Effect of Wujia Shenghua capsule on uterine bleeding following medically-induced incomplete abortion in rats during early pregnancy

Liu,Haiyang; Ma,Wei; Liu,Qi; Wang,Fashan; Cao,Ling; Li,Tienan; Zhang,Ning; Geng,Fang

doi:10.3892/etm.2015.2548

Effect of Wujia Shenghua capsule on uterine bleeding following medically-induced incomplete abortion in rats during early pregnancy

Authors:
- Haiyang Liu
- Wei Ma
- Qi Liu
- Fashan Wang
- Ling Cao
- Tienan Li
- Ning Zhang
- Fang Geng
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Affiliations: College of Jiamusi, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China, Duoduo Pharmaceutical Co. Ltd., Jiamusi, Heilongjiang 154002, P.R. China, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, Heilongjiang 150025, P.R. China
Published online on: June 5, 2015 https://doi.org/10.3892/etm.2015.2548
Pages: 635-640

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Abstract

The Wujia Shenghua capsule (WSC) is derived from Sheng-Hua-Tang, a well-known traditional Chinese medicine compound prescription that has been widely applied during the postpartum period in Chinese communities for a number of years. The aim of the present study was to investigate the effect of WSC on uterine bleeding following medically‑induced incomplete abortion in rats during early pregnancy. Incomplete abortions were induced in Wistar rats during early pregnancy using mifepristone combined with misoprostol. The effects of WSC treatment were investigated in terms of the duration and volume of uterine bleeding, the uterine index and shape, and various hemodynamic indexes. In addition, blood samples were collected to measure the levels of estradiol (E2), fibronectin (FN) and laminin (LM) via a radioimmunoassay or enzyme‑linked immunosorbent assay, while the expression levels of FN, estrogen receptor (ER) and progesterone receptor (PR) in the uterine tissues were determined by immunohistochemistry. The in vivo results demonstrated that WSC treatment markedly shortened the duration and reduced the volume of uterine bleeding when compared with the model group. Furthermore, WSC treatment significantly elevated the E2 level in the serum and the expression of the ER and PR in the uterine tissues, while notably decreasing the FN and LM levels in the serum and uterine tissues. In addition, the hemodynamic indexes were shown to improve with WSC treatment. These results demonstrated that WSC exerted an inhibitory effect on the bleeding caused by medical abortion, possibly through modulating the E2, ER, PR, FN and LM levels.

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1	Bartz D and Goldberg A: Medication abortion. Clin Obstet Gynecol. 52:140–150. 2009. View Article : Google Scholar : PubMed/NCBI
2	Doraiswami S, Johnson T, Rao S, Rajkumar A, Vijayaraghavan J and Panicker VK: Study of endometrial pathology in abnormal uterine bleeding. J Obstet Gynaecol India. 61:426–430. 2011. View Article : Google Scholar : PubMed/NCBI
3	Spitz IM: Mifepristone: where do we come from and where are we going? Clinical development over a quarter of a century. Contraception. 82:442–452. 2010. View Article : Google Scholar : PubMed/NCBI
4	Chuang CH, Chang PJ, Hsieh WS, Tsai YJ, Lin SJ and Chen PC: Chinese herbal medicine use in Taiwan during pregnancy and the postpartum period: a population-based cohort study. Int J Nurs Stud. 46:787–795. 2009. View Article : Google Scholar : PubMed/NCBI
5	Liao HL, Ma TC, Chiu YL, Chen JT and Chang YS: Factors influencing the purchasing behavior of TCM outpatients in Taiwan. J Altern Complement Med. 14:741–748. 2008. View Article : Google Scholar : PubMed/NCBI
6	Chang PJ, Tseng YC, Chuang CH, et al: Use of Sheng-Hua-Tang and health-related quality of life in postpartum women: a population-based cohort study in Taiwan. Int J Nurs Stud. 47:13–19. 2010. View Article : Google Scholar : PubMed/NCBI
7	Ho M, Li TC and Su SY: The association between traditional Chinese dietary and herbal therapies and uterine involution in postpartum women. Evid Based Complement Alternat Med. 2011:9182912011. View Article : Google Scholar : PubMed/NCBI
8	Geng F, Wang F, Zou T, et al: Rapid identification of the multiple absorbed bioactive components and metabolites in rat serum after oral administration of Wu-Jia Sheng-Hua capsule by UPLC-ESI-MS. J Anal Methods Chem. 2013:3189612013. View Article : Google Scholar : PubMed/NCBI
9	IRAC (Interagency Research Animal Committee), . U.S. Government Principles for the Utilization and Care of Vertebrate Animals Used in Testing, Research and TrainingFederal Register. Office of Technology Policy; Washington: pp. 116–118. 1985
10	Li X, Yuan FL, Zhao YQ, Lu WG, Li CW and He CH: Effect of leonurine hydrochloride on endothelin and the endothelin receptor-mediated signal pathway in medically-induced incomplete abortion in rats. Eur J Obstet Gynecol Reprod Biol. 169:299–303. 2013. View Article : Google Scholar : PubMed/NCBI
11	Cheng L: Medical abortion in early pregnancy: experience in China. Contraception. 74:61–65. 2006. View Article : Google Scholar : PubMed/NCBI
12	Li L, Zhou Z and Huang L: Abnormal expression of MMP-9 and imbalance of MMP-9/TIMP-1 is associated with prolonged uterine bleeding after a medical abortion with mifepristone and misoprostol. Acta Obstet Gynecol Scand. 88:673–679. 2009. View Article : Google Scholar : PubMed/NCBI
13	Li X, Yuan FL, Zhao YQ, et al: Effects of leonurine hydrochloride on medically induced incomplete abortion in early pregnancy rats. Eur J Obstet Gynecol Reprod Biol. 159:375–380. 2011. View Article : Google Scholar : PubMed/NCBI
14	Livingstone M and Fraser IS: Mechanisms of abnormal uterine bleeding. Hum Reprod Update. 8:60–67. 2002. View Article : Google Scholar : PubMed/NCBI
15	Lockwood CJ: Mechanisms of normal and abnormal endometrial bleeding. Menopause. 18:408–411. 2011. View Article : Google Scholar : PubMed/NCBI
16	Smith SK: Regulation of angiogenesis in the endometrium. Trends Endocrinol Metab. 12:147–151. 2001. View Article : Google Scholar : PubMed/NCBI
17	Vadlamudi RK, Balasenthil S, Broaddus RR, Gustafsson JA and Kumar R: Deregulation of estrogen receptor coactivator proline-, glutamic acid- and leucine-rich protein-1/modulator of nongenomic activity of estrogen receptor in human endometrial tumors. J Clin Endocrinol Metab. 89:6130–6138. 2004. View Article : Google Scholar : PubMed/NCBI
18	Im A and Appleman LJ: Mifepristone: pharmacology and clinical impact in reproductive medicine, endocrinology and oncology. Expert Opin Pharmacother. 11:481–488. 2010. View Article : Google Scholar : PubMed/NCBI
19	Chwalisz K, Perez MC, Demanno D, Winkel C, Schubert G and Elger W: Selective progesterone receptor modulator development and use in the treatment of leiomyomata and endometriosis. Endocr Rev. 26:423–438. 2005. View Article : Google Scholar : PubMed/NCBI
20	Clark TJ: Modern management of abnormal uterine bleeding. Obstetrician and Gynaecologist. 11:792009. View Article : Google Scholar
21	Fraser IS, Hickey M and Song JY: A comparison of mechanisms underlying disturbances of bleeding caused by spontaneous dysfunctional uterine bleeding or hormonal contraception. Hum Reprod. 11 (Suppl 2):165–178. 1996. View Article : Google Scholar : PubMed/NCBI
22	Williams AR, Critchley HO, Osei J, et al: The effects of the selective progesterone receptor modulator asoprisnil on the morphology of uterine tissues after 3 months treatment in patients with symptomatic uterine leiomyomata. Hum Reprod. 22:1696–1704. 2007. View Article : Google Scholar : PubMed/NCBI
23	Evans J, Kaitu'u-Lino T and Salamonsen LA: Extracellular matrix dynamics in scar-free endometrial repair: perspectives from mouse in vivo and human in vitro studies. Biol Reprod. 85:511–523. 2011. View Article : Google Scholar : PubMed/NCBI
24	Frantz C, Stewart KM and Weaver VM: The extracellular matrix at a glance. J Cell Sci. 123:4195–4200. 2010. View Article : Google Scholar : PubMed/NCBI
25	Hickey M and Fraser IS: The structure of endometrial microvessels. Hum Reprod. 15 (Suppl 3):57–66. 2000. View Article : Google Scholar : PubMed/NCBI
26	Lipowsky HH: Microvascular rheology and hemodynamics. Microcirculation. 12:5–15. 2005. View Article : Google Scholar : PubMed/NCBI