Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
April-2015 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2015 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Blockade of Plasmodium falciparum erythrocyte invasion: New assessment of anti-Plasmodium falciparum reticulocyte‑binding protein homolog 5 antibodies

  • Authors:
    • Yan Shen
    • Jun Wang
    • Xuewu Liu
    • Jiao Liang
    • Yuxiao Huang
    • Zhongxiang Liu
    • Ya Zhao
    • Yinghui Li
  • View Affiliations / Copyright

    Affiliations: Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 1357-1362
    |
    Published online on: January 29, 2015
       https://doi.org/10.3892/etm.2015.2237
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

There is great interest in any new discoveries in malaria vaccine research. Plasmodium falciparum reticulocyte‑binding protein homolog 5 (PfRH5) shows promise in this area and may be used together with other merozoite antigens as a potential vaccine. In the present study, a bioinformatics prediction approach was applied to a PfRH5 B‑cell epitope, and two B‑cell epitope distributions were selected. Antibodies against the two PfRH5 distributions were obtained and the growth activity inhibition was measured. No inhibition of the P. falciparum CY strain was found, but the growth of the P. falciparum 3D7 strain was inhibited by all of the antibodies, in contrast to the results of other studies. It was additionally found that certain quantities of protein led to the inhibition of the parasitic invasion. Equally noteworthy was that the survival time of the group immunized with a portion of PfRH5 was significantly longer than that of the group immunized with the full‑length protein, following infection by P. berghei ANKA. The present study produced conflicting results in in vitro and in vivo experiments, although the accuracy of the evaluation may be lessened due to the use of a murine malaria model. The findings of the present study may indicate that PfRH5 may not be suitable in malaria vaccine research.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Agnandji ST, Lell B, Soulanoudjingar SS, Fernandes JF, Abossolo BP, Conzelmann C, Methogo BG, Doucka Y, Flamen A, Mordmüller B, et al; RTS,S Clinical Trials Partnership. First results of phase 3 trial of RTS,S/AS01 malaria vaccine in African children. N Engl J Med. 365:1863–1875. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Tolia NH, Enemark EJ, Sim BK and Joshua-Tor L: Structural basis for the EBA-175 erythrocyte invasion pathway of the malaria parasite Plasmodium falciparum. Cell. 122:183–193. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Jaśkiewicz E, Graczyk J and Rydzak J: Proteins involved in invasion of human red blood cells by malaria parasites. Postepy Hig Med Dosw (Online). 64:617–626. 2010.(In Polish).

4 

Lopaticki S, Maier AG, Thompson J, Wilson DW, Tham WH, Triglia T, Gout A, Speed TP, Beeson JG, Healer J and Cowman AF: Reticulocyte and erythrocyte binding-like proteins function cooperatively in invasion of human erythrocytes by malaria parasites. Infect Immun. 79:1107–1117. 2011. View Article : Google Scholar :

5 

Nolan T: Identifying an essential interaction between malaria parasites and erythrocytes unlocks the door to promising vaccine targets. Pathog Glob Health. 106:642012. View Article : Google Scholar : PubMed/NCBI

6 

Douglas AD, Williams AR, Illingworth JJ, Kamuyu G, Biswas S, Goodman AL, Wyllie DH, Crosnier C, Miura K, Wright GJ, et al: The blood-stage malaria antigen PfRH5 is susceptible to vaccine-inducible cross-strain neutralizing antibody. Nat Commun. 2:6012011. View Article : Google Scholar : PubMed/NCBI

7 

Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner JC and Wright GJ: Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. Nature. 480:534–537. 2011.PubMed/NCBI

8 

Muramatsu T: Basigin: a multifunctional membrane protein with an emerging role in infections by malaria parasites. Expert Opin Ther Targets. 16:999–1011. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Trager W and Jensen JB: Human malaria parasites in continuous culture. 1976. J Parasitol. 91:484–486. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Cui L, Rzomp KA, Fan Q, Martin SK and Williams J: Plasmodium falciparum: differential display analysis of gene expression during gametocytogenesis. Exp Parasitol. 99:244–254. 2001. View Article : Google Scholar

11 

Jameson BA and Wolf H: The antigenic index: a novel algorithm for predicting antigenic determinants. Comput Appl Biosci. 4:181–186. 1988.PubMed/NCBI

12 

Garnier J, Osguthorpe DJ and Robson B: Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol. 120:97–120. 1978. View Article : Google Scholar : PubMed/NCBI

13 

Creighton TE: Prediction of Protein Structure and the Principles of Protein Conformation. Fasman Gerald D: Plenum; New York: 1989, pp. xivpp. 798illus $95. Science. 247. pp. 1351–1352. 1990

14 

Karplus PA and Schulz GE: Prediction of chain flexibility in proteins. Naturwissenschaften. 72:212–213. 1985. View Article : Google Scholar

15 

Baum J, Chen L, Healer J, Lopaticki S, Boyle M, Triglia T, Ehlgen F, Ralph SA, Beeson JG and Cowman AF: Reticulocyte-binding protein homologue 5 - an essential adhesin involved in invasion of human erythrocytes by Plasmodium falciparum. Int J Parasitol. 39:371–380. 2009. View Article : Google Scholar

16 

Wahlgren M, Berzins K, Perlmann P and Björkman A: Characterization of the humoral immune response in Plasmodium falciparum malaria. I Estimation of antibodies to P falciparum or human erythrocytes by means of microELISA. Clin Exp Immunol. 54:127–134. 1983.PubMed/NCBI

17 

Miao J, Li X, Liu Z, Xue C, Bujard H and Cui L: Immune responses in mice induced by prime-boost schemes of the Plasmodium falciparum apical membrane antigen 1 (PfAMA1)-based DNA, protein and recombinant modified vaccinia Ankara vaccines. Vaccine. 24:6187–6198. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Jogdand PS, Singh SK, Christiansen M, Dziegiel MH, Singh S and Theisen M: Flow cytometric readout based on Mitotracker Red CMXRos staining of live asexual blood stage malarial parasites reliably assesses antibody dependent cellular inhibition. Malar J. 11:2352012. View Article : Google Scholar : PubMed/NCBI

19 

Taylor HM, Grainger M and Holder AA: Variation in the expression of a Plasmodium falciparum protein family implicated in erythrocyte invasion. Infect Immun. 70:5779–5789. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Duraisingh MT, Triglia T, Ralph SA, Rayner JC, Barnwell JW, McFadden GI and Cowman AF: Phenotypic variation of Plasmodium falciparum merozoite proteins directs receptor targeting for invasion of human erythrocytes. EMBO J. 22:1047–1057. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Nery S, Deans AM, Mosobo M, Marsh K, Rowe JA and Conway DJ: Expression of Plasmodium falciparum genes involved in erythrocyte invasion varies among isolates cultured directly from patients. Mol Biochem Parasitol. 149:208–215. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Hayton K, Gaur D, Liu A, Takahashi J, Henschen B, Singh S, Lambert L, Furuya T, Bouttenot R, Doll M, et al: Erythrocyte binding protein PfRH5 polymorphisms determine species-specific pathways of Plasmodium falciparum invasion. Cell Host Microbe. 4:40–51. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Rodriguez M, Lustigman S, Montero E, Oksov Y and Lobo CA: PfRH5: a novel reticulocyte-binding family homolog of Plasmodium falciparum that binds to the erythrocyte, and an investigation of its receptor. PloS One. 3:e33002008. View Article : Google Scholar : PubMed/NCBI

24 

Rayner JC, Galinski MR, Ingravallo P and Barnwell JW: Two Plasmodium falciparum genes express merozoite proteins that are related to Plasmodium vivax and Plasmodium yoelii adhesive proteins involved in host cell selection and invasion. Proc Natl Acad Sci USA. 97:9648–9653. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Triglia T, Duraisingh MT, Good RT and Cowman AF: Reticulocyte-binding protein homologue 1 is required for sialic acid-dependent invasion into human erythrocytes by Plasmodium falciparum. Mol Microbiol. 55:162–174. 2005. View Article : Google Scholar

26 

Stubbs J, Simpson KM, Triglia T, Plouffe D, Tonkin CJ, Duraisingh MT, Maier AG, Winzeler EA and Cowman AF: Molecular mechanism for switching of P. falciparum invasion pathways into human erythrocytes. Science. 309:1384–1387. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Bustamante LY, Bartholdson SJ, Crosnier C, Campos MG, Wanaguru M, Nguon C, Kwiatkowski DP, Wright GJ and Rayner JC: A full-length recombinant Plasmodium falciparum PfRH5 protein induces inhibitory antibodies that are effective across common PfRH5 genetic variants. Vaccine. 31:373–379. 2013. View Article : Google Scholar :

28 

Williams AR, Douglas AD, Miura K, Illingworth JJ, Choudhary P, Murungi LM, Furze JM, Diouf A, Miotto O, Crosnier C, et al: Enhancing blockade of Plasmodium falciparum erythrocyte invasion: assessing combinations of antibodies against PfRH5 and other merozoite antigens. PLoS Pathog. 8:e10029912012. View Article : Google Scholar : PubMed/NCBI

29 

Lopez-Perez M, Villasis E, Machado RL, Póvoa MM, Vinetz JM, Blair S, Gamboa D and Lustigman S: Plasmodium falciparum field isolates from South America use an atypical red blood cell invasion pathway associated with invasion ligand polymorphisms. PLoS One. 7:e479132012. View Article : Google Scholar : PubMed/NCBI

30 

Villasis E, Lopez-Perez M, Torres K, Gamboa D, Neyra V, Bendezu J, Tricoche N, Lobo C, Vinetz JM and Lustigman S: Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru. Malar J. 11:3612012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shen Y, Wang J, Liu X, Liang J, Huang Y, Liu Z, Zhao Y and Li Y: Blockade of Plasmodium falciparum erythrocyte invasion: New assessment of anti-Plasmodium falciparum reticulocyte‑binding protein homolog 5 antibodies. Exp Ther Med 9: 1357-1362, 2015.
APA
Shen, Y., Wang, J., Liu, X., Liang, J., Huang, Y., Liu, Z. ... Li, Y. (2015). Blockade of Plasmodium falciparum erythrocyte invasion: New assessment of anti-Plasmodium falciparum reticulocyte‑binding protein homolog 5 antibodies. Experimental and Therapeutic Medicine, 9, 1357-1362. https://doi.org/10.3892/etm.2015.2237
MLA
Shen, Y., Wang, J., Liu, X., Liang, J., Huang, Y., Liu, Z., Zhao, Y., Li, Y."Blockade of Plasmodium falciparum erythrocyte invasion: New assessment of anti-Plasmodium falciparum reticulocyte‑binding protein homolog 5 antibodies". Experimental and Therapeutic Medicine 9.4 (2015): 1357-1362.
Chicago
Shen, Y., Wang, J., Liu, X., Liang, J., Huang, Y., Liu, Z., Zhao, Y., Li, Y."Blockade of Plasmodium falciparum erythrocyte invasion: New assessment of anti-Plasmodium falciparum reticulocyte‑binding protein homolog 5 antibodies". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1357-1362. https://doi.org/10.3892/etm.2015.2237
Copy and paste a formatted citation
x
Spandidos Publications style
Shen Y, Wang J, Liu X, Liang J, Huang Y, Liu Z, Zhao Y and Li Y: Blockade of Plasmodium falciparum erythrocyte invasion: New assessment of anti-Plasmodium falciparum reticulocyte‑binding protein homolog 5 antibodies. Exp Ther Med 9: 1357-1362, 2015.
APA
Shen, Y., Wang, J., Liu, X., Liang, J., Huang, Y., Liu, Z. ... Li, Y. (2015). Blockade of Plasmodium falciparum erythrocyte invasion: New assessment of anti-Plasmodium falciparum reticulocyte‑binding protein homolog 5 antibodies. Experimental and Therapeutic Medicine, 9, 1357-1362. https://doi.org/10.3892/etm.2015.2237
MLA
Shen, Y., Wang, J., Liu, X., Liang, J., Huang, Y., Liu, Z., Zhao, Y., Li, Y."Blockade of Plasmodium falciparum erythrocyte invasion: New assessment of anti-Plasmodium falciparum reticulocyte‑binding protein homolog 5 antibodies". Experimental and Therapeutic Medicine 9.4 (2015): 1357-1362.
Chicago
Shen, Y., Wang, J., Liu, X., Liang, J., Huang, Y., Liu, Z., Zhao, Y., Li, Y."Blockade of Plasmodium falciparum erythrocyte invasion: New assessment of anti-Plasmodium falciparum reticulocyte‑binding protein homolog 5 antibodies". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1357-1362. https://doi.org/10.3892/etm.2015.2237
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team