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Article

Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone

  • Authors:
    • Zhong‑Kui Xiong
    • Juan Lang
    • Gang Xu
    • Hai‑Yu Li
    • Yun Zhang
    • Lei Wang
    • Yao Su
    • Ai‑Jing Sun
  • View Affiliations / Copyright

    Affiliations: Department of Radiotherapy, Shaoxing Second Hospital, Shaoxing, Zhejiang 312000, P.R. China, Medical Research Center, Shaoxing People's Hospital, Zhejiang University, Shaoxing, Zhejiang 312000, P.R. China, Department of Radiotherapy, Jiangsu University Affiliated People's Hospital, Zhenjiang, Jiangsu 212002, P.R. China, Department of Laboratory Medicine, Shaoxing University School of Medicine, Shaoxing, Zhejiang 312099, P.R. China, Department of Basic Medicine, Shaoxing University School of Medicine, Shaoxing, Zhejiang 312099, P.R. China, Department of Clinical Medicine, Shaoxing University School of Medicine, Shaoxing, Zhejiang 312099, P.R. China, Department of Pathology, Shaoxing People's Hospital, Zhejiang University, Shaoxing, Zhejiang, Shaoxing, Zhejiang 312000, P.R. China
  • Pages: 553-558
    |
    Published online on: December 2, 2014
       https://doi.org/10.3892/etm.2014.2099
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Abstract

Systemic rotenone models of Parkinson's disease (PD) are highly reproducible and may provide evidence on the pathogenesis of PD. In the present study, male Sprague‑Dawley rats (1‑year‑old) were subcutaneously administered with rotenone (1.5 mg/kg/day) for six days and observed for the following three weeks. Compared with the control rats, a significant decrease was observed in the body weight and a marked increase was observed in the areas under the behavioral scoring curves in the rotenone‑treated rats. Immunohistochemical staining revealed that the abundance of nigral tyrosine hydroxylase (TH)‑positive neurons was markedly reduced following rotenone treatment. ELISA and neurochemical assays demonstrated a significant increase in the levels of nitric oxide (NO) and NO synthase, whereas a marked decrease was observed in the thiol levels in the brains of the rotenone‑treated rats. Thus, subacute rotenone treatment was found to induce behavioral deficits and the loss of nigral TH‑positive neurons which may be associated with the excessive levels of NO in the rat brains.
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Copy and paste a formatted citation
Spandidos Publications style
Xiong ZK, Lang J, Xu G, Li HY, Zhang Y, Wang L, Su Y and Sun AJ: Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone. Exp Ther Med 9: 553-558, 2015.
APA
Xiong, Z., Lang, J., Xu, G., Li, H., Zhang, Y., Wang, L. ... Sun, A. (2015). Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone. Experimental and Therapeutic Medicine, 9, 553-558. https://doi.org/10.3892/etm.2014.2099
MLA
Xiong, Z., Lang, J., Xu, G., Li, H., Zhang, Y., Wang, L., Su, Y., Sun, A."Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone". Experimental and Therapeutic Medicine 9.2 (2015): 553-558.
Chicago
Xiong, Z., Lang, J., Xu, G., Li, H., Zhang, Y., Wang, L., Su, Y., Sun, A."Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone". Experimental and Therapeutic Medicine 9, no. 2 (2015): 553-558. https://doi.org/10.3892/etm.2014.2099
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong ZK, Lang J, Xu G, Li HY, Zhang Y, Wang L, Su Y and Sun AJ: Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone. Exp Ther Med 9: 553-558, 2015.
APA
Xiong, Z., Lang, J., Xu, G., Li, H., Zhang, Y., Wang, L. ... Sun, A. (2015). Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone. Experimental and Therapeutic Medicine, 9, 553-558. https://doi.org/10.3892/etm.2014.2099
MLA
Xiong, Z., Lang, J., Xu, G., Li, H., Zhang, Y., Wang, L., Su, Y., Sun, A."Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone". Experimental and Therapeutic Medicine 9.2 (2015): 553-558.
Chicago
Xiong, Z., Lang, J., Xu, G., Li, H., Zhang, Y., Wang, L., Su, Y., Sun, A."Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone". Experimental and Therapeutic Medicine 9, no. 2 (2015): 553-558. https://doi.org/10.3892/etm.2014.2099
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