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Article

Preventive effect of pentoxifylline on contrast‑induced acute kidney injury in hypercholesterolemic rats

  • Authors:
    • Shi‑Kun Yang
    • Shao‑Bin Duan
    • Peng Pan
    • Xiang‑Qing Xu
    • Na Liu
    • Jun Xu
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 384-388
    |
    Published online on: December 15, 2014
       https://doi.org/10.3892/etm.2014.2132
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Abstract

Oxidative stress is an important mechanism of contrast‑induced acute kidney injury (CIAKI). The optimal strategy to prevent CIAKI remains unclear. The aim of the present study was to assess the effect of pentoxifylline, a nonspecific phosphodiesterase inhibitor, on the prevention of CIAKI. A total of 32 healthy male Sprague‑Dawley rats were randomly divided into normal dietary group (NN; n=8) and a high cholesterol‑supplemented dietary group (HN; 4% cholesterol and 1% cholic acid; n=24). At the end of eight weeks, the rats in the high cholesterol diet group were randomly divided into three subgroups (n=8 in each group). CIAKI was induced in two of the subgroups via intravenous injection of the radiocontrast media iohexol (10 ml/kg). Pentoxifylline (50 mg/kg) was administered to one of the iohexol‑treated groups via intraperitoneal injection 12 h prior to and following contrast media (CM) injection. Kidney function parameters and oxidative stress markers were then measured. The renal pathological changes were evaluated using hematoxylin and eosin staining and scored semi‑quantitatively. In iohexol‑injected rats, serum creatinine (Scr), renal pathological scores, renal malondialdehyde (MDA) content, renal NADPH oxidase activity, fractional excretion of sodium (FENa%) and fractional excretion of potassium (FEK%) were significantly increased (P<0.01). The Scr, histologic scores, renal MDA content, NADPH oxidase activity, FENa% and FEK% in the rats treated with pentoxifylline prior to iohexol were observed to be reduced compared with those in rats treated with iohexol alone (P<0.01). This suggests that pentoxifylline significantly attenuates renal injuries, including tubular necrosis and proteinaceous casts induced by CM. It may be concluded that pentoxifylline protected the renal tissue from the nephrotoxicity induced by low‑osmolar CM via an antioxidant effect.
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Copy and paste a formatted citation
Spandidos Publications style
Yang SK, Duan SB, Pan P, Xu XQ, Liu N and Xu J: Preventive effect of pentoxifylline on contrast‑induced acute kidney injury in hypercholesterolemic rats. Exp Ther Med 9: 384-388, 2015.
APA
Yang, S., Duan, S., Pan, P., Xu, X., Liu, N., & Xu, J. (2015). Preventive effect of pentoxifylline on contrast‑induced acute kidney injury in hypercholesterolemic rats. Experimental and Therapeutic Medicine, 9, 384-388. https://doi.org/10.3892/etm.2014.2132
MLA
Yang, S., Duan, S., Pan, P., Xu, X., Liu, N., Xu, J."Preventive effect of pentoxifylline on contrast‑induced acute kidney injury in hypercholesterolemic rats". Experimental and Therapeutic Medicine 9.2 (2015): 384-388.
Chicago
Yang, S., Duan, S., Pan, P., Xu, X., Liu, N., Xu, J."Preventive effect of pentoxifylline on contrast‑induced acute kidney injury in hypercholesterolemic rats". Experimental and Therapeutic Medicine 9, no. 2 (2015): 384-388. https://doi.org/10.3892/etm.2014.2132
Copy and paste a formatted citation
x
Spandidos Publications style
Yang SK, Duan SB, Pan P, Xu XQ, Liu N and Xu J: Preventive effect of pentoxifylline on contrast‑induced acute kidney injury in hypercholesterolemic rats. Exp Ther Med 9: 384-388, 2015.
APA
Yang, S., Duan, S., Pan, P., Xu, X., Liu, N., & Xu, J. (2015). Preventive effect of pentoxifylline on contrast‑induced acute kidney injury in hypercholesterolemic rats. Experimental and Therapeutic Medicine, 9, 384-388. https://doi.org/10.3892/etm.2014.2132
MLA
Yang, S., Duan, S., Pan, P., Xu, X., Liu, N., Xu, J."Preventive effect of pentoxifylline on contrast‑induced acute kidney injury in hypercholesterolemic rats". Experimental and Therapeutic Medicine 9.2 (2015): 384-388.
Chicago
Yang, S., Duan, S., Pan, P., Xu, X., Liu, N., Xu, J."Preventive effect of pentoxifylline on contrast‑induced acute kidney injury in hypercholesterolemic rats". Experimental and Therapeutic Medicine 9, no. 2 (2015): 384-388. https://doi.org/10.3892/etm.2014.2132
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