Levels of microRNA‑181b and plasminogen activator inhibitor‑1 are associated with hypertensive disorders complicating pregnancy

Chen,Yan‑Shan; Shen,Ling; Mai,Rui‑Qin; Wang,Ying

doi:10.3892/etm.2014.1946

Levels of microRNA‑181b and plasminogen activator inhibitor‑1 are associated with hypertensive disorders complicating pregnancy

Authors:
- Yan‑Shan Chen
- Ling Shen
- Rui‑Qin Mai
- Ying Wang
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Affiliations: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, P.R. China, Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, P.R. China
Published online on: September 2, 2014 https://doi.org/10.3892/etm.2014.1946
Pages: 1523-1527

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Abstract

The aim of the present study was to explore the association between the expression of microRNA (miRNA)‑181b and plasminogen activator inhibitor‑1 (PAI‑1) in the placental tissue of pregnant females with a hypertensive disorder complicating pregnancy (HDCP). Placental tissue samples were obtained from 48 patients with HDCP and 40 females with a normal pregnancy. The levels of miRNA‑181b and PAI‑1 mRNA were determined by the reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The expression of PAI‑1 protein was analyzed by western blotting. Vascular smooth muscle cells (VSMCs) were transfected with the pEGP‑miRNA‑181b plasmid using Lipofectamine® 2000. Transfection efficiency was confirmed by immunohistochemical analysis. The levels of miRNA‑181b in the placental tissue of patients with HDCP were lower than those in the control group, whereas the levels of PAI‑1 mRNA in the placental tissue of patients with HDCP were higher than those in the control group. The expression of the PAI‑1 protein in the HDCP group was higher than that in the control group. Following transfection of VSMCs with plasmid pGCMV/EGFP/miRNA‑181b, the levels of PAI‑1 mRNA were reduced while the levels of miRNA‑181 were upregulated. Furthermore, the expression levels of PAI‑1 protein were lower than those in the control group. The levels of miRNA‑181b and PAI‑1 mRNA were strongly associated with HDCP. Thus, miRNA‑181b may play an important role in the regulation of PAI‑1. PAI‑1 and miRNA‑181b may be novel biomarkers to be used in HDCP therapy.

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1	Zhou J, Xiao XM and Wu YH: Expression of interferon-γ in decidual natural killer cells from women with hypertensive disorder complicating pregnancy. J Obstet Gynaecol Res. 40:670–676. 2014.
2	Yang L, Wang YL, Liu S, et al: miR-181b promotes cell proliferation and reduces apoptosis by repressing the expression of adenylyl cyclase 9 (AC9) in cervical cancer cells. FEBS Lett. 588:124–130. 2014. View Article : Google Scholar : PubMed/NCBI
3	Shi L, Cheng Z, Zhang J, et al: hsa-mir-181a and hsa-mir-181b function as tumor suppressors in human glioma cells. Brain Res. 1236:185–193. 2008. View Article : Google Scholar : PubMed/NCBI
4	Simone TM, Archambeault J and Higgins PJ: Small molecule targeting of PAI-1 function: a new therapeutic approach for treatment of vascular stenosis. J Mol Genet Med. 7:10000592013. View Article : Google Scholar : PubMed/NCBI
5	Gomes-Giacoia E, Miyake M, Goodison S and Rosser CJ: Targeting plasminogen activator inhibitor-1 inhibits angiogenesis and tumor growth in a human cancer xenograft model. Mol Cancer Ther. 12:2697–2708. 2013. View Article : Google Scholar
6	Boe AE, Eren M, Murphy SB, et al: Plasminogen activator inhibitor-1 antagonist TM5441 attenuates Nω-nitro-L-arginine methyl ester-induced hypertension and vascular senescence. Circulation. 128:2318–2324. 2013.PubMed/NCBI
7	Li LX, Liu YL, Wen JG, et al: Expression and significance of aquaporin 1 in placenta, placental membranes and peritoneum of patients with hypertensive disorder complicating pregnancy. Zhonghua Fu Chan Ke Za Zhi. 43:497–501. 2008.(In Chinese).
8	Salmani D, Purushothaman S, Somashekara SC, et al: Study of structural changes in placenta in pregnancy-induced hypertension. J Nat Sci Biol Med. 5:352–355. 2014. View Article : Google Scholar : PubMed/NCBI
9	Okawara M, Seki H, Matsuoka K, et al: Examination of the expression of cyclooxygenase-2 in placenta villi from sufferers of pregnancy induced hypertension. Biol Pharm Bull. 32:2053–2056. 2009. View Article : Google Scholar : PubMed/NCBI
10	Henao-Mejia J, Williams A, Goff LA, et al: The microRNA miR-181 is a critical cellular metabolic rheostat essential for NKT cell ontogenesis and lymphocyte development and homeostasis. Immunity. 38:984–997. 2013. View Article : Google Scholar : PubMed/NCBI
11	Zhu Y, Wu J, Li S, et al: The function role of miR-181a in chemosensitivity to adriamycin by targeting Bcl-2 in low-invasive breast cancer cells. Cell Physiol Biochem. 32:1225–1237. 2013. View Article : Google Scholar : PubMed/NCBI
12	Zhai XF, Fang FF, Liu Q, et al: MiR-181a contributes to bufalin-induced apoptosis in PC-3 prostate cancer cells. BMC Complement Altern Med. 13:3252013. View Article : Google Scholar : PubMed/NCBI
13	Wang XF, Shi ZM, Wang XR, et al: MiR-181d acts as a tumor suppressor in glioma by targeting K-ras and Bcl-2. J Cancer Res Clin Oncol. 138:573–584. 2012. View Article : Google Scholar : PubMed/NCBI
14	Kronski E, Fiori ME, Barbieri O, et al: miR181b is induced by the chemopreventive polyphenol curcumin and inhibits breast cancer metastasis via down-regulation of the inflammatory cytokines CXCL1 and -2. Mol Oncol. 8:581–595. 2014. View Article : Google Scholar : PubMed/NCBI
15	Yan JJ, Zhu XM, Yin GW, et al: Effect of human miR-181a on function of trophoblast cell line HTR-8/SVneo. Zhonghua Lin Chuang Yi Shi Za Zhi (Dian Zi Ban). 7:136–138. 2013.(In Chinese).
16	Simone TM and Higgins PJ: Low molecular weight antagonists of plasminogen activator inhibitor-1: therapeutic potential in cardiovascular disease. Mol Med Ther. 1:1012012. View Article : Google Scholar : PubMed/NCBI
17	Yang Z, Wan X, Gu Z, et al: Evolution of the mir-181 microRNA family. Comput Biol Med. 52:82–87. 2014. View Article : Google Scholar : PubMed/NCBI
18	Madonna R and De Caterina R: Cellular and molecular mechanisms of vascular injury in diabetes - part I: pathways of vascular disease in diabetes. Vascul Pharmacol. 54:68–74. 2011. View Article : Google Scholar : PubMed/NCBI
19	Cawyer RC, Horvat D, Leonard D, et al: Hyperglycemia impairs cytotrophoblast function via stress signaling. Am J Obstet Gynecol. May 1–2014.(Epub ahead of print).
20	Su L, Liu R, Cheng W, et al: Expression patterns of microRNAs in porcine endometrium and their potential roles in embryo implantation and placentation. PLoS One. 9:e878672014. View Article : Google Scholar : PubMed/NCBI
21	Estella C, Herrer I, Moreno-Moya JM, et al: miRNA signature and Dicer requirement during human endometrial stromal decidualization in vitro. PLoS One. 7:e410802012. View Article : Google Scholar : PubMed/NCBI