Curcumin improves the paclitaxel‑induced apoptosis of HPV‑positive human cervical cancer cells via the NF‑κB‑p53‑caspase‑3 pathway

  • Authors:
    • Yu-Ping Dang
    • Xiao-Ying Yuan
    • Rong Tian
    • Dong-Guang Li
    • Wei Liu
  • View Affiliations

  • Published online on: January 29, 2015     https://doi.org/10.3892/etm.2015.2240
  • Pages: 1470-1476
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Abstract

Paclitaxel, isolated from Taxus brevifolia, is considered to be an efficacious agent against a wide spectrum of human cancers, including human cervical cancer. However, dose‑limiting toxicity and high cost limit its clinical application. Curcumin, a nontoxic food additive, has been reported to improve paclitaxel chemotherapy in mouse models of cervical cancer. However, the underlying mechanisms remain unclear. In this study, two human cervical cancer cell lines, CaSki [human papilloma virus (HPV)16‑positive] and HeLa (HPV18‑positive), were selected in which to investigate the effect of curcumin on the anticancer action of paclitaxel and further clarify the mechanisms. Flow cytometry and MTT analysis demonstrated that curcumin significantly promoted paclitaxel‑induced apoptosis and cytotoxicity in the two cervical cell lines compared with that observed with paclitaxel alone (P<0.05). Reverse transcription‑polymerase chain reaction indicated that the decline of HPV E6 and E7 gene expression induced by paclitaxel was also assisted by curcumin. The expression levels of p53 protein and cleaved caspase‑3 were increased significantly in the curcumin plus paclitaxel‑treated HeLa and CaSki cells compared with those in the cells treated with paclitaxel alone (P<0.01). Significant reductions in the levels of phosphorylation of IκBα and the p65‑NF‑κB subunit in CaSki cells treated with curcumin and paclitaxel were observed compared with those in cells treated with paclitaxel alone (P<0.05). This suggests that the combined effect of curcumin and paclitaxel was associated with the NF‑κB‑p53‑caspase‑3 pathway. In conclusion, curcumin has the ability to improve the paclitaxel‑induced apoptosis of HPV‑positive human cervical cancer cell lines via the NF‑κB‑p53‑caspase‑3 pathway. Curcumin in combination with paclitaxel may provide a superior therapeutic effect on human cervical cancer.
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April-2015
Volume 9 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Dang Y, Yuan X, Tian R, Li D and Liu W: Curcumin improves the paclitaxel‑induced apoptosis of HPV‑positive human cervical cancer cells via the NF‑κB‑p53‑caspase‑3 pathway. Exp Ther Med 9: 1470-1476, 2015
APA
Dang, Y., Yuan, X., Tian, R., Li, D., & Liu, W. (2015). Curcumin improves the paclitaxel‑induced apoptosis of HPV‑positive human cervical cancer cells via the NF‑κB‑p53‑caspase‑3 pathway. Experimental and Therapeutic Medicine, 9, 1470-1476. https://doi.org/10.3892/etm.2015.2240
MLA
Dang, Y., Yuan, X., Tian, R., Li, D., Liu, W."Curcumin improves the paclitaxel‑induced apoptosis of HPV‑positive human cervical cancer cells via the NF‑κB‑p53‑caspase‑3 pathway". Experimental and Therapeutic Medicine 9.4 (2015): 1470-1476.
Chicago
Dang, Y., Yuan, X., Tian, R., Li, D., Liu, W."Curcumin improves the paclitaxel‑induced apoptosis of HPV‑positive human cervical cancer cells via the NF‑κB‑p53‑caspase‑3 pathway". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1470-1476. https://doi.org/10.3892/etm.2015.2240