Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

  • Authors:
    • Wenfeng Huang
    • Kun Zou
  • View Affiliations / Copyright

    Affiliations: Hubei Key Laboratory of Natural Products Research and Development, Medical College, China Three Gorges University, Yichang, Hubei 443002, P.R. China
  • Pages: 625-628
    |
    Published online on: June 3, 2015
       https://doi.org/10.3892/etm.2015.2544
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The steroidal saponin TTB2 can be isolated from the n-BuOH extracts of Trillium tschonoskii Maxim. The aim of the present study was to observe whether this saponin exerted any cytotoxic effects on malignant sarcoma cells, and to further investigate the possible underlying molecular mechanisms. The cell viability, cell cycle arrest and phosphorylation of certain important signal molecules in the sarcoma cell line were investigated. It was found that TTB2 inhibited the growth of the Ewing sarcoma cell line and arrested cells in the G2/M and S phases of the cell cycle in a dose‑ and time‑dependent manner. Furthermore, the phosphorylation of extracellular signal‑regulated kinase was inhibited by TTB2. In conclusion, the results showed that the saponin TTB2 isolated from T. tschonoskii Maxim exerts anticancer effects and may be a potential candidate for the development of anticancer drugs for use in the treatment of cancer.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Mansilla S, Llovera L and Portugal J: Chemotherapeutic targeting of cell death pathways. Anticancer Agents Med Chem. 12:226–238. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Rasul A, Song R, Wei W, et al: Tubeimoside-1 inhibits growth via the induction of cell cycle arrest and apoptosis in human melanoma A375 cells. Bangladesh J Pharmacol. 7:150–156. 2012. View Article : Google Scholar

3 

Fu L: China Plant Red Data Book: Rare and Endangered Plants. 1. Science Press; Beijing: 1992

4 

Li Q, Xiao M, Guo L, et al: Genetic diversity and genetic structure of an endangered species, Trillium tschonoskii. Biochem Genet. 43:445–458. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Hayes PY, Lehmann R, Penman K, Kitching W and De Voss JJ: Steroidal saponins from the roots of Trillium erectum (Beth root). Phytochemistry. 70:105–113. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Yokosuka A and Mimaki Y: Steroidal glycosides from the underground parts of Trillium erectum and their cytotoxic activity. Phytochemistry. 69:2724–2730. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Ono M, Sugita F, Shigematsu S, et al: Three new steroid glycosides from the underground parts of Trillium kamtschaticum. Chem Pharm Bull (Tokyo). 55:1093–1096. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Nohara T, Miyahara K and Kawasaki T: Steroid saponins and sapogenins of underground parts of Trillium kamtschaticum Pall. II. Pennogenin- and kryptogenin 3-O-glycosides and related compounds. Chem Pharm Bull (Tokyo). 23:872–885. 1975. View Article : Google Scholar

9 

Nohara T, Kumamoto F, Miyahara K and Kawasaki T: Steroid saponins of aerial parts of Paris tetraphylla A. Gray and of underground parts of Trillium tschonoskii Maxim. Chem Pharm Bull (Tokyo). 23:1158–1160. 1975. View Article : Google Scholar

10 

Osbourn A, Goss RJ and Field RA: The saponins: Polar isoprenoids with important and diverse biological activities. Nat Prod Rep. 28:1261–1268. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Fuchs H, Bachran D, Panjideh H, Schellmann N, Weng A, Melzig MF, Sutherland M and Bachran C: Saponins as tool for improved targeted tumor therapies. Curr Drug Targets. 10:140–151. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Yang SL, Liu XK, Wu H, Wang HB and Qing C: Steroidal saponins and cytoxicity of the wild edible vegetable-Smilacina atropurpurea. Steroids. 74:7–12. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Tyagi A, Bhatia N, Condon MS, Bosland MC, Agarwal C and Agarwal R: Antiproliferative and apoptotic effects of silibinin in rat prostate cancer cells. Prostate. 53:211–217. 2002. View Article : Google Scholar : PubMed/NCBI

14 

De Luca A, Maiello MR, D'Alessio A, Pergameno M and Normanno N: The RAS/RAF/MEK/ERK and the PI3K/AKT signalling pathways: role in cancer pathogenesis and implications for therapeutic approaches. Expert Opin Ther Targets. 16 Suppl 2:S17–27. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Güçlü-Ustündaǧ O and Mazza G: Saponins: properties, applications and processing. Crit Rev Food Sci Nutr. 47:231–258. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Chen CX, Zhou J, Zhang YT and Zhao YY: Steroid saponins of aerial parts of Paris polyphylla var. yunnanensis. Acta Bot Yunnan. 12:323–329. 1990.(In Chinese).

17 

Matsuda H, Pongpiriyadacha Y, Morikawa T, Kishi A, Kataoka S and Yoshikawa M: Protective effects of steroid saponins from Paris polyphylla var. yunnanensis on ethanol- or indomethacin-induced gastric mucosal lesions in rats: structural requirement for activity and mode of action. Bioorg Med Chem Lett. 13:1101–1106. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Kougan GB, Miyamoto T, Tanaka C, et al: Steroidal saponins from two species of Dracaena. J Nat Prod. 73:1266–1270. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Huang Y, Cui LJ, Wang Q and Ye WC: Separation and identification of active constituents of Paris vietnamensis. Yao Xue Xue Bao. 41:361–364. 2006.(In Chinese). PubMed/NCBI

20 

Zhou BB and Elledge SJ: The DNA damage response: putting checkpoints in perspective. Nature. 408:433–439. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Moelling K, Schad K, Bosse M, Zimmermann S and Schweneker M: Regulation of Raf-Akt Cross-talk. J Biol Chem. 277:31099–31106. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Roberts PJ and Der CJ: Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer. Oncogene. 26:3291–3310. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Huang W and Zou K: Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells. Exp Ther Med 10: 625-628, 2015.
APA
Huang, W., & Zou, K. (2015). Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells. Experimental and Therapeutic Medicine, 10, 625-628. https://doi.org/10.3892/etm.2015.2544
MLA
Huang, W., Zou, K."Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells". Experimental and Therapeutic Medicine 10.2 (2015): 625-628.
Chicago
Huang, W., Zou, K."Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells". Experimental and Therapeutic Medicine 10, no. 2 (2015): 625-628. https://doi.org/10.3892/etm.2015.2544
Copy and paste a formatted citation
x
Spandidos Publications style
Huang W and Zou K: Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells. Exp Ther Med 10: 625-628, 2015.
APA
Huang, W., & Zou, K. (2015). Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells. Experimental and Therapeutic Medicine, 10, 625-628. https://doi.org/10.3892/etm.2015.2544
MLA
Huang, W., Zou, K."Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells". Experimental and Therapeutic Medicine 10.2 (2015): 625-628.
Chicago
Huang, W., Zou, K."Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells". Experimental and Therapeutic Medicine 10, no. 2 (2015): 625-628. https://doi.org/10.3892/etm.2015.2544
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team