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Effect of erythropoietin on the expression of dynamin-related protein-1 in rat renal interstitial fibrosis

  • Authors:
    • Xian‑Feng Zhao
    • Yan‑Hong Liu
    • Zi‑Ming Han
    • Yu Xu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Medicine, Henan Provincial People's Hospital, Zhengzhou, Henan 450000, P.R. China, Department of Neonatology, Zhengzhou People's Hospital, Zhengzhou, Henan 450000, P.R. China, Department of Pediatrics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2065-2071
    |
    Published online on: April 9, 2015
       https://doi.org/10.3892/etm.2015.2419
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Abstract

This study aimed to observe the expression of dynamin‑related protein‑1 (Drp‑1) in the renal interstitium in a rat model of renal interstitial fibrosis induced by unilateral ureteral obstruction (UUO). In addition, the renoprotective effect of erythropoietin in this model was investigated. A total of 81 rats were randomly assigned to sham surgery, UUO model and treatment groups. Following surgery, the rats in the treatment group were subcutaneously administered erythropoietin at a dose of 3,000 IU/kg once a week until the time of sacrifice. Rats in the sham surgery and UUO model groups were administered an identical volume of normal saline. In each group, nine rats were chosen randomly for sacrifice on days 7, 14 and 21 after surgery for histological examination of renal tissue. Renal tissue specimens were examined by hematoxylin and eosin and Masson's trichrome staining. Immunohistochemical analysis was performed to determine the expression of Drp‑1 in the renal interstitium. Renal function damage, as evaluated by the measurement of serum creatinine (Cr) and blood urea nitrogen (BUN) levels, was less severe in the treatment group compared with that in the model group at day 21 (P<0.01). Compared with the UUO model group, the renal interstitial injury score and fibrotic area of the treatment group were decreased markedly at the three time points (P<0.05). The expression level of Drp‑1 in the treatment group was decreased markedly at the three time points compared with that in the model group (P<0.05). In conclusion, the expression of Drp‑1 is increased in rat renal interstitial fibrosis, and erythropoietin may alleviate the degree of renal interstitial fibrosis by downregulating the expression of Drp‑1.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao XF, Liu YH, Han ZM and Xu Y: Effect of erythropoietin on the expression of dynamin-related protein-1 in rat renal interstitial fibrosis. Exp Ther Med 9: 2065-2071, 2015.
APA
Zhao, X., Liu, Y., Han, Z., & Xu, Y. (2015). Effect of erythropoietin on the expression of dynamin-related protein-1 in rat renal interstitial fibrosis. Experimental and Therapeutic Medicine, 9, 2065-2071. https://doi.org/10.3892/etm.2015.2419
MLA
Zhao, X., Liu, Y., Han, Z., Xu, Y."Effect of erythropoietin on the expression of dynamin-related protein-1 in rat renal interstitial fibrosis". Experimental and Therapeutic Medicine 9.6 (2015): 2065-2071.
Chicago
Zhao, X., Liu, Y., Han, Z., Xu, Y."Effect of erythropoietin on the expression of dynamin-related protein-1 in rat renal interstitial fibrosis". Experimental and Therapeutic Medicine 9, no. 6 (2015): 2065-2071. https://doi.org/10.3892/etm.2015.2419
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao XF, Liu YH, Han ZM and Xu Y: Effect of erythropoietin on the expression of dynamin-related protein-1 in rat renal interstitial fibrosis. Exp Ther Med 9: 2065-2071, 2015.
APA
Zhao, X., Liu, Y., Han, Z., & Xu, Y. (2015). Effect of erythropoietin on the expression of dynamin-related protein-1 in rat renal interstitial fibrosis. Experimental and Therapeutic Medicine, 9, 2065-2071. https://doi.org/10.3892/etm.2015.2419
MLA
Zhao, X., Liu, Y., Han, Z., Xu, Y."Effect of erythropoietin on the expression of dynamin-related protein-1 in rat renal interstitial fibrosis". Experimental and Therapeutic Medicine 9.6 (2015): 2065-2071.
Chicago
Zhao, X., Liu, Y., Han, Z., Xu, Y."Effect of erythropoietin on the expression of dynamin-related protein-1 in rat renal interstitial fibrosis". Experimental and Therapeutic Medicine 9, no. 6 (2015): 2065-2071. https://doi.org/10.3892/etm.2015.2419
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