Effect of Hyssopus officinalis L. on inhibiting airway inflammation and immune regulation in a chronic asthmatic mouse model

Ma,Xiaojuan; Ma,Xiumin; Ma,Zhixing; Wang,Jing; Sun,Zhan; Yu,Wenyan; Li,Fengsen; Ding,Jianbing

doi:10.3892/etm.2014.1978

Effect of Hyssopus officinalis L. on inhibiting airway inflammation and immune regulation in a chronic asthmatic mouse model

Authors:
- Xiaojuan Ma
- Xiumin Ma
- Zhixing Ma
- Jing Wang
- Zhan Sun
- Wenyan Yu
- Fengsen Li
- Jianbing Ding
View Affiliations

Affiliations: Xinjiang National Clinical Research Base of Traditional Chinese Medicine, Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China, Department of Clinical Laboratory, First Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830054, P.R. China, Ürümqi General Hospital of Lanzhou Military Area Command, Ürümqi, Xinjiang 830000, P.R. China, Department of Immunology, College of Basic Medicine, Ürümqi, Xinjiang 830011, P.R. China
Published online on: September 18, 2014 https://doi.org/10.3892/etm.2014.1978
Pages: 1371-1374

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The Uygur herb, Hyssopus officinalis L., has been demonstrated to affect the levels of a number of cytokines in asthmatic mice, including interleukin‑4, ‑6 and ‑17 and interferon‑γ. In the present study, the effect of Hyssopus officinalis L. on airway immune regulation and airway inflammation was investigated in a mouse model of chronic asthma. A total of 32 BALB/c mice were randomly divided into four groups, which included the normal, chronic asthmatic, dexamethasone treatment and Hyssopus officinalis L. treatment groups. Mice were sensitized and challenged with ovalbumin to establish an asthma model and the ratio of eosinophils (EOS) in the bronchoalveolar lavage fluid (BALF) was determined. In addition, the levels of immunoglobulin (Ig)E and IgG were detected using an enzyme‑linked immunosorbent assay. The degree of airway mucus secretion was observed using the periodic acid‑Schiff stain method. The results demonstrated that the ratio of EOS in the BALF and the level of serum IgE in the chronic asthmatic and dexamethasone treatment groups increased, while the level of serum IgG decreased, when compared with the normal group. In addition, excessive secretion of airway mucus was observed in these two groups. However, the EOS ratio in the BALF and the levels of serum IgE and IgG in the Hyssopus officinalis L. treatment group were similar to the results observed in the normal group. In conclusion, Hyssopus officinalis L. not only plays an anti‑inflammatory role by inhibiting the invasion of EOS and decreasing the levels of IgE, but also affects immune regulation.

View Figures

View References

1	Lemanske RF Jr and Busse WW: Asthma: clinical expression and molecular mechanisms. J Allergy Clin Immunol. 125(Suppl 2): S95–S102. 2010. View Article : Google Scholar : PubMed/NCBI
2	Herrick CA and Bottomly K: To respond or not to respond: T cells in allergic asthma. Nat Rev Immunol. 3:405–412. 2003. View Article : Google Scholar : PubMed/NCBI
3	Wei M, Chu X, Guan M, et al: Protocatechuicacid suppresses ovalbumin-induced airway inflammation in a mouse allergic asthma model. Int Immunopharmacol. 4:780–788. 2013. View Article : Google Scholar : PubMed/NCBI
4	Wang W, Huang KW, Wu BM, Wang YJ and Wang C: Correlation of eosinophil counts in induced sputum and fractional concentration of exhaled nitric oxide and lung functions in patients with mild to moderate asthma. Chin Med J (Engl). 125:3157–3160. 2012.PubMed/NCBI
5	Hannah JG and Sutton BJ: IgE in allergy and asthma today. Nat Rev Immunol. 8:205–217. 2008. View Article : Google Scholar
6	Rogers DF: Airway mucus hypersecretion in asthma: an undervalued pathology? Curr Opin Pharmacol. 4:241–250. 2004. View Article : Google Scholar : PubMed/NCBI
7	Zhao KZ, Lu JR, Zhang YF, et al: Changes of the levels of interleukin-13 and total immunoglobulin E in children with asthma. Jilin Da Xue Xue Bao Yi Xue Ban. 29:212–213. 2003.(In Chinese).
8	Wang YN, Ma J, Ma XM, et al: Effects of Uygur herb Hyssopus officinalis L. on cytokines in allergic asthma mice. Shanghai Zhong Yiyao Daxue Xuebao. 22:58–60. 2008.
9	Hou M, Zhu M, Ma XM, et al: Effects of Uygur medicine Hyssopus officinalis L. on serum IL-17 level and balance of Th1/Th2 of asthma rats. Zhongguo Xian Dai Yi Xue Za Zhi. 20:365–368. 2010.(In Chinese).
10	Hou M, Ma XM, Ding JB, et al: Effect of Uygur medicine Hyssopus officinalis L. on serum eotaxin-2, eotaxin-3 and sP-selectin level of asthma rats. Science & Technology Review. 28:90–93. 2009.
11	Ma XP, Ma XM, Ding JB, et al: Extraction process of polysaccharide from Hyssopus officinalis. Chin JMAP. 28:437–439. 2011.
12	Du Q, Zhang Q, Shen L, et al: Effects of astragaloside IV on airway remodeling in a murine model of chronic asthma. Zhongguo Yao Li Xue Tong Bao. 27:1430–1434. 2011.(In Chinese).
13	Kim MS, Cho KA, Cho YJ and Woo SY: Effects of interleukin-9 blockade on chronic airway inflammation in murine asthma models. Allergy Asthma Immunol Res. 5:197–206. 2013. View Article : Google Scholar : PubMed/NCBI
14	Chong L, Zhang W, Nie Y, Yu G, Liu L, et al: Protective effect of curcumin on acute airway inflammation of allergic asthma in mice through Notch1–GATA3 signaling pathway. Inflammation. Apr 6–2014.(Epub ahead of print).
15	Blonder JP, Mutka SC, Sun X, Qiu J, et al: Pharmacologic inhibition of S-nitrosoglutathione reductase protects against experimental asthma in BALB/c mice through attenuation of both bronchoconstriction and inflammation. BMC Pulm Med. 14:32014. View Article : Google Scholar
16	Asano T, Kume H, Taki F, et al: Thalidomide attenuates airway hyperresponsiveness and eosinophilic inflammation in a murine model of allergic asthma. Biol Pharm Bull. 33:1028–1032. 2010. View Article : Google Scholar : PubMed/NCBI
17	Lu S, Li C, Ming M and Luo Z: Inhalation of inactivated Mycobacterium phlei down-regulates expression of nuclear factor-kappa B and intercellular adhesion molecule in asthmatic mice. Chinese J Pathophysiol. 30:333–338. 2014.
18	Zhang W, Zhu T, Wang D, et al: Rosuvastatin attenuates airway inflammation of chronic asthma in mice. Med J West China. 26:150–153. 2014.
19	Chu Y: Progress in the association of high affinity IgE receptor with asthma. Int J Pediatrics. 38:280–282. 2011.
20	Stone KD, Prussin C and Metcalfe DD: IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol. 125(Suppl 2): S73–S80. 2010. View Article : Google Scholar : PubMed/NCBI
21	Locksley RM: Asthma and allergic inflammation. Cell. 140:777–783. 2010. View Article : Google Scholar : PubMed/NCBI
22	Hargreave FE and Nair P: Point: Is measuring sputum eosinophils useful in the management of severe asthma? Yes Chest. 139:1270–1273. 2011. View Article : Google Scholar : PubMed/NCBI
23	Regina MCPinto, Alberto C, Luciene A, et al: Clinical characteristics and possible phenotypes of an adult severe asthma population. Respir Med. 106:47–56. 2012. View Article : Google Scholar : PubMed/NCBI
24	Jatakanon A, Lim S, Kharitonov SA, Chung KF and Barnes PJ: Correlation between exhaled nitric oxide, sputum eosinophils, and methacholine responsiveness in patients with mild asthma. Thorax. 53:91–95. 1998. View Article : Google Scholar : PubMed/NCBI
25	Ichiki H, Hoshino T, Kinoshita T, et al: Thioredoxin suppresses airway hyperresponsiveness and airway inflammation in asthma. Biochem Biophys Res Commun. 334:1141–1148. 2005. View Article : Google Scholar : PubMed/NCBI
26	Liu YM and Wushati Y: Hyssopus offcinalis L.. J Uygur Med. Xinjiang Science & Technology & Hygiene Publishing House; Ürümqi, China: pp. 423–429. 1999