveris A: Mitochondrial production of superoxide radical and hydrogen peroxide. Adv Exp Med Biol. 78:67–82. 1977. View Article : Google Scholar : PubMed/NCBI

15 

Kanamoto T, Mota M, Takeda K, et al: Role of apoptosis signal-regulating kinase in regulation of the c-Jun N-terminal kinase pathway and apoptosis in sympathetic neurons. Mol Cell Biol. 20:196–204. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Gotoh Y and Cooper JA: Reactive oxygen species- and dimerization-induced activation of apoptosis signal-regulating kinase 1 in tumor necrosis factor-alpha signal transduction. J Biol Chem. 273:17477–17482. 1998. View Article : Google Scholar

17 

Nishitoh H, Matsuzawa A, Tobiume K, et al: ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeats. Genes Dev. 16:1345–1355. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Puthalakath H, Huang DC, O’Reilly LA, King SM and Strasser A: The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex. Mol Cell. 3:287–296. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Strasser A, Puthalakath H, Bouillet P, et al: The role of bim, a proapoptotic BH3-only member of the Bcl-2 family in cell-death control. Ann NY Acad Sci. 917:541–548. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Song JJ, Rhee JG, Suntharalingam M, Walsh SA, Spitz DR and Lee YJ: Role of glutaredoxin in metabolic oxidative stress. Glutaredoxin as a sensor of oxidative stress mediated by H2O2. J Biol Chem. 277:46566–46575. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Yang XH, Zheng X, Cao JG, Xiang HL, Liu F and Lv Y: 8-Bromo-7-methoxychrysin-induced apoptosis of hepatocellular carcinoma cells involves ROS and JNK. World J Gastroenterol. 16:3385–3393. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Zhao XC, Tian L, Cao JG and Liu F: Induction of apoptosis by 5,7-dihydroxy-8-nitrochrysin in breast cancer cells: the role of reactive oxygen species and Akt. Int J Oncol. 37:1345–1352. 2010.PubMed/NCBI

23 

Lee BC, Park BH, Kim SY and Lee YJ: Role of Bim in diallyl trisulfide-induced cytotoxicity in human cancer cells. J Cell Biochem. 112:118–127. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Ohyama K, Akaike T, Hirobe C and Yamakawa T: Cytotoxicity and apoptotic inducibility of Vitex agnus-castus fruit extract in cultured human normal and cancer cells and effect on growth. Biol Pharm Bull. 26:10–18. 2003.PubMed/NCBI

25 

Shen JK, Du HP, Yang M, Wang YG and Jin J: Casticin induces leukemic cell death through apoptosis and mitotic catastrophe. Ann Hematol. 88:743–752. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Jing Y, Dai J, Chalmers-Redman RM, Tatton WG and Waxman S: Arsenic trioxide selectively induces acute promyelocytic leukemia cell apoptosis via a hydrogen peroxide-dependent pathway. Blood. 94:2102–2111. 1999.PubMed/NCBI

27 

Matsuzawa A, Saegusa K, Noguchi T, et al: ROS-dependent activation of the TRAF6-ASK1-p38 pathway is selectively required for TLR4-mediated innate immunity. Nat Immunol. 6:587–592. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Tobiume K, Saitoh M and Ichijo H: Activation of apoptosis signal-regulating kinase 1 by the stress-induced activating phosphorylation of pre-formed oligomer. J Cell Physiol. 191:95–104. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Matsuzawa A and Ichijo H: Redox control of cell fate by MAP kinase: physiological roles of ASK1-MAP kinase pathway in stress signaling. Biochim Biophys Acta. 1780:1325–1336. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Jin HO, Park IC, An S, et al: Up-regulation of Bak and Bim via JNK downstream pathway in the response to nitric oxide in human glioblastoma cells. J Cell Physiol. 206:477–486. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Kuo CT, Hsu MJ, et al: Denbinobin induces apoptosis in human lung adenocarcinoma cells via Akt inactivation, Bad activation, and mitochondrial dysfunction. Toxicol Lett. 177:48–58. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Qu L, Liu FX, Cao XC, Xiao Q, Yang X and Ren KQ: Activation of the apoptosis signal‑regulating kinase 1/c‑Jun N‑terminal kinase pathway is involved in the casticin‑induced apoptosis of colon cancer cells. Exp Ther Med 8: 1494-1500, 2014.
APA
Qu, L., Liu, F., Cao, X., Xiao, Q., Yang, X., & Ren, K. (2014). Activation of the apoptosis signal‑regulating kinase 1/c‑Jun N‑terminal kinase pathway is involved in the casticin‑induced apoptosis of colon cancer cells. Experimental and Therapeutic Medicine, 8, 1494-1500. https://doi.org/10.3892/etm.2014.1934
MLA
Qu, L., Liu, F., Cao, X., Xiao, Q., Yang, X., Ren, K."Activation of the apoptosis signal‑regulating kinase 1/c‑Jun N‑terminal kinase pathway is involved in the casticin‑induced apoptosis of colon cancer cells". Experimental and Therapeutic Medicine 8.5 (2014): 1494-1500.
Chicago
Qu, L., Liu, F., Cao, X., Xiao, Q., Yang, X., Ren, K."Activation of the apoptosis signal‑regulating kinase 1/c‑Jun N‑terminal kinase pathway is involved in the casticin‑induced apoptosis of colon cancer cells". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1494-1500. https://doi.org/10.3892/etm.2014.1934
Copy and paste a formatted citation
x
Spandidos Publications style
Qu L, Liu FX, Cao XC, Xiao Q, Yang X and Ren KQ: Activation of the apoptosis signal‑regulating kinase 1/c‑Jun N‑terminal kinase pathway is involved in the casticin‑induced apoptosis of colon cancer cells. Exp Ther Med 8: 1494-1500, 2014.
APA
Qu, L., Liu, F., Cao, X., Xiao, Q., Yang, X., & Ren, K. (2014). Activation of the apoptosis signal‑regulating kinase 1/c‑Jun N‑terminal kinase pathway is involved in the casticin‑induced apoptosis of colon cancer cells. Experimental and Therapeutic Medicine, 8, 1494-1500. https://doi.org/10.3892/etm.2014.1934
MLA
Qu, L., Liu, F., Cao, X., Xiao, Q., Yang, X., Ren, K."Activation of the apoptosis signal‑regulating kinase 1/c‑Jun N‑terminal kinase pathway is involved in the casticin‑induced apoptosis of colon cancer cells". Experimental and Therapeutic Medicine 8.5 (2014): 1494-1500.
Chicago
Qu, L., Liu, F., Cao, X., Xiao, Q., Yang, X., Ren, K."Activation of the apoptosis signal‑regulating kinase 1/c‑Jun N‑terminal kinase pathway is involved in the casticin‑induced apoptosis of colon cancer cells". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1494-1500. https://doi.org/10.3892/etm.2014.1934
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