Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow

Shima,Wan  Nazatul; Ali,Abdul  Manaf; Subramani,Tamilselvan; Mohamed Alitheen,Noorjahan   Banu; Hamid,Muhajir; Samsudin,Abdul  Rani; Yeap,Swee  Keong

doi:10.3892/etm.2015.2381

June-2015 Volume 9 Issue 6

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June-2015 Volume 9 Issue 6

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Article

Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow

Authors:
- Wan Nazatul Shima
- Abdul Manaf Ali
- Tamilselvan Subramani
- Noorjahan Banu Mohamed Alitheen
- Muhajir Hamid
- Abdul Rani Samsudin
- Swee Keong Yeap
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Affiliations: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia, Faculty of Agriculture and Biotechnology, Universiti Zainal Abidin, Kampus Kota, Kuala Terengganu, Terengganu 20400, Malaysia, Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia, School of Dental Sciences, Universiti Sains Malaysia Health Campus, Kubang Kerian, Kelantan 16150, Malaysia, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia
Pages: 2202-2206
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Published online on: March 23, 2015

https://doi.org/10.3892/etm.2015.2381
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Abstract

Mesenchymal stem cells (MSCs) are involved in bone formation in the embryo, bone repair and remodeling. The differentiation of these cells is a complex multistep pathway that involves discrete cellular transitions and is similar to that which occurs during hematopoiesis. MSCs have self‑renewal capacity without differentiation in long‑term culture. In the present study, MSCs were isolated from human bone marrow and characterized by the presence of cluster of differentiation 105 marker using the labeled streptavidin biotin method. The MSCs were cultured in Dulbecco's modified Eagle's medium supplemented with fetal bovine serum, ascorbic acid, β‑glycerol phosphate and dexamethasone to differentiate into osteoblasts. Biological in vitro analysis showed the rapid proliferation of the MSCs. Further evaluation of specific osteogenic markers using von Kossa staining and the alkaline phosphate assay demonstrated that the MSCs were stimulated to differentiate into osteoblast‑lineage cells. This mesengenic potential indicated that the bone marrow‑derived cells were multipotent MSCs. The findings of this study show that bone marrow can be a legitimate source of MSCs for the production of osteoblasts for utilization in bone replacement therapy.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow

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