Rapid identification of apolipoprotein E genotypes by high-resolution melting analysis in Chinese Han and African Fang populations

Zhan,Xiu‑Hui; Zha,Guang‑Cai; Jiao,Ji‑Wei; Yang,Li‑Ye; Zhan,Xiao‑Fen; Chen,Jiang‑Tao; Xie,Dong‑De; Eyi,Urbano  Monsuy; Matesa,Rocio  Apicante; Obono,Maximo   Miko Ondo; Ehapo,Carlos  Sala; Wei,Er‑Jia; Zheng,Yu‑Zhong; Yang,Hui; Lin,Min

doi:10.3892/etm.2014.2097

Rapid identification of apolipoprotein E genotypes by high-resolution melting analysis in Chinese Han and African Fang populations

Authors:
- Xiu‑Hui Zhan
- Guang‑Cai Zha
- Ji‑Wei Jiao
- Li‑Ye Yang
- Xiao‑Fen Zhan
- Jiang‑Tao Chen
- Dong‑De Xie
- Urbano Monsuy Eyi
- Rocio Apicante Matesa
- Maximo Miko Ondo Obono
- Carlos Sala Ehapo
- Er‑Jia Wei
- Yu‑Zhong Zheng
- Hui Yang
- Min Lin
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Affiliations: Department of Biology, Hanshan Normal University, Chaozhou, Guangdong 521000, P.R. China, Laboratory Medical Center, Chaozhou Central Hospital Affiliated to Southern Medical University, Chaozhou, Guangdong 521000, P.R. China, The Chinese Medical Aid Team to Equatorial Guinea, Guangzhou, Guangdong 510000, P.R. China, Central Blood Transfusion Service, Malabo Regional Hospital, Malabo 999115, Equatorial Guinea
Published online on: December 1, 2014 https://doi.org/10.3892/etm.2014.2097
Pages: 469-475

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Abstract

Apolipoprotein E (APOE) gene polymorphism can affect APOE gene transcription, serum lipid levels and repair of tissue damage, which could place individuals at serious risk of cardiovascular disease or certain infectious diseases. Recently, high‑resolution melting (HRM) analysis was reported to be a simple, inexpensive, accurate and sensitive method for the genotyping or/and scanning of rare mutations. For this reason, an HRM analysis was used in the present study for APOE genotyping in the Southern Chinese Han and African Fang populations. A total of 100 healthy Southern Chinese Han and 175 healthy African Fang individuals attended the study. Polymerase chain reaction‑DNA sequencing was used as a reference method for the genotyping of these samples. The six APOE genotypes could all be rapidly and efficiently identified by HRM analysis, and 100% concordance was found between the HRM analysis and the reference method. The allele frequencies of APOE in the Southern Chinese Han population were 7.0, 87.5 and 5.5% for ε2, ε3 and ε4, respectively. In the African Fang population, the allele frequencies of APOE were 24.3, 65.7 and 10.0% for ε2, ε3 and ε4, respectively. A statistically significant difference was found between the allele frequencies between the populations (P<0.05). In conclusion, the present study revealed the molecular characterization of APOE gene polymorphism in the Han population from the Chaozhou region of Southern China and the Fang population from Equatorial Guinea. The findings of the study indicated that HRM analysis could be used as an accurate and sensitive method for the rapid screening and identification of APOE genotypes in prospective clinical and population genetic analyses.

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