Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
January-2015 Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults

  • Authors:
    • Yuxian Chen
    • Chun Zeng
    • Hua Zeng
    • Rongkai Zhang
    • Zhiqiang Ye
    • Bangrong Xing
    • Kunhua Hu
    • Mingtao Li
    • Dao Zhang Cai
  • View Affiliations / Copyright

    Affiliations: Department of Joint Surgery and Orthopaedic Trauma, The Third Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China, Department of Joint Surgery, The Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong 510630, P.R. China, Department of Joint Surgery and Orthopaedic Trauma, The Fifth Affiliated Hospital, Sun Yat‑Sen University, Zhuhai, Guangdong 510630, P.R. China, Proteomics Laboratory, Zhongshan Medical College, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China
  • Pages: 77-83
    |
    Published online on: November 12, 2014
       https://doi.org/10.3892/etm.2014.2069
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Steroid‑induced osteonecrosis of the femoral head (SONFH) is a disabling, aseptic and ischemic disease that develops following steroid therapy. The pathogenesis of SONFH is unclear, so the early diagnosis and treatment for this disease is yet to be established. The purpose of the present study was to identify potential biomarkers for SONFH. The differential expression of serum proteins from patients with SONFH and healthy volunteers was analyzed by the proteomics method. The protein samples were labeled and subjected to isoelectric focusing and two‑dimensional gel electrophoresis. The resultant protein spots were matched and quantified by an imaging analysis system. The differentially‑expressed protein spots were subjected to in‑gel trypsin digestion followed by matrix‑assisted laser desorption ionization time‑of‑flight mass spectrometry. Significantly lower levels of complement component 3 (C3), C4, inter‑α‑trypsin inhibitor heavy chain H4 and α‑2‑macroglobulin were found in the serum of patients with SONFH. These proteins are reported to be actively involved in intravascular coagulation, apoptosis and reactive oxygen species imbalance, indicating that multiple pathological reactions occur in SONFH and these proteins may serve as potential biomarkers for the diagnosis of SONFH.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Kubo T, Fujioka M and Ishida M: Clinical condition of steroid-induced osteonecrosis of the femoral head. Clin Calcium. 17:856–862. 2007.(In Japanese). PubMed/NCBI

2 

Kerachian MA, Séguin C and Harvey EJ: Glucocorticoids in osteonecrosis of the femoral head: a new understanding of the mechanisms of action. J Steroid Biochem Mol Biol. 114:121–128. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Thiede B and Rudel T: Proteome analysis of apoptotic cells. Mass Spectrom Rev. 23:333–349. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Liu W, Zhou XW, Liu S, et al: Calpain-truncated CRMP-3 and -4 contribute to potassium deprivation-induced apoptosis of cerebellar granule neurons. Proteomics. 9:3712–3728. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Liao H, Wu J, Kuhn E, et al: Use of mass spectrometry to identify protein biomarkers of disease severity in the synovial fluid and serum of patients with rheumatoid arthritis. Arthritis Rheum. 50:3792–3803. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Liu J, Zhu P, Peng J, et al: Identification of disease-associated proteins by proteomic approach in ankylosing spondylitis. Biochem Biophys Res Commun. 357:531–536. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Hermansson M, Sawaji Y, Bolton M, et al: Proteomic analysis of articular cartilage shows increased type II collagen synthesis in osteoarthritis and expression of inhibin betaA (activin A), a regulatory molecule for chondrocytes. J Biol Chem. 279:43514–43521. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Pastorelli R, Carpi D, Airoldi L, et al: Proteome analysis for the identification of in vivo estrogen-regulated proteins in bone. Proteomics. 5:4936–4945. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Jawad MU, Haleem AA and Scully SP: In brief: Ficat classification: avascular necrosis of the femoral head. Clin Orthop Relat Res. 470:2636–2639. 2012. View Article : Google Scholar : PubMed/NCBI

10 

National Center for Biotechnology Information. http://www.ncbi.nlm.nih.gov/uri. Accessed August 15, 2013

11 

Expasy Bioinformatics Resource Portal. UniProtKB/Swiss-Prot guideline. http://web.expasy.org/docs/swiss-prot_guideline.htmluri. Accessed August 20, 2013

12 

Yu KH, Rustgi AK and Blair IA: Characterization of proteins in human pancreatic cancer serum using differential gel electrophoresis and tandem mass spectrometry. J Proteome Res. 4:1742–1751. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Baldo A, Sniderman AD, St-Luce S, et al: The adipsin-acylation stimulating protein system and regulation of intracellular triglyceride synthesis. J Clin Invest. 92:1543–1547. 1993. View Article : Google Scholar : PubMed/NCBI

14 

Wu RW, Wang FS, Ko JY, Wang CJ and Wu SL: Comparative serum proteome expression of osteonecrosis of the femoral head in adults. Bone. 43:561–566. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Luukkainen R, Hakala M, Sajanti E, Huhtala H, Yli-Kerttula U and Hameenkorpi R: Predictive value of synovial fluid analysis in estimating the efficacy of intra-articular corticosteroid injections in patients with rheumatoid arthritis. Ann Rheum Dis. 51:874–876. 1992. View Article : Google Scholar : PubMed/NCBI

16 

Familian A, Voskuyl AE, van Mierlo GJ, et al: Infliximab treatment reduces complement activation in patients with rheumatoid arthritis. Ann Rheum Dis. 64:1003–1008. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Lampreave F, Gonzalez-Ramon N, Martinez-Ayensa S, et al: Characterization of the acute phase serum protein response in pigs. Electrophoresis. 15:672–676. 1994. View Article : Google Scholar : PubMed/NCBI

18 

Pineiro M, Andres M, Iturralde M, et al: ITIH4 (inter-alpha-trypsin inhibitor heavy chain 4) is a new acute-phase protein isolated from cattle during experimental infection. Infect Immun. 72:3777–3782. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Daveau M, Jean L, Soury E, et al: Hepatic and extra-hepatic transcription of inter-alpha-inhibitor family genes under normal or acute inflammatory conditions in rat. Arch Biochem Biophys. 350:315–323. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Pineiro M, Alava MA, Gonzalez-Ramon N, et al: ITIH4 serum concentration increases during acute-phase processes in human patients and is up-regulated by interleukin-6 in hepatocarcinoma HepG2 cells. Biochem Biophys Res Commun. 263:224–229. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Bost F, Diarra-Mehrpour M and Martin JP: Inter-alpha-trypsin inhibitor proteoglycan family-a group of proteins binding and stabilizing the extracellular matrix. Eur J Biochem. 252:339–346. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Tu G, Xu W, Huang H and Li S: Progress in the development of matrix metalloproteinase inhibitors. Curr Med Chem. 15:1388–1395. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Borth W: Alpha 2-macroglobulin, a multifunctional binding protein with targeting characteristics. FASEB J. 6:3345–3353. 1992.PubMed/NCBI

24 

Baker AH, Edwards DR and Murphy G: Metalloproteinase inhibitors: biological actions and therapeutic opportunities. J Cell Sci. 115:3719–3727. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Zorin NA, Zorina VN and Zorina RM: Role of alpha-2 macroglobulin in oncologic diseases. Vopr Onkol. 50:515–519. 2004.(In Russian).

26 

Mocchegiani E, Costarelli L, Giacconi R, Cipriano C, Muti E and Malavolta M: Zinc-binding proteins (metallothionein and alpha-2 macroglobulin) and immunosenescence. Exp Gerontol. 41:1094–1107. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Zhang Y, Ge G and Greenspan DS: Inhibition of bone morphogenetic protein 1 by native and altered forms of alpha2-macroglobulin. J Biol Chem. 281:39096–39104. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Simpson ML, Goldenberg NA, Jacobson LJ, Bombardier CG, Hathaway WE and Manco-Johnson MJ: Simultaneous thrombin and plasmin generation capacities in normal and abnormal states of coagulation and fibrinolysis in children and adults. Thromb Res. 127:317–323. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Kerachian MA, Cournoyer D, Harvey EJ, et al: New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: microarray analysis of gene expression in a rat model. Arthritis Res Ther. 12:R1242010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Zeng C, Zeng H, Zhang R, Ye Z, Xing B, Hu K, Li M and Cai DZ: Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults. Exp Ther Med 9: 77-83, 2015.
APA
Chen, Y., Zeng, C., Zeng, H., Zhang, R., Ye, Z., Xing, B. ... Cai, D.Z. (2015). Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults. Experimental and Therapeutic Medicine, 9, 77-83. https://doi.org/10.3892/etm.2014.2069
MLA
Chen, Y., Zeng, C., Zeng, H., Zhang, R., Ye, Z., Xing, B., Hu, K., Li, M., Cai, D. Z."Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults". Experimental and Therapeutic Medicine 9.1 (2015): 77-83.
Chicago
Chen, Y., Zeng, C., Zeng, H., Zhang, R., Ye, Z., Xing, B., Hu, K., Li, M., Cai, D. Z."Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults". Experimental and Therapeutic Medicine 9, no. 1 (2015): 77-83. https://doi.org/10.3892/etm.2014.2069
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Zeng C, Zeng H, Zhang R, Ye Z, Xing B, Hu K, Li M and Cai DZ: Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults. Exp Ther Med 9: 77-83, 2015.
APA
Chen, Y., Zeng, C., Zeng, H., Zhang, R., Ye, Z., Xing, B. ... Cai, D.Z. (2015). Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults. Experimental and Therapeutic Medicine, 9, 77-83. https://doi.org/10.3892/etm.2014.2069
MLA
Chen, Y., Zeng, C., Zeng, H., Zhang, R., Ye, Z., Xing, B., Hu, K., Li, M., Cai, D. Z."Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults". Experimental and Therapeutic Medicine 9.1 (2015): 77-83.
Chicago
Chen, Y., Zeng, C., Zeng, H., Zhang, R., Ye, Z., Xing, B., Hu, K., Li, M., Cai, D. Z."Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults". Experimental and Therapeutic Medicine 9, no. 1 (2015): 77-83. https://doi.org/10.3892/etm.2014.2069
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team