Open Access

Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin

  • Authors:
    • Hai‑Cheng Gao
    • Kun Zhu
    • Hai‑Mei Gao
    • Chun‑Sheng Miao
    • Le‑Ning Zhang
    • Wei Liu
    • Hua Xin
  • View Affiliations

  • Published online on: January 28, 2015     https://doi.org/10.3892/etm.2015.2223
  • Pages: 1103-1108
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of this study was to investigate the role of tissue transglutaminase (tTG) in the pathogenesis of diabetic cardiomyopathy (DCM) and the intervention effect of rutin. DCM was induced in rats by the injection of streptozotocin (STZ; 25 mg/kg). After a preliminary examination, the rats were randomly divided into four groups: Control (n=8), STZ‑induced DCM (n=8), STZ + positive drug (captopril; n=6) and STZ + rutin (n=8) groups. The DCM model was evaluated using blood sugar values, serum enzyme levels, hematoxylin and eosin staining and Masson's staining, ex vivo. The protein and mRNA expression of tTG was assessed with immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The rat model of DCM was successfully established by STZ administration, and the expression levels of tTG were significantly increased in the DCM model. Following the injection of captopril or rutin, the blood sugar values, collagen content and expression levels of tTG were gradually reduced and serum enzyme levels were increased, as compared with those in the STZ‑induced DCM group. In conclusion, tTG plays an important role in STZ‑induced DCM. In addition, rutin may inhibit the expression of tTG and regulate myocardial injury in STZ‑induced DCM.
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April-2015
Volume 9 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gao HC, Zhu K, Gao HM, Miao CS, Zhang LN, Liu W and Xin H: Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin. Exp Ther Med 9: 1103-1108, 2015
APA
Gao, H., Zhu, K., Gao, H., Miao, C., Zhang, L., Liu, W., & Xin, H. (2015). Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin. Experimental and Therapeutic Medicine, 9, 1103-1108. https://doi.org/10.3892/etm.2015.2223
MLA
Gao, H., Zhu, K., Gao, H., Miao, C., Zhang, L., Liu, W., Xin, H."Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin". Experimental and Therapeutic Medicine 9.4 (2015): 1103-1108.
Chicago
Gao, H., Zhu, K., Gao, H., Miao, C., Zhang, L., Liu, W., Xin, H."Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1103-1108. https://doi.org/10.3892/etm.2015.2223