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Article

Association between the transcriptional levels of Htr‑1a and tryptophan hydroxylase‑1 in the hippocampus and the antifatigue effects of leucine on rats with postoperative fatigue

  • Authors:
    • Tiantian Wu
    • Jing Chen
    • Jiang Zhu
    • Zhen Yu
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Department of Rheumatology, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Department of General Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai 200072, P.R. China
  • Pages: 1633-1637
    |
    Published online on: September 17, 2014
       https://doi.org/10.3892/etm.2014.1973
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Abstract

Leucine (Leu), a branched‑chain amino acid (BCAA), is widely used in clinical practice following severe burns, gastrointestinal surgery, trauma and sepsis. In the present study, the antifatigue effects of BCAAs on a postoperative fatigue (POF) rat model, induced by 70% intestinal resection, were investigated. Leu (16.5 g/l) was administered intraperitoneally at a dose of 18 ml/kg/day. The fatigue level and antifatigue effects of Leu were evaluated by open‑field testing on day 1, 3, 5 and 7 after surgery. In addition, mRNA specimens were extracted and measured using a quantitative polymerase chain reaction method. The open‑field test results indicated that Leu exhibited a significant antifatigue effect. The total distance travelled and the number of times the rats passed from the outermost grids of an open‑top case were greatly improved in the Leu treatment group when compared with the POF model group. With the exception of the normal group, the mRNA expression levels of Htr‑1a exhibited a similar trend in all other groups, reaching a climax on day 3 and 5, while being restored to a normal level on day 7. With regard to the Leu intervention group, the mRNA expression level of Htr‑1a decreased significantly on day 3 and 5 following surgery. The mRNA expression levels of tryptophan hydroxylase‑1 were unchanged in this short time period; however, the levels were increased gradually in the Leu treatment group. Therefore, Leu exhibited an apparent antifatigue effect on various 5‑hydroxytryptamine‑associated genes.
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Copy and paste a formatted citation
Spandidos Publications style
Wu T, Chen J, Zhu J and Yu Z: Association between the transcriptional levels of Htr‑1a and tryptophan hydroxylase‑1 in the hippocampus and the antifatigue effects of leucine on rats with postoperative fatigue. Exp Ther Med 8: 1633-1637, 2014.
APA
Wu, T., Chen, J., Zhu, J., & Yu, Z. (2014). Association between the transcriptional levels of Htr‑1a and tryptophan hydroxylase‑1 in the hippocampus and the antifatigue effects of leucine on rats with postoperative fatigue. Experimental and Therapeutic Medicine, 8, 1633-1637. https://doi.org/10.3892/etm.2014.1973
MLA
Wu, T., Chen, J., Zhu, J., Yu, Z."Association between the transcriptional levels of Htr‑1a and tryptophan hydroxylase‑1 in the hippocampus and the antifatigue effects of leucine on rats with postoperative fatigue". Experimental and Therapeutic Medicine 8.5 (2014): 1633-1637.
Chicago
Wu, T., Chen, J., Zhu, J., Yu, Z."Association between the transcriptional levels of Htr‑1a and tryptophan hydroxylase‑1 in the hippocampus and the antifatigue effects of leucine on rats with postoperative fatigue". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1633-1637. https://doi.org/10.3892/etm.2014.1973
Copy and paste a formatted citation
x
Spandidos Publications style
Wu T, Chen J, Zhu J and Yu Z: Association between the transcriptional levels of Htr‑1a and tryptophan hydroxylase‑1 in the hippocampus and the antifatigue effects of leucine on rats with postoperative fatigue. Exp Ther Med 8: 1633-1637, 2014.
APA
Wu, T., Chen, J., Zhu, J., & Yu, Z. (2014). Association between the transcriptional levels of Htr‑1a and tryptophan hydroxylase‑1 in the hippocampus and the antifatigue effects of leucine on rats with postoperative fatigue. Experimental and Therapeutic Medicine, 8, 1633-1637. https://doi.org/10.3892/etm.2014.1973
MLA
Wu, T., Chen, J., Zhu, J., Yu, Z."Association between the transcriptional levels of Htr‑1a and tryptophan hydroxylase‑1 in the hippocampus and the antifatigue effects of leucine on rats with postoperative fatigue". Experimental and Therapeutic Medicine 8.5 (2014): 1633-1637.
Chicago
Wu, T., Chen, J., Zhu, J., Yu, Z."Association between the transcriptional levels of Htr‑1a and tryptophan hydroxylase‑1 in the hippocampus and the antifatigue effects of leucine on rats with postoperative fatigue". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1633-1637. https://doi.org/10.3892/etm.2014.1973
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