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Article

Downregulation of microRNA-21 enhances radiosensitivity in nasopharyngeal carcinoma

  • Authors:
    • Honghai Zhu
    • Xiaoyuan Zhu
    • Genyang Cheng
    • Minghui Zhou
    • Weihua Lou
  • View Affiliations / Copyright

    Affiliations: Department of Rhinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Otorhinolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 2185-2189
    |
    Published online on: April 2, 2015
       https://doi.org/10.3892/etm.2015.2403
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Abstract

Radioresistance severely restricts the clinical treatment of nasopharyngeal carcinoma (NPC). microRNAs (miRs) have been demonstrated to affect cancer progression and radiosensitivity. Thus, the aim of the present study was to identify miRs associated with radioresistance in NPC. A radioresistant NPC cell line (CNE‑2‑1) was established by continuously exposing CNE‑2 cells to radiation. Subsequently, high‑throughput sequencing technology was used to detect the regulation of miRs in radioresistant CNE‑2‑1 cells, and it was observed that miR-21 was among the three most upregulated miRs in CNE-2-1 cells. Therefore, the expression levels of miR‑21 were quantified using reverse transcription‑quantitative polymerase chain reaction. Finally, the function of miR‑21 was investigated by downregulating the expression in the CNE‑2‑1 cells. The results indicated that the expression of miR‑21 was significantly increased in the CNE‑2‑1 cells, as compared with the CNE‑2 cells. In addition, downregulation of miR‑21 resulted in enhanced radiosensitivity in the CNE‑2‑1 cells, as demonstrated by the inhibition in cell viability of these radioresistant cells. Further analysis indicated that miR‑21 was able to inhibit the proliferation of CNE‑2‑1 cells at the G1 phase of the cell cycle. Therefore, these results indicated that miR‑21 was able to regulate radioresistance in NPC cells; however, further studies are required to confirm this hypothesis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu H, Zhu X, Cheng G, Zhou M and Lou W: Downregulation of microRNA-21 enhances radiosensitivity in nasopharyngeal carcinoma. Exp Ther Med 9: 2185-2189, 2015.
APA
Zhu, H., Zhu, X., Cheng, G., Zhou, M., & Lou, W. (2015). Downregulation of microRNA-21 enhances radiosensitivity in nasopharyngeal carcinoma. Experimental and Therapeutic Medicine, 9, 2185-2189. https://doi.org/10.3892/etm.2015.2403
MLA
Zhu, H., Zhu, X., Cheng, G., Zhou, M., Lou, W."Downregulation of microRNA-21 enhances radiosensitivity in nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 9.6 (2015): 2185-2189.
Chicago
Zhu, H., Zhu, X., Cheng, G., Zhou, M., Lou, W."Downregulation of microRNA-21 enhances radiosensitivity in nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 9, no. 6 (2015): 2185-2189. https://doi.org/10.3892/etm.2015.2403
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu H, Zhu X, Cheng G, Zhou M and Lou W: Downregulation of microRNA-21 enhances radiosensitivity in nasopharyngeal carcinoma. Exp Ther Med 9: 2185-2189, 2015.
APA
Zhu, H., Zhu, X., Cheng, G., Zhou, M., & Lou, W. (2015). Downregulation of microRNA-21 enhances radiosensitivity in nasopharyngeal carcinoma. Experimental and Therapeutic Medicine, 9, 2185-2189. https://doi.org/10.3892/etm.2015.2403
MLA
Zhu, H., Zhu, X., Cheng, G., Zhou, M., Lou, W."Downregulation of microRNA-21 enhances radiosensitivity in nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 9.6 (2015): 2185-2189.
Chicago
Zhu, H., Zhu, X., Cheng, G., Zhou, M., Lou, W."Downregulation of microRNA-21 enhances radiosensitivity in nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 9, no. 6 (2015): 2185-2189. https://doi.org/10.3892/etm.2015.2403
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