Levels of acylation stimulating protein and the complement component 3 precursor are associated with the occurrence and development of coronary heart disease

Jiang,Honglei; Guo,Miao; Dong,Linping; Cao,Chunlin; Wang,Dong; Liang,Xiaotang; Guo,Fang; Xing,Zhaoqin; Bu,Peili; Liu,Jidong

doi:10.3892/etm.2014.2018

December-2014 Volume 8 Issue 6

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December-2014 Volume 8 Issue 6

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Article

Levels of acylation stimulating protein and the complement component 3 precursor are associated with the occurrence and development of coronary heart disease

Authors:
- Honglei Jiang
- Miao Guo
- Linping Dong
- Chunlin Cao
- Dong Wang
- Xiaotang Liang
- Fang Guo
- Zhaoqin Xing
- Peili Bu
- Jidong Liu
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Affiliations: Department of Cardiology, Provincial Hospital of Shandong University, Jinan, Shandong 250021, P.R. China, Department of Gerontology, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China, Department of Orthopedics, Provincial Hospital of Shandong University, Jinan, Shandong 250021, P.R. China, Key Laboratory of Cardiovascular Remodeling and Function Research of Chinese Ministry of Education and Chinese Ministry of Health, Department of Cardiology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
Pages: 1861-1866
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Published online on: October 13, 2014

https://doi.org/10.3892/etm.2014.2018
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Abstract

The aim of the present study was to investigate whether acylation stimulating protein (ASP) and complement component 3 (C3) are associated with the occurrence and development of coronary heart disease (CHD). The participants of the study were divided into three groups, including the healthy control (n=42), metabolic syndrome (MS, n=56) and CHD (n=62) groups. An enzyme‑linked immunosorbent assay was used to measure the ASP concentrations, while an immunoturbidimetric assay was employed to determine the C3 concentrations. In addition, coronary angiography was performed to determine the severity of coronary artery disease in the CHD group. The CHD group was divided into three subgroups, according to the final Gensini score of coronary artery stenosis for each patient (mild, ≤20 points; moderate, 21‑40 points; severe, >40 points). Western blotting and quantitative reverse transcription‑polymerase chain reaction (RT‑PCR) were performed to analyze the protein and mRNA expression levels of C3 in the CHD subgroups and the healthy control group. The concentrations of ASP and C3 in the CHD group were found to be significantly higher compared with the control and MS groups. In addition, the levels of ASP and C3 in the mild and moderate CHD subgroups were significantly higher compared with the healthy controls or mild CHD patients. Furthermore, the protein expression levels of C3 in the moderate and severe CHD patients were found to be significantly higher compared with the healthy individuals and the mild CHD patients. The quantitative RT‑PCR results revealed that the mRNA expression levels of C3 in the moderate and severe CHD patients were significantly higher compared with the healthy control group and the mild CHD patients. Furthermore, the mean levels of C3 transcripts in the severe CHD patients were found to be higher compared with the moderate CHD subgroup (P<0.05). Therefore, ASP and C3 were found to be associated with the occurrence and development of CHD; thus, may be used as novel indexes for CHD.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Levels of acylation stimulating protein and the complement component 3 precursor are associated with the occurrence and development of coronary heart disease

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