Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2014 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2014 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Detection of Her‑2/neu expression in gastric cancer: Quantitative PCR versus immunohistochemistry

  • Authors:
    • Guang‑Jun Zhu
    • Chun‑Wei Xu
    • Mei‑Yu Fang
    • Yu‑Ping Zhang
    • Yang Li
  • View Affiliations / Copyright

    Affiliations: Department of Central Laboratory, The 281st Hospital of the PLA, Qinhuangdao, Hebei 066105, P.R. China, Department of Pathology, The General Military Hospital of Beijing PLA, Beijing 100700, P.R. China, Department of Integrated Chinese Traditional Medicine and Western Medicine, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310021, P.R. China, Department of Pathology, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China, Department of Oncology, The General Military Hospital of Beijing PLA, Beijing 100700, P.R. China
  • Pages: 1501-1507
    |
    Published online on: September 22, 2014
       https://doi.org/10.3892/etm.2014.1982
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to compare quantitative polymerase chain reaction (qPCR) with immunohistochemistry (IHC) for the detection of Her‑2 in gastric cancer, and to investigate the correlation between the expression levels of human epidermal growth factor receptor 2 (Her‑2) and clinical features. Clinical data from 426 cases of gastric cancer were collected. Her‑2 expression levels in cancerous tissue were detected using IHC, and the Her‑2/neu gene expression levels were determined by qPCR. The correlation between the expression level of Her‑2 and clinical features was investigated. The positive expression rate of Her‑2 in cancerous tissue detected using qPCR and IHC was 11.17% (46/412) and 13.38% (57/426), respectively. The positive expression of the Her‑2 protein/gene was significantly correlated with the depth of invasion and lymphatic metastasis, as well as the TNM stage (P<0.05). No significant correlation was identified between positive expression of the Her‑2 protein/gene and tumor location, age, gender, differentiation degree and Lauren classification (P>0.05). The diagnostic consistency was good between the two methods (κ=0.828). The results indicate that the expression of Her‑2/neu is closely associated with the development of gastric cancer. qPCR is a convenient, objective and efficient method, which may be used as an alternative to IHC or fluorescence in situ hybridization for the detection of Her‑2/neu gene.
View Figures

Figure 1

Figure 2

View References

1 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar

2 

Jemal A, Bray F, Center MM, et al: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

3 

Ferlay J, Shin HR, Bray F, et al: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Zou XN, Duan JJ, Huangfu XM, Chen WQ and Zhao P: Analysis of stomach cancer mortality in the national retrospective sampling survey of death causes in China. Chin J Prev Med. 44:390–397. 2010.(In Chinese).

5 

Arteaga C: Targeting Her1/EGFR: a molecular approach to cancer therapy. Semin Oncol. 30(Suppl 7): 3–14. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Park DI, Yun JW, Park JH, et al: Her-2/neu amplification is an independent prognostic factor in gastric cancer. Dig Dis Sci. 51:1371–1379. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Hamilton SR and Aaltonen LA: World Health Organization classification of tumors. Pathology and Genetics of Tumours of the Digestive System. IARC Press; Lyon, France: pp. 265–314. 2000

8 

Ichikura T, Tomimatsu S, Uefuji K, et al: Evaluation of the New American Joint Committee on Cancer/International Union against cancer classification of lymph node metastasis from gastric carcinoma in comparison with the Japanese classification. Cancer. 86:553–558. 1999. View Article : Google Scholar

9 

Moelans CB, van Diest PJ, Milne AN and Offerhaus GJ: Her-2/neu testing and therapy in gastroesophageal adenocarcinoma. Patholog Res Int. 2011:6741822010.PubMed/NCBI

10 

Casalini P, Iorio MV, Galmozzi E and Ménard S: Role of HER receptors family in development and differentiation. J Cell Physiol. 200:343–350. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Gravalos C and Jimeno A: HER2 in gastric cancer: a new prognostic factor and a novel therapeutic target. Ann Oncol. 19:1523–1529. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Wolff AC, Hammond ME, Schwartz JN, et al: American Society of Clinical Oncology/College of American Pathologists: American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. Arch Pathol Lab Med. 131:18–43. 2007.

13 

Bang YJ, Van Cutsem E, Feyereislova A, et al: Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer (ToGA): a phase 3, open-label, randomised controlled trial. Lancet. 376:687–697. 2010. View Article : Google Scholar

14 

Hayes DF, Yamauchi H, Broadwater G, et al: Cancer and Leukemia Group B: Circulating Her-2/erbB-2/c-neu (Her-2) extracellular domain as a prognostic factor in patients with metastatic breast cancer: Cancer and Leukemia Group B Study 8662. Clin Cancer Res. 7:2703–2711. 2001.PubMed/NCBI

15 

Tsugawa K, Yonemura Y, Hirono Y, et al: Amplification of the c-met, c-erbB-2 and epidermal growth factor receptor gene in human gastric cancers: correlation to clinical features. Oncology. 55:475–481. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Yonemura Y, Ninomiya I, Tsugawa K, et al: Prognostic significance of c-erbB-2 gene expression in the poorly differentiated type of adenocarcinoma of the stomach. Cancer Detect Prev. 22:139–146. 1998. View Article : Google Scholar : PubMed/NCBI

17 

Yonemura Y, Ninomiya I, Yamaguchi A, et al: Evaluation of immunoreactivity for erbB-2 protein as a marker of poor short term prognosis in gastric cancer. Cancer Res. 51:1034–1038. 1991.PubMed/NCBI

18 

Uchino S, Tsuda H, Maruyama K, et al: Overexpression of c-erbB-2 protein in gastric cancer. Its correlation with long-term survival of patients. Cancer. 72:3179–3184. 1993. View Article : Google Scholar : PubMed/NCBI

19 

Barberis M, Pellegrini C, Cannone M, et al: Quantitative PCR and Her2 testing in breast cancer: a technical and cost-effectiveness analysis. Am J Clin Pathol. 129:563–570. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Bossard C, Bieche I, Le Doussal V, Lidereau R and Sabourin JC: Real-time RT-PCR: a complementary method to detect Her-2 status in breast carcinoma. Anticancer Res. 25:4679–4683. 2005.PubMed/NCBI

21 

Tse C, Brault D, Gligorov J, et al: Evaluation of the quantitative analytical methods real-time PCR for Her-2 gene quantification and ELISA of serum Her-2 protein and comparison with fluorescence in situ hybridization and immunohistochemistry for determining Her-2 status in breast cancer patients. Clin Chem. 51:1093–1101. 2005.

22 

Nistor A, Watson PH, Pettigrew N, et al: Real-time PCR complements immunohistochemistry in the determination of Her-2/neu status in breast cancer. BMC Clin Pathol. 6:22006. View Article : Google Scholar : PubMed/NCBI

23 

Chariyalertsak S, Purisa W and Vinyuvat S: Her-2/neu amplification determined by real-time quantitative PCR and its association with clinical outcome of breast cancer in Thailand. Asian Pac J Cancer Prev. 12:1703–1706. 2011.PubMed/NCBI

24 

Page K, Hava N, Ward B, et al: Detection of Her2 amplification in circulating free DNA in patients with breast cancer. Br J Cancer. 104:1342–1348. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhu GJ, Xu CW, Fang MY, Zhang YP and Li Y: Detection of Her‑2/neu expression in gastric cancer: Quantitative PCR versus immunohistochemistry. Exp Ther Med 8: 1501-1507, 2014.
APA
Zhu, G., Xu, C., Fang, M., Zhang, Y., & Li, Y. (2014). Detection of Her‑2/neu expression in gastric cancer: Quantitative PCR versus immunohistochemistry. Experimental and Therapeutic Medicine, 8, 1501-1507. https://doi.org/10.3892/etm.2014.1982
MLA
Zhu, G., Xu, C., Fang, M., Zhang, Y., Li, Y."Detection of Her‑2/neu expression in gastric cancer: Quantitative PCR versus immunohistochemistry". Experimental and Therapeutic Medicine 8.5 (2014): 1501-1507.
Chicago
Zhu, G., Xu, C., Fang, M., Zhang, Y., Li, Y."Detection of Her‑2/neu expression in gastric cancer: Quantitative PCR versus immunohistochemistry". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1501-1507. https://doi.org/10.3892/etm.2014.1982
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu GJ, Xu CW, Fang MY, Zhang YP and Li Y: Detection of Her‑2/neu expression in gastric cancer: Quantitative PCR versus immunohistochemistry. Exp Ther Med 8: 1501-1507, 2014.
APA
Zhu, G., Xu, C., Fang, M., Zhang, Y., & Li, Y. (2014). Detection of Her‑2/neu expression in gastric cancer: Quantitative PCR versus immunohistochemistry. Experimental and Therapeutic Medicine, 8, 1501-1507. https://doi.org/10.3892/etm.2014.1982
MLA
Zhu, G., Xu, C., Fang, M., Zhang, Y., Li, Y."Detection of Her‑2/neu expression in gastric cancer: Quantitative PCR versus immunohistochemistry". Experimental and Therapeutic Medicine 8.5 (2014): 1501-1507.
Chicago
Zhu, G., Xu, C., Fang, M., Zhang, Y., Li, Y."Detection of Her‑2/neu expression in gastric cancer: Quantitative PCR versus immunohistochemistry". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1501-1507. https://doi.org/10.3892/etm.2014.1982
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team