Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2014 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2014 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Cellular and molecular mechanism study of declined intestinal transit function in the cholesterol gallstone formation process of the guinea pig

  • Authors:
    • Ying Fan
    • Shuodong Wu
    • Zhenhua Yin
    • Bei‑Bei Fu
  • View Affiliations / Copyright

    Affiliations: Department of the Second General Surgery, Sheng Jing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 1518-1522
    |
    Published online on: September 1, 2014
       https://doi.org/10.3892/etm.2014.1943
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to investigate the cellular and molecular mechanisms of declined intestinal transit (IT) function in the cholesterol gallstone (CG) formation process. Forty guinea pigs were divided into an experimental group (EG) and a control group (CoG), and the reverse transcription‑polymerase chain reaction (RT‑PCR) was performed for the analysis of c‑kit and stem cell factor (scf) mRNA expression in the small bowel. In addition, immunofluorescence staining and confocal laser microscopy were performed for the observation of the changes in the number of interstitial cells of Cajal (ICCs) in the terminal ileum of each group. RT‑PCR showed that, compared with the CoG, the intestinal c‑kit and scf mRNA expression levels in the EG were significantly decreased; the average positive area of ICCs in the ileum in the EG was also significantly reduced. During the diet‑induced CG formation procedure, the c‑kit and scf mRNA expression levels in the small intestine decreased and the number of ICCs decreased. Inhibition of the c‑kit/scf pathway may be involved in the declined IT function during the CG formation process.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Hussaini SH, Pereira SP, Dowling RH and Wass JA: Slow intestinal transit and gallstone formation. Lancet. 341:6381993. View Article : Google Scholar : PubMed/NCBI

2 

Spathis A, Heaton KW, Emmett PM, Norboo T and Hunt L: Gallstones in a community free of obesity but prone to slow intestinal transit. Eur J Gastroenterol Hepatol. 9:201–206. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Fan Y, Wu SD and Fu BB: Effect of intestinal transit on the formation of cholesterol gallstones in hamsters. Hepatobiliary Pancreat Dis Int. 6:513–515. 2007.PubMed/NCBI

4 

Jun JY: The important roles of interstitial cells of cajal and cholinergic receptors on diabetes related dysfunction of colon. J Neurogastroenterol Motil. 17:333–334. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Iino S, Horiguchi S and Horiguchi K: Interstitial cells of Cajal in the gastrointestinal musculature of W(jic) c-kit mutant mice. J Smooth Muscle Res. 47:111–121. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Cole WC: ANO1-ther brick in the wall - role of Ca2+-activated Cl- channels of interstitial cells of Cajal in cholinergic motor control of gastrointestinal smooth muscle. J Physiol. 589:4641–4642. 2011.PubMed/NCBI

7 

Tanaka C, Kaji H, He J, et al: Rab27b regulates c-kit expression by controlling the secretion of stem cell factor. Biochem Biophys Res Commun. 419:368–373. 2012. View Article : Google Scholar : PubMed/NCBI

8 

He X, Yang WC, Wen XY, et al: Late embryonic and postnatal development of interstitial cells of cajal in mouse esophagus: distribution, proliferation and kit dependence. Cells Tissues Organs. 196:175–188. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Portincasa P, Di Ciaula A, Wang HH, et al: Coordinate regulation of gallbladder motor function in the gut-liver axis. Hepatology. 47:2112–2116. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Xu QW, Scott RB, Tan DT and Shaffer EA: Slow intestinal transit: a motor disorder contributing to cholesterol gallstone formation in the ground squirrel. Hepatology. 23:1664–1672. 1996. View Article : Google Scholar : PubMed/NCBI

11 

Wang DQ, Cohen DE and Carey MC: Biliary lipids and cholesterol gallstone disease. J Lipid Res. 50(Suppl): S406–S411. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Zhang ZH, Wu SD, Su Y, et al: Differences and significance of motilin, vasoactive intestinal peptide and gastrin in blood and gallbladder tissues of patients with gallstones. Hepatobiliary Pancreat Dis Int. 7:58–64. 2008.PubMed/NCBI

13 

Xie M, Kotecha VR, Andrade JD, Fox JG and Carey MC: Augmented cholesterol absorption and sarcolemmal sterol enrichment slow small intestinal transit in mice, contributing to cholesterol cholelithogenesis. J Physiol. 590:1811–1824. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Torihashi S, Horisawa M and Watanabe Y: c-Kit immunoreactive interstitial cells in the human gastrointestinal tract. J Auton Nerv Syst. 75:38–50. 1999. View Article : Google Scholar : PubMed/NCBI

15 

McCann CJ, Hwang SJ, Bayguinov Y, Colletti EJ, Sanders KM and Ward SM: Establishment of pacemaker activity in tissues allotransplanted with interstitial cells of Cajal. Neurogastroenterol Motil. 25:e418–e428. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Neuhann TM, Mansmann V, Merkelbach-Bruse S, et al: A novel germline KIT mutation (p. L576P) in a family presenting with juvenile onset of multiple gastrointestinal stromal tumors, skin hyperpigmentations, and esophageal stenosis. Am J Surg Pathol. 37:898–905. 2013. View Article : Google Scholar

17 

Hebert MD: Signals controlling Cajal body assembly and function. Int J Biochem Cell Biol. 45:1314–1317. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Lee JH, Kim SY, Kwon YK, Kim BJ and So I: Characteristics of the cholecystokinin-induced depolarization of pacemaking activity in cultured interstitial cells of Cajal from murine small intestine. Cell Physiol Biochem. 31:542–554. 2013. View Article : Google Scholar

19 

Albertí E, Mikkelsen HB, Wang XY, et al: Pacemaker activity and inhibitory neurotrans- mission in the colon of Ws/Ws mutant rats. Am J Physiol Gastrointest Liver Physiol. 292:G1499–G1510. 2007.

20 

Torihashi S, Ward SM, Nishikawa S, et al: c-kit-dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract. Cell Tissue Res. 280:97–111. 1995.PubMed/NCBI

21 

Chen J, Du L, Xiao YT and Cai W: Disruption of interstitial cells of Cajal networks after massive small bowel resection. World J Gastroenterol. 19:3415–3422. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Wixner J, Obayashi K, Ando Y, Karling P and Anan I: Loss of gastric interstitial cells of Cajal in patients with hereditary transthyretin amyloidosis. Amyloid. 20:99–106. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Mogami S, Suzuki H, Tsugawa H, Fukuhara S and Hibi T: Impaired heme oxygenase-1 induction in the gastric antrum induces disruption of the interstitial cells of Cajal network in a rat model of streptozotocin-induced diabetes. Neurogastroenterol Motil. 25:609–e465. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Li F, Zhang L, Li C, et al: Adenovirus-mediated stem cell leukemia gene transfer induces rescue of interstitial cells of Cajal in ICC-loss mice. Int J Colorectal Dis. 25:557–566. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Mikkelsen HB, Malysz J, Huizinga JD and Thuneberg L: Action potential generation, Kit receptor immunohistochemistry and morphology of steel-Dickie (Sl/Sld) mutant mouse small intestine. Neurogastroenterol Motil. 10:11–26. 1998. View Article : Google Scholar

26 

Rich A, Miller SM, Gibbons SJ, Malysz J, Szurszewski JH and Farrugia G: Local presentation of Steel factor increases expression of c-kit immunoreactive interstitial cells of Cajal in culture. Am J Physiol Gastrointest Liver Physiol. 284:G313–G320. 2003.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Fan Y, Wu S, Yin Z and Fu BB: Cellular and molecular mechanism study of declined intestinal transit function in the cholesterol gallstone formation process of the guinea pig. Exp Ther Med 8: 1518-1522, 2014.
APA
Fan, Y., Wu, S., Yin, Z., & Fu, B. (2014). Cellular and molecular mechanism study of declined intestinal transit function in the cholesterol gallstone formation process of the guinea pig. Experimental and Therapeutic Medicine, 8, 1518-1522. https://doi.org/10.3892/etm.2014.1943
MLA
Fan, Y., Wu, S., Yin, Z., Fu, B."Cellular and molecular mechanism study of declined intestinal transit function in the cholesterol gallstone formation process of the guinea pig". Experimental and Therapeutic Medicine 8.5 (2014): 1518-1522.
Chicago
Fan, Y., Wu, S., Yin, Z., Fu, B."Cellular and molecular mechanism study of declined intestinal transit function in the cholesterol gallstone formation process of the guinea pig". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1518-1522. https://doi.org/10.3892/etm.2014.1943
Copy and paste a formatted citation
x
Spandidos Publications style
Fan Y, Wu S, Yin Z and Fu BB: Cellular and molecular mechanism study of declined intestinal transit function in the cholesterol gallstone formation process of the guinea pig. Exp Ther Med 8: 1518-1522, 2014.
APA
Fan, Y., Wu, S., Yin, Z., & Fu, B. (2014). Cellular and molecular mechanism study of declined intestinal transit function in the cholesterol gallstone formation process of the guinea pig. Experimental and Therapeutic Medicine, 8, 1518-1522. https://doi.org/10.3892/etm.2014.1943
MLA
Fan, Y., Wu, S., Yin, Z., Fu, B."Cellular and molecular mechanism study of declined intestinal transit function in the cholesterol gallstone formation process of the guinea pig". Experimental and Therapeutic Medicine 8.5 (2014): 1518-1522.
Chicago
Fan, Y., Wu, S., Yin, Z., Fu, B."Cellular and molecular mechanism study of declined intestinal transit function in the cholesterol gallstone formation process of the guinea pig". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1518-1522. https://doi.org/10.3892/etm.2014.1943
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team