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Print ISSN: 1792-0981 Online ISSN: 1792-1015
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November-2014 Volume 8 Issue 5

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Article

Karyotype stability of human umbilical cord‑derived mesenchymal stem cells during in vitro culture

  • Authors:
    • Zhong‑Bao Ruan
    • Li Zhu
    • Yi‑Gang Yin
    • Ge‑Cai Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China
  • Pages: 1508-1512
    |
    Published online on: September 18, 2014
       https://doi.org/10.3892/etm.2014.1977
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Abstract

The aim of this study was to investigate whether the chromosomes of human umbilical cord‑derived mesenchymal stem cells (hUCMSCs) change following in vitro culture for several generations. In the present study, umbilical cords from two healthy infants following cesarean delivery were collected aseptically and hUCMSCs were isolated by digestion with collagenase and trypsin, and then cultured in vitro. hUCMSCs with fibroblastic morphology were presented from the human umbilical cord tissue after 7 days of adherent culture. When cultured for 6 passages in vitro, the hUCMSCs maintained a stable spindle‑shaped morphology. Cells reached the logarithmic growth phase after 3‑4 days of culture. In addition, CD13, CD29, CD44, CD90 and CD105 were highly expressed in generations P3‑P6. The expression of CD31, CD34, CD45 and HLA‑DR was negative. Furthermore, karyotype analysis revealed a normal diploid karyotype with 46 chromosomes and no abnormal changes were found in chromosome structure. These findings suggest that when cultured for 6 passages in vitro, hUCMSCs maintain a stable immunophenotype and chromosome structure, which provides an experimental basis for the safety of hUCMSC cytotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Ruan ZB, Zhu L, Yin YG and Chen GC: Karyotype stability of human umbilical cord‑derived mesenchymal stem cells during in vitro culture. Exp Ther Med 8: 1508-1512, 2014.
APA
Ruan, Z., Zhu, L., Yin, Y., & Chen, G. (2014). Karyotype stability of human umbilical cord‑derived mesenchymal stem cells during in vitro culture. Experimental and Therapeutic Medicine, 8, 1508-1512. https://doi.org/10.3892/etm.2014.1977
MLA
Ruan, Z., Zhu, L., Yin, Y., Chen, G."Karyotype stability of human umbilical cord‑derived mesenchymal stem cells during in vitro culture". Experimental and Therapeutic Medicine 8.5 (2014): 1508-1512.
Chicago
Ruan, Z., Zhu, L., Yin, Y., Chen, G."Karyotype stability of human umbilical cord‑derived mesenchymal stem cells during in vitro culture". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1508-1512. https://doi.org/10.3892/etm.2014.1977
Copy and paste a formatted citation
x
Spandidos Publications style
Ruan ZB, Zhu L, Yin YG and Chen GC: Karyotype stability of human umbilical cord‑derived mesenchymal stem cells during in vitro culture. Exp Ther Med 8: 1508-1512, 2014.
APA
Ruan, Z., Zhu, L., Yin, Y., & Chen, G. (2014). Karyotype stability of human umbilical cord‑derived mesenchymal stem cells during in vitro culture. Experimental and Therapeutic Medicine, 8, 1508-1512. https://doi.org/10.3892/etm.2014.1977
MLA
Ruan, Z., Zhu, L., Yin, Y., Chen, G."Karyotype stability of human umbilical cord‑derived mesenchymal stem cells during in vitro culture". Experimental and Therapeutic Medicine 8.5 (2014): 1508-1512.
Chicago
Ruan, Z., Zhu, L., Yin, Y., Chen, G."Karyotype stability of human umbilical cord‑derived mesenchymal stem cells during in vitro culture". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1508-1512. https://doi.org/10.3892/etm.2014.1977
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