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Treatment with retinoic acid and lens epithelial cell-conditioned medium in vitro directed the differentiation of pluripotent stem cells towards corneal endothelial cell-like cells

  • Authors:
    • Ping Chen
    • Jun‑Zhao Chen
    • Chun‑Yi Shao
    • Chuan‑Yin Li
    • Yi‑Dan Zhang
    • Wen‑Juan Lu
    • Yao Fu
    • Ping Gu
    • Xianqun Fan
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 351-360
    |
    Published online on: December 3, 2014
       https://doi.org/10.3892/etm.2014.2103
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Abstract

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have extensive self‑renewal capacity and the potential to differentiate into all tissue‑specific cell lineages, including corneal endothelial cells (CECs). They are a promising prospect for the future of regenerative medicine. The method of derivation of CECs from ESCs and iPSCs, however, remains to be elucidated. In this study, mouse ESCs and iPSCs were induced to differentiate into CECs using CEC embryonic development events as a guide. All‑trans retinoic acid (RA) treatment during the embryoid body (EB) differentiation step was used to promote neural crest (NC) cell differentiation as first step and was followed by a second induction in CEC‑ or lens epithelial cell (LEC)‑conditioned medium (CM) to ultimately generate CEC‑like cells. During the corresponding differentiation stages, NC developmental markers and CEC differentiation markers were detected at the protein level using immunocytochemistry (ICC) and at the mRNA level by reverse transcription‑quantitative polymerase chain reaction (RT-qPCR). During the first stage, the data indicated that 4 days of treatment with 1 µM RA starting on day 4 of EB formation favored NC cell differentiation and that plating on gelatin‑coated plates led to cell migration out of the EBs. The second‑stage differentiation results showed that the CM, particularly the LEC‑CM, enhanced the yield of polygonal cells with CEC‑specific marker expression shown by ICC and RT-qPCR. This study demonstrates that mouse ESCs and iPSCs were induced and expressed CEC differentiation markers when subjected to a two‑step inducement process, suggesting that they are a promising resource for corneal endothelium failure replacement therapy in the future.
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1 

Li C, Yu H, Ma Y, et al: Germline-competent mouse-induced pluripotent stem cell lines generated on human fibroblasts without exogenous leukemia inhibitory factor. PloS One. 4:e67242009. View Article : Google Scholar : PubMed/NCBI

2 

Chen H, Fan X, Xia J, et al: Electrospun chitosan-graft-poly (epsilon-caprolactone)/poly(epsilon-caprolactone) nanofibrous scaffolds for retinal tissue engineering. Int J Nanomedicine. 6:453–461. 2011.

3 

Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e452001. View Article : Google Scholar : PubMed/NCBI

4 

Kang L and Gao S: Pluripotency of induced pluripotent stem cells. J Anim Sci Biotechnol. 3:52012. View Article : Google Scholar : PubMed/NCBI

5 

Grabel L: Prospects for pluripotent stem cell therapies: into the clinic and back to the bench. J Cell Biochem. 113:381–387. 2012. View Article : Google Scholar

6 

Sugawara T, Nishino K, Umezawa A and Akutsu H: Investigating cellular identity and manipulating cell fate using induced pluripotent stem cells. Stem Cell Res Ther. 3:82012.PubMed/NCBI

7 

Okita K, Yamakawa T, Matsumura Y, et al: An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells. 31:458–466. 2013. View Article : Google Scholar

8 

Bard JB, Hay ED and Meller SM: Formation of the endothelium of the avian cornea: a study of cell movement in vivo. Dev Biol. 42:334–361. 1975. View Article : Google Scholar : PubMed/NCBI

9 

Gage PJ, Rhoades W, Prucka SK and Hjalt T: Fate maps of neural crest and mesoderm in the mammalian eye. Invest Ophthalmol Vis Sci. 46:4200–4208. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Genis-Galvez JM: Role of the lens in the morphogenesis of the iris and cornea. Nature. 210:209–210. 1966. View Article : Google Scholar : PubMed/NCBI

11 

Yue W, Pi QM, Zhang WJ, et al: Platelet endothelial cell adhesion molecule-1, stage-specific embryonic antigen-1, and Flk-1 mark distinct populations of mouse embryonic stem cells during differentiation toward hematopoietic/endothelial cells. Stem Cells Dev. 19:1937–1948. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Li C, Wu Q, Liu B, et al: Detection and validation of circulating endothelial cells, a blood-based diagnostic marker of acute myocardial infarction. PLoS One. 8:e584782013. View Article : Google Scholar : PubMed/NCBI

13 

Yao Y, Wang L, Zhang H, et al: A novel anticancer therapy that simultaneously targets aberrant p53 and Notch activities in tumors. PLoS One. 7:e466272012. View Article : Google Scholar : PubMed/NCBI

14 

Akanuma H, Qin XY, Nagano R, et al: Identification of stage-specific gene expression signatures in response to retinoic acid during the neural differentiation of mouse embryonic stem cells. Front Genet. 3:1412012. View Article : Google Scholar : PubMed/NCBI

15 

Wang X, Wang J, Huang V, Place RF and Li LC: Induction of NANOG expression by targeting promoter sequence with small activating RNA antagonizes retinoic acid-induced differentiation. Biochem J. 443:821–828. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Paulsen BS, Souza CS, Chicaybam L, et al: Agathisflavone enhances retinoic acid-induced neurogenesis and its receptors alpha and beta in pluripotent stem cells. Stem Cells Dev. 20:1711–1721. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Kumar M, Bagchi B, Gupta SK, Meena AS, Gressens P and Mani S: Neurospheres derived from human embryoid bodies treated with retinoic acid show an increase in nestin and ngn2 expression that correlates with the proportion of tyrosine hydroxylase-positive cells. Stem Cells Dev. 16:667–681. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Prasad MS, Sauka-Spengler T and LaBonne C: Induction of the neural crest state: control of stem cell attributes by gene regulatory, post-transcriptional and epigenetic interactions. Dev Biol. 366:10–21. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Strobl-Mazzulla PH and Bronner ME: Epithelial to mesenchymal transition: new and old insights from the classical neural crest model. Semin Cancer Biol. 22:411–416. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Brafman DA, Phung C, Kumar N and Willert K: Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions. Cell Death Differ. 20:369–381. 2013. View Article : Google Scholar :

21 

Rowland TJ, Blaschke AJ, Buchholz DE, Hikita ST, Johnson LV and Clegg DO: Differentiation of human pluripotent stem cells to retinal pigmented epithelium in defined conditions using purified extracellular matrix proteins. J Tissue Eng Regen Med. 2012.PubMed/NCBI

22 

Shao C, Fu Y, Lu W and Fan X: Bone marrow-derived endothelial progenitor cells: a promising therapeutic alternative for corneal endothelial dysfunction. Cells Tissues Organs. 193:253–263. 2011. View Article : Google Scholar

23 

Ju C, Zhang K and Wu X: Derivation of corneal endothelial cell-like cells from rat neural crest cells in vitro. PLoS One. 7:e423782012. View Article : Google Scholar : PubMed/NCBI

24 

Ishizaki Y, Voyvodic JT, Burne JF and Raff MC: Control of lens epithelial cell survival. J Cell Biol. 121:899–908. 1993. View Article : Google Scholar : PubMed/NCBI

25 

Sugino IK, Rapista A, Sun Q, et al: A method to enhance cell survival on Bruch’s membrane in eyes affected by age and age-related macular degeneration. Invest Ophthalmol Vis Sci. 52:9598–9609. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Hatou S, Yoshida S, Higa K, et al: Functional corneal endothelium derived from corneal stroma stem cells of neural crest origin by retinoic acid and Wnt/beta-catenin signaling. Stem Cells Dev. 22:828–839. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Chen P, Chen JZ, Shao CY, Li CY, Zhang YD, Lu WJ, Fu Y, Gu P and Fan X: Treatment with retinoic acid and lens epithelial cell-conditioned medium in vitro directed the differentiation of pluripotent stem cells towards corneal endothelial cell-like cells. Exp Ther Med 9: 351-360, 2015.
APA
Chen, P., Chen, J., Shao, C., Li, C., Zhang, Y., Lu, W. ... Fan, X. (2015). Treatment with retinoic acid and lens epithelial cell-conditioned medium in vitro directed the differentiation of pluripotent stem cells towards corneal endothelial cell-like cells. Experimental and Therapeutic Medicine, 9, 351-360. https://doi.org/10.3892/etm.2014.2103
MLA
Chen, P., Chen, J., Shao, C., Li, C., Zhang, Y., Lu, W., Fu, Y., Gu, P., Fan, X."Treatment with retinoic acid and lens epithelial cell-conditioned medium in vitro directed the differentiation of pluripotent stem cells towards corneal endothelial cell-like cells". Experimental and Therapeutic Medicine 9.2 (2015): 351-360.
Chicago
Chen, P., Chen, J., Shao, C., Li, C., Zhang, Y., Lu, W., Fu, Y., Gu, P., Fan, X."Treatment with retinoic acid and lens epithelial cell-conditioned medium in vitro directed the differentiation of pluripotent stem cells towards corneal endothelial cell-like cells". Experimental and Therapeutic Medicine 9, no. 2 (2015): 351-360. https://doi.org/10.3892/etm.2014.2103
Copy and paste a formatted citation
x
Spandidos Publications style
Chen P, Chen JZ, Shao CY, Li CY, Zhang YD, Lu WJ, Fu Y, Gu P and Fan X: Treatment with retinoic acid and lens epithelial cell-conditioned medium in vitro directed the differentiation of pluripotent stem cells towards corneal endothelial cell-like cells. Exp Ther Med 9: 351-360, 2015.
APA
Chen, P., Chen, J., Shao, C., Li, C., Zhang, Y., Lu, W. ... Fan, X. (2015). Treatment with retinoic acid and lens epithelial cell-conditioned medium in vitro directed the differentiation of pluripotent stem cells towards corneal endothelial cell-like cells. Experimental and Therapeutic Medicine, 9, 351-360. https://doi.org/10.3892/etm.2014.2103
MLA
Chen, P., Chen, J., Shao, C., Li, C., Zhang, Y., Lu, W., Fu, Y., Gu, P., Fan, X."Treatment with retinoic acid and lens epithelial cell-conditioned medium in vitro directed the differentiation of pluripotent stem cells towards corneal endothelial cell-like cells". Experimental and Therapeutic Medicine 9.2 (2015): 351-360.
Chicago
Chen, P., Chen, J., Shao, C., Li, C., Zhang, Y., Lu, W., Fu, Y., Gu, P., Fan, X."Treatment with retinoic acid and lens epithelial cell-conditioned medium in vitro directed the differentiation of pluripotent stem cells towards corneal endothelial cell-like cells". Experimental and Therapeutic Medicine 9, no. 2 (2015): 351-360. https://doi.org/10.3892/etm.2014.2103
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