Evaluation of blood‑brain barrier permeability in tryptophan hydroxylase 2‑knockout mice

Xu,Chao‑Jin; Zhang,Peng; Dai,Ting‑Li; Niu,Xue‑Yuan; Wang,Jun‑Ling; Jin,Ming‑Shun; Liao,Min

doi:10.3892/etm.2014.1938

November-2014 Volume 8 Issue 5

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November-2014 Volume 8 Issue 5

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Article

Evaluation of blood‑brain barrier permeability in tryptophan hydroxylase 2‑knockout mice

Authors:
- Chao‑Jin Xu
- Peng Zhang
- Ting‑Li Dai
- Xue‑Yuan Niu
- Jun‑Ling Wang
- Ming‑Shun Jin
- Min Liao
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Affiliations: Department of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Department of Anatomy, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China
Pages: 1467-1470
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Published online on: August 28, 2014

https://doi.org/10.3892/etm.2014.1938
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Abstract

The blood‑brain barrier (BBB) is critical to the health of the central nervous system (CNS). The possibility that 5‑hydroxytryptamine (5‑HT) participates in the alteration of the BBB has been previously demonstrated. Tryptophan hydroxylase 2 (TPH2) is a unique genetic enzyme isoform that catalyzes the rate‑limiting step in the biosynthesis of 5‑HT in the CNS; however, its role in the permeability changes of the BBB remains unclear. In the present study, TPH2‑knockout mice were utilized in the assessment of BBB disruption, as measured by the Evans Blue (EB) extravasation or fluorescein isothiocyanate‑albumin leakage assay in the brain. EB was not found to be retained in the brain in the TPH2‑knockout mice or the wild‑type controls. The results of the study demonstrate that TPH2 knockout has no effect on BBB permeability, indicating that TPH2 and the 5‑HT system in the CNS are not sufficient to influence the BBB leakage.

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