Seawater immersion aggravates sciatic nerve injury in rats

Wang,Haifeng; Fang,Jian; Hu,Feng; Li,Gewei; Hong,He

doi:10.3892/etm.2015.2281

Seawater immersion aggravates sciatic nerve injury in rats

Authors:
- Haifeng Wang
- Jian Fang
- Feng Hu
- Gewei Li
- He Hong
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Affiliations: Department of Micro‑Orthopedics, The People's Liberation Army Clinical College Affiliated with Anhui Medical University (The 105th Hospital of People's Liberation Army), Hefei, Anhui 230031, P.R. China, Department of Orthopedics, The People's Liberation Army Clinical College Affiliated with Anhui Medical University (The 105th Hospital of People's Liberation Army), Hefei, Anhui 230031, P.R. China
Published online on: February 9, 2015 https://doi.org/10.3892/etm.2015.2281
Pages: 1153-1160
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was investigate the impact of seawater immersion on peripheral nerve injury and the underlying mechanisms. A total of 234 specific pathogen‑free Sprague‑Dawley male rats were randomly divided into a sham group, injury control group and seawater immersion + injury group. The Sciatic Functional Index (SFI) was used to assess nerve function for 6 weeks after injury. Compound muscle action potentials were measured and hematoxylin and eosin (H&E) staining of nerve specimens was carried out at week 6. Levels of reactive oxygen species (ROS) and malondialdehyde (MDA) in nerve tissues were measured by enzyme‑linked immunosorbent assay (ELISA), and the expression levels of inducible nitric oxide synthase (iNOS) mRNA and protein were measured by reverse transcription‑quantitative polymerase chain reaction and immunohistochemistry, respectively. The SFI value in the seawater immersion + injury group after 6 weeks was lower than that in the injury control group (P<0.05). The compound muscle action potential in the seawater immersion + injury group had a prolonged latency, and the amplitude and nerve conduction velocity were decreased compared with those in the other groups (P<0.05). H&E staining demonstrated that nerve fiber regeneration was worse in the seawater immersion + injury group. The ROS and MDA levels in the seawater immersion + injury group were higher than those in the other groups (P<0.05). The expression levels of iNOS mRNA and protein gradually increased in the injury and seawater immersion + injury groups and peaked at 48 h after surgery. Immersion in seawater further aggravated sciatic nerve injury and led to worse neuronal recovery. The mechanism may be associated with oxidative stress.

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