Asialoerythropoietin ameliorates bleomycin‑induced acute lung injury in rabbits by reducing inflammation

Sonoda,Akinaga; Nitta,Norihisa; Tsuchiya,Keiko; Otani,Hideji; Watanabe,Shobu; Mukaisho,Kenichi; Tomozawa,Yuki; Nagatani,Yukihiro; Ohta,Shinichi; Takahashi,Masashi; Murata,Kiyoshi

doi:10.3892/etm.2014.1960

November-2014 Volume 8 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

November-2014 Volume 8 Issue 5

Full Size Image

Article

Asialoerythropoietin ameliorates bleomycin‑induced acute lung injury in rabbits by reducing inflammation

Authors:
- Akinaga Sonoda
- Norihisa Nitta
- Keiko Tsuchiya
- Hideji Otani
- Shobu Watanabe
- Kenichi Mukaisho
- Yuki Tomozawa
- Yukihiro Nagatani
- Shinichi Ohta
- Masashi Takahashi
- Kiyoshi Murata
View Affiliations / Copyright

Affiliations: Department of Radiology, Shiga University of Medical Science, Otsu, Shiga 520‑2192, Japan, Department of Pathology, Shiga University of Medical Science, Otsu, Shiga 520‑2192, Japan
Pages: 1443-1446
|
Published online on: September 12, 2014

https://doi.org/10.3892/etm.2014.1960
Expand metrics +

Abstract

Acute lung injury, a critical illness characterized by acute respiratory failure with bilateral pulmonary infiltrates, remains unresponsive to current treatments. The condition involves injury to the alveolar capillary barrier, neutrophil accumulation and the induction of proinflammatory cytokines followed by lung fibrosis. In the present study, a rabbit model of bleomycin‑induced acute lung injury was established to examine the effects of asialoerythropoietin (AEP), an agent with tissue‑protective activities, on pulmonary inflammation. Six Japanese white rabbits were randomly divided into two equal groups. Acute lung injury was induced in all rabbits by intratracheally injecting bleomycin. The control group was injected with bleomycin only; the experimental (AEP) group was injected intravenously with AEP (80 µg/kg) prior to the bleomycin injection. Computed tomography (CT) studies were performed seven days later. The CT inflammatory scores of areas exhibiting abnormal density and the pathological inflammatory scores were recorded as a ratio on a 7x7 mm grid. The CT and pathological inflammatory scores were significantly different between the control and AEP groups [122±10 and 16.3±1.5 (controls) vs. 71±8.5 and 9.7±1.4 (AEP), respectively; P<0.01]. Thus, the present study revealed that AEP prevents bleomycin‑induced acute lung injury in rabbits.

View Figures

View References

1	Saguil A and Fargo M: Acute respiratory distress syndrome: diagnosis and management. Am Fam Physician. 85:352–358. 2012.
2	Cortés I, Peñuelas O and Esteban A: Acute respiratory distress syndrome: evaluation and management. Minerva Anestesiol. 78:343–357. 2012.
3	Brun-Buisson C, Minelli C, Bertolini G, et al: Epidemiology and outcome of acute lung injury in European intensive care units. Results from the ALIVE study. Intensive Care Med. 30:51–61. 2004. View Article : Google Scholar : PubMed/NCBI
4	Ware LB: Clinical year in review IV: Acute respiratory distress syndrome, radiology in the intensive care unit, nonpulmonary critical care, and pulmonary infections in the immunocompromised host. Proc Am Thorac Soc. 5:755–760. 2008.PubMed/NCBI
5	Mofidi A, Bader A and Pavlica S: The use of erythropoietin and its derivatives to treat spinal cord injury. Mini Rev Med Chem. 11:763–770. 2011. View Article : Google Scholar : PubMed/NCBI
6	Rjiba-Touati K, Ayed-Boussema I, Belarbia A, Achour A and Bacha H: Recombinant human erythropoietin prevents cisplatin-induced genotoxicity in rat liver and heart tissues via an antioxidant process. Drug Chem Toxicol. 35:134–140. 2012. View Article : Google Scholar
7	Watanabe D, Suzuma K, Matsui S, et al: Erythropoietin as a retinal angiogenic factor in proliferative diabetic retinopathy. N Engl J Med. 353:782–792. 2005. View Article : Google Scholar : PubMed/NCBI
8	Shang Y, Wu Y, Yao S, et al: Protective effect of erythropoietin against ketamine-induced apoptosis in cultured rat cortical neurons: involvement of PI3K/Akt and GSK-3 beta pathway. Apoptosis. 12:2187–2195. 2007. View Article : Google Scholar : PubMed/NCBI
9	Wang W, Horner DN, Chen WL, Zandstra PW and Audet J: Synergy between erythropoietin and stem cell factor during erythropoiesis can be quantitatively described without co-signaling effects. Biotechnol Bioeng. 99:1261–1272. 2008. View Article : Google Scholar : PubMed/NCBI
10	Srisawat N, Manotham K and Eiam-Ong S, Katavetin P, Praditpornsilpa K and Eiam-Ong S: Erythropoietin and its non-erythropoietic derivative: do they ameliorate renal tubulointerstitial injury in ureteral obstruction? Int J Urol. 15:1011–1017. 2008. View Article : Google Scholar
11	Kakavas S, Demestiha T, Vasileiou P and Xanthos T: Erythropoetin as a novel agent with pleiotropic effects against acute lung injury. Eur J Clin Pharmacol. 67:1–9. 2011. View Article : Google Scholar : PubMed/NCBI
12	Yokomaku Y, Sugimoto T, Kume S, et al: Asialoerythropoietin prevents contrast-induced nephropathy. J Am Soc Nephrol. 19:321–328. 2008. View Article : Google Scholar
13	Haslett C, Shen AS, Feldsien DC, et al: ¹¹¹Indium-labeled neutrophil migration into the lungs of bleomycin-treated rabbits assessed noninvasively by external scintigraphy. Am Rev Respir Dis. 140:756–763. 1989. View Article : Google Scholar
14	Lamy ML, Fallat RJ, Koeniger EL, et al: Pathophysiology of adult respiratory distress syndrome. Acta Anaesthesiol Belg. 23(Suppl): 64–77. 1975.
15	Abraham E: Neutrophils and acute lung injury. Crit Care Med. 31:S195–S199. 2003. View Article : Google Scholar : PubMed/NCBI
16	Nakazawa J, Isshiki K, Sugimoto T, et al: Renoprotective effects of asialoerythropoietin in diabetic mice against ischaemia-reperfusion-induced acute kidney injury. Nephrology (Carlton). 15:93–101. 2010. View Article : Google Scholar : PubMed/NCBI
17	Imai Y and Osawa T: Elevation of the activities of glycosyl transferases involved in polylactosaminoglycan biosynthesis in autoimmune MRL lpr/lpr mouse T cells. Mol Immunol. 27:335–342. 1990. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Asialoerythropoietin ameliorates bleomycin‑induced acute lung injury in rabbits by reducing inflammation

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles