Overexpression of CyclinA2 ameliorates hypoxia‑impaired proliferation of cardiomyocytes

Deng,Huiling; Cheng,Yong; Guo,Zhikun; Zhang,Fenxi; Lu,Xing; Feng,Lingling; Wang,Xianwei; Xu,Zhenping

doi:10.3892/etm.2014.1935

November-2014 Volume 8 Issue 5

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November-2014 Volume 8 Issue 5

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Article

Overexpression of CyclinA2 ameliorates hypoxia‑impaired proliferation of cardiomyocytes

Authors:
- Huiling Deng
- Yong Cheng
- Zhikun Guo
- Fenxi Zhang
- Xing Lu
- Lingling Feng
- Xianwei Wang
- Zhenping Xu
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Affiliations: Department of Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Key Laboratory of Henan Province for Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Anatomy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Pages: 1513-1517
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Published online on: August 26, 2014

https://doi.org/10.3892/etm.2014.1935
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Abstract

Hypoxia is a primary mediator for cell survival, and has been reported to inhibit cardiomyocyte proliferation in fetal and neonatal hearts. CyclinA2 is a key regulator of cell proliferation. Whether CyclinA2 affects cardiomyocyte proliferation in hypoxic conditions remains unexamined. This study was designed to investigate the roles of CyclinA2 expression on hypoxia‑impaired cardiomyocyte proliferation. Cardiomyocytes were isolated from neonatal rats and randomly separated into six groups: Control, hypoxia, enhanced green fluorescent protein (EGFP)‑Adv, EGFP‑Ccna2, EGFP‑Adv + hypoxia and EGFP‑Ccna2 + hypoxia. The cells in the control group were cultured in a general cell incubator; the cells in the hypoxia group were placed in a hypoxic chamber for 12 h; the cells in the EGFP‑Adv and EGFP‑Ccna2 groups were separately transfected with EGFP‑adenovirus capsids or EGFP‑adenovirus capsids with CyclinA2 cDNA for 18 h, and then placed in a general incubator for an additional 12 h; the cells in the EGFP‑Adv + hypoxia and EGFP‑Ccna2 + hypoxia groups were separately transfected with EGFP‑adenovirus capsids or EGFP‑adenovirus capsids with CyclinA2 cDNA for 18 h, and then placed in a hypoxia chamber for an additional 12 h. CyclinA2 expression was measured using immunochemical staining and western blot analysis, and cardiomyocyte proliferation was measured using the cell counting kit 8. GFP fluorescence indicated a high transfection efficiency (>80%), and immunochemical staining showed that CyclinA2 was mainly distributed in the nucleus. CyclinA2 expression was downregulated following exposure to hypoxia for 12 h. Cardiomyocyte proliferation was also significantly decreased following exposure to hypoxia for 12 h. However, compared with the EGFP‑Adv group, CyclinA2 expression and cardiomyocyte proliferation was markedly increased in the EGFP‑Ccna2 group. Furthermore, compared with the EGFP‑Adv + hypoxia group, CyclinA2 expression and cell proliferation were markedly increased in the EGFP‑Ccna2 + hypoxia group. These findings indicate that CyclinA2 upregulation improves cardiomyocyte proliferation in hypoxic conditions.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Overexpression of CyclinA2 ameliorates hypoxia‑impaired proliferation of cardiomyocytes

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