Mitomycin-C suppresses mucus secretion in an ileal neobladder rat model

Fan,Weiwei; Yu,Yang; Shu,Junjie; Ming,Hao; Li,Weiping; Fan,Zhilu

doi:10.3892/etm.2015.2529

August-2015 Volume 10 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

August-2015 Volume 10 Issue 2

Full Size Image

Article

Mitomycin-C suppresses mucus secretion in an ileal neobladder rat model

Authors:
- Weiwei Fan
- Yang Yu
- Junjie Shu
- Hao Ming
- Weiping Li
- Zhilu Fan
View Affiliations / Copyright

Affiliations: Dalian Medical University, Dalian, Liaoning 116085, P.R. China, Department of Urinary Surgery, Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China
Pages: 598-602
|
Published online on: May 28, 2015

https://doi.org/10.3892/etm.2015.2529
Expand metrics +

Abstract

The aim of the present study was to investigate the mucus secretion status of mature goblet cells following the application of mitomycin‑C (MMC) in ileal neobladder rat models. Bladder substitution models were established in Sprague Dawley rats, which had been divided into five groups, namely the control (sham), normal saline (NS), high‑dose MMC (HMMC), low‑dose MMC (LMMC) and dehydrated alcohol (DA) groups. To evaluate the total protein concentration and level of sialic acid following the therapy, urine from the rats in each group was collected on days 8, 11 and 14. In addition, to observe the variances between mucus secretion and the ileum goblet cells, immunohistochemistry and hematoxylin and eosin staining were conducted in the different groups on day 17. The results indicated that the ileal neobladder mucosas in the MMC groups were clearly undamaged, as compared with the DA group. Furthermore, the MMC and DA groups were shown to inhibit the proliferation of goblet cells. The concentration of protein and sialic acid in the LMMC group was found to be lower compared with the NS group, while the concentration in the HMMC group was considerably lower. In conclusion, HMMC was demonstrated to evidently reduce the mucin and sialic acid concentration in the urine, without visible damage to the ileal neobladder mucus membrane. Therefore, MMC may provide a novel therapeutic approach for the treatment of certain bladder conditions.

View Figures

View References

1	Kashif KM and Holmes SA: The use of small intestine in bladder reconstruction. Int Urogynecol J Pelvic Floor Dysfunct. 9:275–280. 1998. View Article : Google Scholar : PubMed/NCBI
2	Sun X, Song M, Bai R, Cheng S, Xing Y, Yuan H, Wang P and Zhou L: Ileal interposition surgery-induced improvement of hyperglycemia and insulin resistance in Goto-Kakizaki rats by upregulation of TCF7L2 expression. Exp Ther Med. 5:1511–1515. 2013.PubMed/NCBI
3	Hautmann RE, de Petriconi RC and Volkmer BG: Lessons learned from 1,000 neobladders: The 90-day complication rate. J Urol. 184:990–994. 2010. View Article : Google Scholar : PubMed/NCBI
4	Yu Yang: The experimental studies of anti-tumor agents to intestinal mucus secretion in vitro. (unpublished PhD thesis)Dalian Medical University2006, (In Chinese).
5	Demirbilek S, Uğuralp S, Gürbüz N, Sezgin N and Kirimlioĝlu H: The use of silver nitrate for chemical de-epithelialization and urothelialization of intestine in a rabbit model of augmentation cystoplasty. Urol Res. 31:236–241. 2003. View Article : Google Scholar : PubMed/NCBI
6	Leknes IL: Histochemical study on the intestine goblet cells in cichlid and poecilid species (Teleostei). Tissue Cell. 42:61–64. 2010. View Article : Google Scholar : PubMed/NCBI
7	Khorrami MH, Salehi P, Nouri-Mahdavi K, Ghalamkari A and Tadayyon F: Dramatic effect of a somatostatin analogue in decreasing mucus production by the intestinal segment after enterocystoplasty. J Urol. 180:2501–2503. 2008. View Article : Google Scholar : PubMed/NCBI
8	Zhang T and Zhou X: Clinical application of expectorant therapy in chronic inflammatory airway diseases (Review). Exp Ther Med. 7:763–767. 2014.PubMed/NCBI
9	Falke G, Caffaratti J and Atala A: Tissue engineering of the bladder. World J Urol. 18:36–43. 2000. View Article : Google Scholar : PubMed/NCBI
10	Loeffler M, Birke A, Winton D and Potten C: Somatic mutation, monoclonality and stochastic models of stem cell organization in the intestinal crypt. J Theor Biol. 160:471–491. 1993. View Article : Google Scholar : PubMed/NCBI
11	Shen G-J, Datta AK, Izumi M, Koeller KM and Wong C-H: Expression of α2, 8/2, 9-polysialyltransferase from Escherichia coli K92. Characterization of the enzyme and its reaction products. J Boil Chen. 274:35139–35146. 1999. View Article : Google Scholar
12	Levine MJ, Aguirre A, Hatton MN and Tabak LA: Artifical salivas: Present and future. J Dent Res. 66:693–698. 1987.PubMed/NCBI
13	Matsuo K, Ota H, Akamatsu T, Sugiyama A and Katsuyama T: Histochemistry of the surface mucous gel layer of the human colon. Gut. 40:782–789. 1997. View Article : Google Scholar : PubMed/NCBI
14	Baylin SB: Reversal of gene silencing as a therapeutic target for cancer-roles for DNA methylation and its interdigitation with chromatin. Novartis Found Symp. 259:226–233. 2004.PubMed/NCBI
15	Ozdemir F, Altinisik J, Karateke A, Coksuer H and Buyru N: Methylation of tumor suppressor genes in ovarian cancer. Exp Ther Med. 4:1092–1096. 2012.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Mitomycin-C suppresses mucus secretion in an ileal neobladder rat model

This article is mentioned in:

Abstract

Figure 1

Figure 2

Related Articles