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Article

Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes

  • Authors:
    • Yuyu Chen
    • Baiping Wu
    • Liangzhuan Liu
    • Xiaoxing You
    • Lili Chen
    • Yimou Wu
    • Qiugui Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Inspection, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China, Department of Pathogenic Biology, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Inspection, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China
  • Pages: 459-463
    |
    Published online on: December 5, 2014
       https://doi.org/10.3892/etm.2014.2111
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Abstract

The aim of the present study was to express the recombinant Chlamydophila pneumoniae (C. pneumoniae) protein, Cpn 0810, in Escherichia coli (E. coli) BL21, and investigate the effects of Cpn 0810 on inflammatory and apoptotic processes in human monocytic (THP‑1) cells. An ELISA was performed to detect the levels of the proinflammatory cytokines, tumor necrosis factor (TNF)‑α and interleukin (IL)‑6. In addition, Hoechst 33258 staining and annexin V binding analyses were performed to measure the rates of apoptosis. Purified glutathione S‑transferase (GST)‑Cpn 0810 recombinant proteins were obtained from the E. coli BL21 cells carrying the pGEX6p‑2/Cpn 0810 plasmid, and were shown to stimulate the expression of TNF‑α and IL‑6 in the THP‑1 cells in a dose‑ and time‑dependent manner. TNF‑α and IL‑6 levels peaked at 24 h after GST‑Cpn 0810 stimulation. Furthermore, GST‑Cpn 0810 significantly promoted the apoptosis of THP‑1 cells. In conclusion, recombinant GST‑Cpn 0810 was shown to stimulate the expression of TNF‑α and IL‑6, inhibit proliferation and induce apoptosis in THP‑1 cells. Therefore, Cpn 0810 may interact with host cells following C. pneumoniae infection, functioning as an important pathogenic factor.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Wu B, Liu L, You X, Chen L, Wu Y and Zhang Q: Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes. Exp Ther Med 9: 459-463, 2015.
APA
Chen, Y., Wu, B., Liu, L., You, X., Chen, L., Wu, Y., & Zhang, Q. (2015). Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes. Experimental and Therapeutic Medicine, 9, 459-463. https://doi.org/10.3892/etm.2014.2111
MLA
Chen, Y., Wu, B., Liu, L., You, X., Chen, L., Wu, Y., Zhang, Q."Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes". Experimental and Therapeutic Medicine 9.2 (2015): 459-463.
Chicago
Chen, Y., Wu, B., Liu, L., You, X., Chen, L., Wu, Y., Zhang, Q."Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes". Experimental and Therapeutic Medicine 9, no. 2 (2015): 459-463. https://doi.org/10.3892/etm.2014.2111
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Wu B, Liu L, You X, Chen L, Wu Y and Zhang Q: Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes. Exp Ther Med 9: 459-463, 2015.
APA
Chen, Y., Wu, B., Liu, L., You, X., Chen, L., Wu, Y., & Zhang, Q. (2015). Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes. Experimental and Therapeutic Medicine, 9, 459-463. https://doi.org/10.3892/etm.2014.2111
MLA
Chen, Y., Wu, B., Liu, L., You, X., Chen, L., Wu, Y., Zhang, Q."Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes". Experimental and Therapeutic Medicine 9.2 (2015): 459-463.
Chicago
Chen, Y., Wu, B., Liu, L., You, X., Chen, L., Wu, Y., Zhang, Q."Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes". Experimental and Therapeutic Medicine 9, no. 2 (2015): 459-463. https://doi.org/10.3892/etm.2014.2111
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