Expression of vascular cell adhesion molecule-1 in the aortic tissues of atherosclerotic patients and the associated clinical implications

Mu,Wei; Chen,Mingyou; Gong,Zushun; Zheng,Fei; Xing,Qichong

doi:10.3892/etm.2015.2540

August-2015 Volume 10 Issue 2

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August-2015 Volume 10 Issue 2

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Article Open Access

Expression of vascular cell adhesion molecule-1 in the aortic tissues of atherosclerotic patients and the associated clinical implications

Authors:
- Wei Mu
- Mingyou Chen
- Zushun Gong
- Fei Zheng
- Qichong Xing
View Affiliations / Copyright

Affiliations: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, Ji'nan, Shandong 250014, P.R. China

Copyright: © Mu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 423-428
|
Published online on: June 3, 2015

https://doi.org/10.3892/etm.2015.2540
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Abstract

The aim of this study was to investigate the expression level of vascular cell adhesion molecule‑1 (VCAM‑1) in the aortic tissues of atherosclerotic patients and to explore the associated clinical implications. Full‑thickness aortic wall tissue samples were collected from atherosclerotic patients. Biochemical analysis was used for the detection of the serum levels of triglycerides (TG), total cholesterol (TC), low‑density lipoprotein cholesterol (LDL‑C), high‑density lipoprotein cholesterol (HDL‑C), lipoprotein (a) [Lp (a)], apolipoprotein (Apo) AI and Apo‑B. Coronary angiography and SYNTAX scoring were used to determine the extent and severity of the disease. Immunohistochemistry was employed for the detection of the VCAM‑1 protein expression levels in the arterial tissues. Significant differences were observed in the blood lipid levels between atherosclerotic patients and control subjects. Immunohistochemistry indicated that the aortic VCAM‑1 expression level in atherosclerotic patients was 0.23±0.06 optical density (OD) units, which was significantly higher than that in the control subjects (0.08±0.03 OD units). In the atherosclerotic patients, the aortic VCAM‑1 expression was positively correlated with the serum levels of TG (r=0.347), TC (r=0.469), LDL‑C (r=0.463), Lp (a) (r=0.507) and Apo‑B (r=0.384), while VCAM‑1 and HDL‑C were negatively correlated (r=‑0.319). Furthermore, a higher SYNTAX score was accompanied by a higher VCAM‑1 expression level (r=0.532), and an elevated aortic VCAM‑1 expression was associated with certain cardiovascular risk factors. In conclusion, aortic VCAM‑1 expression is associated with the severity of atherosclerosis and cardiovascular risk factors, indicating that VCAM‑1 plays a role in the pathogenesis of atherosclerosis.

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1	Ross R: Atherosclerosis - an inflammatory disease. N Engl J Med. 340:115–126. 1999. View Article : Google Scholar : PubMed/NCBI
2	Wolf D, Stachon P, Bode C and Zirlik A: Inflammatory mechanisms in atherosclerosis. Hamostaseologie. 34:63–71. 2014. View Article : Google Scholar : PubMed/NCBI
3	Nakashima Y, Raines EW, Plump AS, Breslow JL and Ross R: Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the ApoE-deficient mouse. Arterioscler Thromb Vasc Biol. 18:842–851. 1998. View Article : Google Scholar : PubMed/NCBI
4	Sitia S, Tomasoni L, Atzeni F, et al: From endothelial dysfunction to atherosclerosis. Autoimmun Rev. 9:830–834. 2010. View Article : Google Scholar : PubMed/NCBI
5	Liu B, Wang J, Cheng L and Liang J: Role of JNK and NF-κB pathways in Porphyromonas gingivalis LPS-induced vascular cell adhesion molecule-1 expression in human aortic endothelial cells. Mol Med Rep. 8:1594–1600. 2013.PubMed/NCBI
6	De Caterina R, Basta G, Lazzerini G, et al: Soluble vascular cell adhesion molecule-1 as a biohumoral correlate of atherosclerosis. Arterioscler Thromb Vasc Biol. 17:2646–2654. 1997. View Article : Google Scholar : PubMed/NCBI
7	Zeitler H, Ko Y, Zimmermann C, et al: Elevated serum concentrations of soluble adhesion molecules in coronary artery disease and acute myocardial infarction. Eur J Med Res. 2:389–394. 1997.PubMed/NCBI
8	Lee MS, Applegate B, Rao SV, Kirtane AJ, Seto A and Stone GW: Minimizing femoral artery access complications during percutaneous coronary intervention: A comprehensive review. Catheter Cardiovasc Interv. 84:62–69. 2014. View Article : Google Scholar : PubMed/NCBI
9	Sianos G, Morel MA, Kappetein AP, et al: The SYNTAX Score: An angiographic tool grading the complexity of coronary artery disease. EuroIntervention. 1:219–227. 2005.PubMed/NCBI
10	Tan HW, Xing SS, Bi XP, et al: Felodipine attenuates vascular inflammation in a fructose-induced rat model of metabolic syndrome via the inhibition of NF-kappaB activation. Acta Pharmacol Sin. 29:1051–1059. 2008. View Article : Google Scholar : PubMed/NCBI
11	DeVerse JS, Sandhu AS, Mendoza N, Edwards CM, Sun C, Simon SI and Passerini AG: Shear stress modulates VCAM-1 expression in response to TNF-α and dietary lipids via interferon regulatory factor-1 in cultured endothelium. Am J Physiol Heart Circ Physiol. 305:H1149–H1157. 2013. View Article : Google Scholar : PubMed/NCBI
12	Fotis L, Agrogiannis G, Vlachos IS, et al: Intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 at the early stages of atherosclerosis in a rat model. In Vivo. 26:243–250. 2012.PubMed/NCBI
13	Cook-Mills JM: VCAM-1 signals during lymphocyte migration: Role of reactive oxygen species. Mol Immunol. 39:499–508. 2002. View Article : Google Scholar : PubMed/NCBI
14	Petersen EJ, Miyoshi T, Yuan Z, Hirohata S, Li JZ, Shi W and Angle JF: siRNA silencing reveals role of vascular cell adhesion molecule-1 in vascular smooth muscle cell migration. Atherosclerosis. 198:301–306. 2008. View Article : Google Scholar : PubMed/NCBI
15	Cybulsky MI and Gimbrone MA Jr: Endothelial expression of a mononuclear leukocyte adhesion molecule during atherogenesis. Science. 251:788–791. 1991. View Article : Google Scholar : PubMed/NCBI
16	Lu HH, Sheng ZQ, Wang Y and Zhang L: Levels of soluble adhesion molecules in patients with various clinical presentations of coronary atherosclerosis. Chin Med J (Engl). 123:3123–3126. 2010.PubMed/NCBI
17	Karasek D, Vaverkova H, Frysak Z, Halenka M, Jackuliakova D, Novotny D and Lukes J: Soluble intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 in asymptomatic dyslipidemic subjects. Int Angiol. 30:441–450. 2011.PubMed/NCBI
18	Nakai K, Itoh C, Kawazoe K, et al: Concentration of soluble vascular cell adhesion molecule-1 (VCAM-1) correlated with expression of VCAM-1 mRNA in the human atherosclerotic aorta. Coron Artery Dis. 6:497–502. 1995.PubMed/NCBI
19	Leca G, Mansur SE and Bensussan A: Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform. J Immunol. 154:1069–1077. 1995.PubMed/NCBI
20	Hackman A, Abe Y, Insull W Jr, et al: Levels of soluble cell adhesion molecules in patients with dyslipidemia. Circulation. 93:1334–1338. 1996. View Article : Google Scholar : PubMed/NCBI
21	Saidi H, Vakilian M, Noori GH, Ghafouri HB and Abazarian N: Alterations in circulating adhesion molecules in acute myocardial infarction before and after thrombolysis with streptokinase. J Cardiovasc Thorac Res. 5:139–141. 2013.PubMed/NCBI
22	Steinberg D and Witztum JL: Oxidized low-density lipoprotein and atherosclerosis. Arterioscler Thromb Vasc Biol. 30:2311–2316. 2010. View Article : Google Scholar : PubMed/NCBI
23	Ross R and Harker L: Hyperlipidemia and atherosclerosis. Science. 193:1094–1100. 1976. View Article : Google Scholar : PubMed/NCBI
24	Feig JE, Rong JX, Shamir R, et al: HDL promotes rapid atherosclerosis regression in mice and alters inflammatory properties of plaque monocyte-derived cells. Proc Natl Acad Sci USA. 108:7166–7171. 2011. View Article : Google Scholar : PubMed/NCBI
25	Ashfaq F, Goel PK, Moorthy N, Sethi R, Khan MI and Idris MZ: Lipoprotein(a) and SYNTAX score association with severity of coronary artery atherosclerosis in North India. Sultan Qaboos Univ Med J. 12:465–72. 2012. View Article : Google Scholar : PubMed/NCBI
26	Serruys PW, Morice MC, Kappetein AP, et al: SYNTAX Investigators: Percutaneous coronary intervention versus coronary-artery bypass grafting for severe coronary artery disease. N Engl J Med. 360:961–972. 2009. View Article : Google Scholar : PubMed/NCBI
27	Wykrzykowska JJ, Garg S, Girasis C, et al: Value of the SYNTAX score for risk assessment in the all-comers population of the randomized multicenter LEADERS (Limus Eluted from A Durable versus ERodable Stent coating) trial. J Am Coll Cardiol. 56:272–277. 2010. View Article : Google Scholar : PubMed/NCBI
28	Chakravarty T, Buch MH, Naik H, et al: Predictive accuracy of SYNTAX score for predicting long-term outcomes of unprotected left main coronary artery revascularization. Am J Cardiol. 107:360–366. 2011. View Article : Google Scholar : PubMed/NCBI
29	Edirisinghe I and Rahman I: Cigarette smoke-mediated oxidative stress, shear stress, and endothelial dysfunction: Role of VEGFR2. Ann NY Acad Sci. 1203:66–72. 2010. View Article : Google Scholar : PubMed/NCBI
30	Turnbull F, Kengne AP and MacMahon S: Blood pressure and cardiovascular disease: Tracing the steps from Framingham. Prog Cardiovasc Dis. 53:39–44. 2010. View Article : Google Scholar : PubMed/NCBI
31	Opie LH: Acute myocardial infarction and diabetes. Lancet. 370:634–635. 2007. View Article : Google Scholar : PubMed/NCBI
32	Park JG, Ryu SY, Jung IH, et al: Evaluation of VCAM-1 antibodies as therapeutic agent for atherosclerosis in apolipoprotein E-deficient mice. Atherosclerosis. 226:356–63. 2013. View Article : Google Scholar : PubMed/NCBI
33	Zhang W, Xing SS, Sun XL and Xing QC: Overexpression of activated nuclear factor-kappa B in aorta of patients with coronary atherosclerosis. Clin Cardiol. 32:E42–E47. 2009. View Article : Google Scholar : PubMed/NCBI
34	Gong YL, Xing SS, Zhang W and Xing QC: Relationship between Toll-like receptor 4 protein and atherosclerosis. Shan Dong Da Xue Xue (Yi Xue Ban). 48:127–130. 2010.(In Chinese).
35	Xing SS, Gong ZS, Mu W, Wang D, Gong YL and Xing QC: Expression of human living aortic high mobility group protein box1 and its relationship with the pathogenesis of atherosclerosis. Shan Dong Da Xue Xue (Yi Xue Ban). 51:62–65. 2013.(In Chinese).