Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin

Gao,Hai‑Cheng; Zhu,Kun; Gao,Hai‑Mei; Miao,Chun‑Sheng; Zhang,Le‑Ning; Liu,Wei; Xin,Hua

doi:10.3892/etm.2015.2223

April-2015 Volume 9 Issue 4

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

April-2015 Volume 9 Issue 4

Full Size Image

Article Open Access

Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin

Authors:
- Hai‑Cheng Gao
- Kun Zhu
- Hai‑Mei Gao
- Chun‑Sheng Miao
- Le‑Ning Zhang
- Wei Liu
- Hua Xin
View Affiliations / Copyright

Affiliations: Department of Clinical Pharmacy and Pharmaceutical Management, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Endocrinology, The 208th Hospital of PLA, Changchun, Jilin 130000, P.R. China, Patient's Information Recording Room, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Chest Surgery, The China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130000, P.R. China

Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1103-1108
|
Published online on: January 28, 2015

https://doi.org/10.3892/etm.2015.2223
Expand metrics +

Abstract

The aim of this study was to investigate the role of tissue transglutaminase (tTG) in the pathogenesis of diabetic cardiomyopathy (DCM) and the intervention effect of rutin. DCM was induced in rats by the injection of streptozotocin (STZ; 25 mg/kg). After a preliminary examination, the rats were randomly divided into four groups: Control (n=8), STZ‑induced DCM (n=8), STZ + positive drug (captopril; n=6) and STZ + rutin (n=8) groups. The DCM model was evaluated using blood sugar values, serum enzyme levels, hematoxylin and eosin staining and Masson's staining, ex vivo. The protein and mRNA expression of tTG was assessed with immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The rat model of DCM was successfully established by STZ administration, and the expression levels of tTG were significantly increased in the DCM model. Following the injection of captopril or rutin, the blood sugar values, collagen content and expression levels of tTG were gradually reduced and serum enzyme levels were increased, as compared with those in the STZ‑induced DCM group. In conclusion, tTG plays an important role in STZ‑induced DCM. In addition, rutin may inhibit the expression of tTG and regulate myocardial injury in STZ‑induced DCM.

View Figures

View References

1	Pelle AJ, Loerbroks A, Widdershoven JW and Denollet J: Heart failure and comorbid diabetes mellitus or chronic obstructive pulmonary disease: effects on mood in outpatients. Int J Cardiol. 154:216–217. 2012. View Article : Google Scholar
2	Li B, Zheng Z, Wei Y, et al: Therapeutic effects of neuregulin-1 in diabetic cardiomyopathy rats. Cardiovasc Diabetol. 10:692011. View Article : Google Scholar : PubMed/NCBI
3	Asghar O, Al-Sunni A, Khavandi K, et al: Diabetic cardiomyopathy. Clin Sci (Lond). 116:741–760. 2009. View Article : Google Scholar
4	Avogaro A, Vigili de Kreutzenberg S, Negut C, Tiengo A and Scognamiglio R: Diabetic cardiomyopathy: a metabolic perspective. Am J Cardiol. 93:13A–16A. 2004. View Article : Google Scholar : PubMed/NCBI
5	Lee SW, Song KE, Shin DS, et al: Alterations in peripheral blood levels of TIMP-1, MMP-2, and MMP-9 in patients with type-2 diabetes. Diabetes Res Clin Pract. 69:175–9. 2005. View Article : Google Scholar : PubMed/NCBI
6	Tucholski J and Johnson GV: Tissue transglutaminase differentially modulates apoptosis in a stimuli-dependent manner. J Neurochem. 81:780–791. 2002. View Article : Google Scholar : PubMed/NCBI
7	Barsigian C, Stern AM and Martinez J: Tissue (type II) transglutaminase covalently incorporates itself, fibrinogen, or fibronectin into high molecular weight complexes on the extracellular surface of isolated hepatocytes. Use of 2-[(2-oxopropyl)thio] imidazolium derivatives as cellular transglutaminase inactivators. J Biol Chem. 266:22501–22509. 1991.PubMed/NCBI
8	Song H, Kim BK, Chang W, et al: Tissue transglutaminase 2 promotes apoptosis of rat neonatal cardiomyocytes under oxidative stress. J Recept Signal Transduct Res. 31:66–74. 2011. View Article : Google Scholar
9	Lauzier A, Charbonneau M, Paquette M, Harper K and Dubois CM: Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis. Arthritis Res Ther. 14:R1592012. View Article : Google Scholar : PubMed/NCBI
10	Zhang Z, Wang S, Zhou S, et al: Sulforaphane prevents the development of cardiomyopathy in type 2 diabetic mice probably by reversing oxidative stress-induced inhibition of LKB1/AMPK pathway. J Mol Cell Cardiol. Sep 28–2014.(Epub ahead of print). View Article : Google Scholar
11	Kappel VD, Cazarolli LH, Pereira DF, et al: Involvement of GLUT-4 in the stimulatory effect of rutin on glucose uptake in rat soleus muscle. J Pharm Pharmacol. 65:1179–1186. 2013. View Article : Google Scholar : PubMed/NCBI
12	Boudina S and Abel ED: Diabetic cardiomyopathy revisited. Circulation. 115:3213–3223. 2007. View Article : Google Scholar : PubMed/NCBI
13	Mäki M: Tissue transglutaminase as the autoantigen of coeliac disease. Gut. 41:565–566. 1997. View Article : Google Scholar : PubMed/NCBI
14	Liao CW, Fan CK, Kao TC, et al: Brain injury-associated biomarkers of TGF-beta1, S100B, GFAP, NF-L, tTG, AbetaPP, and tau were concomitantly enhanced and the UPS was impaired during acute brain injury caused by Toxocara canis in mice. BMC Infect Dis. 8:842008. View Article : Google Scholar : PubMed/NCBI
15	Petrak J, Pospisilova J, Sedinova M, et al: Proteomic and transcriptomic analysis of heart failure due to volume overload in a rat aorto-caval fistula model provides support for new potential therapeutic targets - monoamine oxidase A and transglutaminase 2. Proteome Sci. 9:692011. View Article : Google Scholar : PubMed/NCBI
16	Mirza A, Liu SL, Frizell E, et al: A role for tissue transglutaminase in hepatic injury and fibrogenesis, and its regulation by NF-kappaB. Am J Physiol. 272:G281–G288. 1997.PubMed/NCBI
17	Hao HH, Shao ZM, Tang DQ, et al: Preventive effects of rutin on the development of experimental diabetic nephropathy in rats. Life Sci. 91:959–967. 2012. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Related Articles