Protease‑activated receptor 2 agonist increases cell proliferation and invasion of human pancreatic cancer cells

Xie,Liqun; Duan,Zexing; Liu,Caiju; Zheng,Yanmin; Zhou,Jing

doi:10.3892/etm.2014.2052

January-2015 Volume 9 Issue 1

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January-2015 Volume 9 Issue 1

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Article

Protease‑activated receptor 2 agonist increases cell proliferation and invasion of human pancreatic cancer cells

Authors:
- Liqun Xie
- Zexing Duan
- Caiju Liu
- Yanmin Zheng
- Jing Zhou
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, Affiliated Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, P.R. China
Pages: 239-244
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Published online on: November 5, 2014

https://doi.org/10.3892/etm.2014.2052
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Abstract

The aim of this study was to determine the expression of protease‑activated receptor 2 (PAR‑2) in the human pancreatic cancer cell line SW1990, and to evaluate its effect on cell proliferation and invasion. The expression of PAR‑2 protein and mRNA in SW1990 cells was determined by immunocytochemistry and reverse transcription polymerase chain reaction (PCR), respectively. MTT and cell invasion and migration assays, as well as semi‑quantitative PCR and zymography analysis, were additionally performed. PAR‑2 mRNA was significantly upregulated in the cells treated with trypsin or the PAR‑2 activating peptide Ser‑Leu‑Ile‑Gly‑Lys‑Val (SLIGKV) (P<0.01), but not in the Val‑Lys‑Gly‑Ile‑Leu‑Ser group (P>0.05). Trypsin and SLIGKV significantly promoted SW1990 cell proliferation in a dose‑ and time‑dependent manner (P<0.05). Compared with the control group, trypsin and SLIGKV significantly increased the mRNA expression (P<0.01) and gelatinolytic activity (P<0.01) of matrix metalloproteinase (MMP)‑2. In conclusion, PAR‑2 is expressed in SW1990 cells. PAR‑2 activation may promote the invasion and migration of human pancreatic cancer cells by increasing MMP‑2 expression.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Protease‑activated receptor 2 agonist increases cell proliferation and invasion of human pancreatic cancer cells

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