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Article

Neferine inhibits angiotensin II‑induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression

  • Authors:
    • Lulu Zheng
    • Yongwen Cao
    • Shao Liu
    • Zhenyu Peng
    • Saidan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Emergency, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 1545-1550
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    Published online on: September 9, 2014
       https://doi.org/10.3892/etm.2014.1952
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Abstract

Neferine inhibits the angiotensin II (AngII)‑induced proliferation of vascular smooth muscle cells (SMCs), but the underlying mechanism is unclear. The aim of this study was to explore the mechanism underlying the effect of neferine on the proliferation of vascular SMCs. Rat aortic SMCs (RASMCs) were used and fractalkine (Fkn) gene expression was measured by quantitative polymerase chain reaction and western blot analysis. The proliferation of RASMCs was analyzed by MTT assay and flow cytometry. It was revealed that AngII induced Fkn expression in a dose‑ and time‑dependent manner. Fkn‑knockdown with small interfering RNA attenuated the AngII‑induced RASMC proliferation. Furthermore, neferine inhibited Fkn expression and attenuated the AngII‑induced RASMC proliferation. These findings suggest that the Fkn gene may play an important role in AngII‑induced RASMC proliferation and that neferine acts to attenuate AngII‑induced RASMC proliferation by inhibiting Fkn expression.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng L, Cao Y, Liu S, Peng Z and Zhang S: Neferine inhibits angiotensin II‑induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression. Exp Ther Med 8: 1545-1550, 2014.
APA
Zheng, L., Cao, Y., Liu, S., Peng, Z., & Zhang, S. (2014). Neferine inhibits angiotensin II‑induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression. Experimental and Therapeutic Medicine, 8, 1545-1550. https://doi.org/10.3892/etm.2014.1952
MLA
Zheng, L., Cao, Y., Liu, S., Peng, Z., Zhang, S."Neferine inhibits angiotensin II‑induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression". Experimental and Therapeutic Medicine 8.5 (2014): 1545-1550.
Chicago
Zheng, L., Cao, Y., Liu, S., Peng, Z., Zhang, S."Neferine inhibits angiotensin II‑induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1545-1550. https://doi.org/10.3892/etm.2014.1952
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng L, Cao Y, Liu S, Peng Z and Zhang S: Neferine inhibits angiotensin II‑induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression. Exp Ther Med 8: 1545-1550, 2014.
APA
Zheng, L., Cao, Y., Liu, S., Peng, Z., & Zhang, S. (2014). Neferine inhibits angiotensin II‑induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression. Experimental and Therapeutic Medicine, 8, 1545-1550. https://doi.org/10.3892/etm.2014.1952
MLA
Zheng, L., Cao, Y., Liu, S., Peng, Z., Zhang, S."Neferine inhibits angiotensin II‑induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression". Experimental and Therapeutic Medicine 8.5 (2014): 1545-1550.
Chicago
Zheng, L., Cao, Y., Liu, S., Peng, Z., Zhang, S."Neferine inhibits angiotensin II‑induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1545-1550. https://doi.org/10.3892/etm.2014.1952
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