Molecular cloning and expression of the calmodulin gene from guinea pig hearts

Feng,Rui; Liu,Yan; Sun,Xuefei; Wang,Yan; Hu,Huiyuan; Guo,Feng; Zhao,Jinsheng; Hao,Liying

doi:10.3892/etm.2015.2411

Molecular cloning and expression of the calmodulin gene from guinea pig hearts

Authors:
- Rui Feng
- Yan Liu
- Xuefei Sun
- Yan Wang
- Huiyuan Hu
- Feng Guo
- Jinsheng Zhao
- Liying Hao
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Affiliations: Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, Liaoning 110001, P.R. China
Published online on: April 7, 2015 https://doi.org/10.3892/etm.2015.2411
Pages: 2311-2318

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Abstract

The aim of the present study was to isolate and characterize a complementary DNA (cDNA) clone encoding the calmodulin (CaM; GenBank accession no. FJ012165) gene from guinea pig hearts. The CaM gene was amplified from cDNA collected from guinea pig hearts and inserted into a pGEM®‑T Easy vector. Subsequently, CaM nucleotide and protein sequence similarity analysis was conducted between guinea pigs and other species. In addition, reverse transcription‑polymerase chain reaction (RT‑PCR) was performed to investigate the CaM 3 expression patterns in different guinea pig tissues. Sequence analysis revealed that the CaM gene isolated from the guinea pig heart had ~90% sequence identity with the CaM 3 genes in humans, mice and rats. Furthermore, the deduced peptide sequences of CaM 3 in the guinea pig showed 100% homology to the CaM proteins from other species. In addition, the RT‑PCR results indicated that CaM 3 was widely and differentially expressed in guinea pigs. In conclusion, the current study provided valuable information with regard to the cloning and expression of CaM 3 in guinea pig hearts. These findings may be helpful for understanding the function of CaM3 and the possible role of CaM3 in cardiovascular diseases.

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