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Article

Effects of bone marrow‑derived mesenchymal stem cells transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis

  • Authors:
    • Ying‑Ming Song
    • Chang‑Hong Lian
    • Cheng‑Song Wu
    • Ai‑Fang Ji
    • Juan‑Juan Xiang
    • Xiao‑Yan Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Heping Hospital, Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China, Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China
  • Pages: 1292-1298
    |
    Published online on: January 29, 2015
       https://doi.org/10.3892/etm.2015.2232
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Abstract

The aim of the present study was to compare the effects of bone marrow‑derived mesenchymal stem cells (BMSCs) transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis. BMSCs were isolated from rat bone marrow and labeled with green fluorescent protein (GFP). Then, the labeled BMSCs were injected into rats with liver injury via the portal vein or tail vein. Two weeks after transplantation, three rats in each group were sacrificed to test the distribution of GFP in the liver and the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and albumin. Six weeks later, the remaining rats were sacrificed, and serum ALT, AST, albumin, hyaluronic acid (HA), laminin (LN) and procollagen type III (PC‑III) levels were measured. The expression of albumin in the liver was analyzed by immunohistochemistry. Two weeks after BMSC transplantation, GFP‑positive cells were detected in the livers of rats with BMSCs transplanted via the portal vein and tail vein. Compared with pre‑transplantation levels, the ALT levels of the groups with BMSC transplantation via the portal vein and tail vein were significantly decreased after two and six weeks of BMSC transplantation (P<0.05), whereas the AST and albumin levels were not significantly different at two weeks after BMSC transplantation in the two groups (all P>0.05). However, the AST and albumin levels were significantly reduced at six weeks after BMSC transplantation (all P<0.05). At six weeks after BMSC transplantation, the serum HA, LN and PC‑III levels in rats transplanted with BMSCs via the portal vein or tail vein had decreased significantly (all P<0.05), as compared with the levels prior to BMSC transplantation. BMSCs transplanted via the portal vein and tail vein achieved similar improvements in liver function in rats with liver cirrhosis, which suggests that peripheral venous administration is a convenient and effective route for BMSC transplantation.
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Copy and paste a formatted citation
Spandidos Publications style
Song YM, Lian CH, Wu CS, Ji AF, Xiang JJ and Wang XY: Effects of bone marrow‑derived mesenchymal stem cells transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis. Exp Ther Med 9: 1292-1298, 2015.
APA
Song, Y., Lian, C., Wu, C., Ji, A., Xiang, J., & Wang, X. (2015). Effects of bone marrow‑derived mesenchymal stem cells transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis. Experimental and Therapeutic Medicine, 9, 1292-1298. https://doi.org/10.3892/etm.2015.2232
MLA
Song, Y., Lian, C., Wu, C., Ji, A., Xiang, J., Wang, X."Effects of bone marrow‑derived mesenchymal stem cells transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis". Experimental and Therapeutic Medicine 9.4 (2015): 1292-1298.
Chicago
Song, Y., Lian, C., Wu, C., Ji, A., Xiang, J., Wang, X."Effects of bone marrow‑derived mesenchymal stem cells transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1292-1298. https://doi.org/10.3892/etm.2015.2232
Copy and paste a formatted citation
x
Spandidos Publications style
Song YM, Lian CH, Wu CS, Ji AF, Xiang JJ and Wang XY: Effects of bone marrow‑derived mesenchymal stem cells transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis. Exp Ther Med 9: 1292-1298, 2015.
APA
Song, Y., Lian, C., Wu, C., Ji, A., Xiang, J., & Wang, X. (2015). Effects of bone marrow‑derived mesenchymal stem cells transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis. Experimental and Therapeutic Medicine, 9, 1292-1298. https://doi.org/10.3892/etm.2015.2232
MLA
Song, Y., Lian, C., Wu, C., Ji, A., Xiang, J., Wang, X."Effects of bone marrow‑derived mesenchymal stem cells transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis". Experimental and Therapeutic Medicine 9.4 (2015): 1292-1298.
Chicago
Song, Y., Lian, C., Wu, C., Ji, A., Xiang, J., Wang, X."Effects of bone marrow‑derived mesenchymal stem cells transplanted via the portal vein or tail vein on liver injury in rats with liver cirrhosis". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1292-1298. https://doi.org/10.3892/etm.2015.2232
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