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Article

Protective effects of dexmedetomidine on intestinal ischemia‑reperfusion injury

  • Authors:
    • Yong Sun
    • Qiang Gao
    • Nan Wu
    • Sheng‑De Li
    • Jing‑Xin Yao
    • Wen‑Jie Fan
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Qingdao Women and Children's Hospital, Qingdao, Shandong 266034, P.R. China, Department of Anesthesia, Qingdao Women and Children's Hospital, Qingdao, Shandong 266034, P.R. China
  • Pages: 647-652
    |
    Published online on: June 10, 2015
       https://doi.org/10.3892/etm.2015.2561
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Abstract

Dexmedetomidine (DEX) has been hypothesized to possess anti‑oxidative properties that may mitigate the damage caused by ischemia‑reperfusion (IR) injury. The aim of the present study was to examine the effects of DEX on intestinal contractile activity, inflammation and apoptosis following intestinal IR injury. Intestinal IR injury was induced in rats by complete occlusion of the superior mesenteric artery for 60 min, followed by a 60‑min reperfusion period. Rats received an intraperitoneal injection of 25 µg/kg DEX at 30 min prior to the mesenteric IR injury. Following reperfusion, segments of the terminal ileum were rapidly extracted and transferred into an isolated organ bath. The contractile responses to receptor‑mediated acetylcholine (Ach) and non‑receptor‑mediated potassium chloride (KCl) were subsequently examined. Nitric oxide (NO) levels were determined and the expression levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6, Bax and Bcl‑2 were measured using an enzyme‑linked immunosorbent assay. The levels of telomerase and caspase‑3 were determined using reverse transcription‑quantitative polymerase chain reaction. The results indicated that DEX treatment produced a significant reduction in the IR‑induced contractile response to Ach and KCl in the intestinal tissue. Furthermore, DEX appeared to significantly ameliorate intestinal IR injury, in addition to reducing the production of NO. Similar reductions were observed in the intestinal expression levels of TNF‑α and IL‑6. In addition, DEX treatment resulted in a reduction in the expression levels of Bax in the intestinal tissues, while increasing those of Bcl‑2, in addition to significantly increasing the mRNA levels of telomerase and caspase‑3. Therefore, the present study indicated that NO, TNF‑α and IL‑6 may partially contribute to the pathogenesis of intestinal IR injury in addition to the increased expression levels of Bax, Bcl‑2, telomerase and caspase‑3. These findings suggest that DEX possesses beneficial anti‑apoptotic and anti‑inflammatory effects in intestinal tissue following bowel injury.
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1 

Shen J and Fu G, Jiang L, Xu J, Li L and Fu G: Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia-reperfusion. Exp Ther Med. 6:1359–1364. 2013.PubMed/NCBI

2 

Xia G, Martin AE, Michalsky MP and Besner GE: Heparin-binding EGF-like growth factor preserves crypt cell proliferation and decreases bacterial translocation after intestinal ischemia/reperfusion injury. J Pediatr Surg. 37:1081–1087. 2002. View Article : Google Scholar : PubMed/NCBI

3 

El Assal ON and Besner GE: Heparin-binding epidermal growth factor-like growth factor and intestinal ischemia-reperfusion injury. Semin Pediatr Surg. 13:2–10. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Fujise T, Iwakiri R, Wu B, Amemori S, Kakimoto T, Yokoyama F, Sakata Y, Tsunada S and Fujimoto K: Apoptotic pathway in the rat small intestinal mucosa is different between fasting and ischemia-reperfusion. Am J Physiol Gastrointest Liver Physiol. 291:G110–G116. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Taha MO, Miranda-Ferreira R, Fagundes AL, Fagundes DJ, Simões RS, Santos JM, Souza PD, Oliveira-Júnior IS, Marchini A, Gomes IT, et al: Effects of L-nitro-arginine methyl ester, an inhibitor of nitric oxide biosynthesis, on intestinal ischemia/reperfusion injury in rabbits. Transplant Proc. 42:457–460. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Yang S, Chou WP and Pei L: Effects of propofol on renal ischemia/reperfusion injury in rats. Exp Ther Med. 6:1177–1183. 2013.PubMed/NCBI

7 

Petrat F and de Groot H: Protection against severe intestinal ischemia/reperfusion injury in rats by intravenous resveratrol. J Surg Res. 167:e145–e155. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Hanci V, Erol B, Bektaş S, Mungan G, Yurtlu S, Tokgöz H, Can M and Ozkoçak Turan I: Effect of dexmedetomidine on testicular torsion/detorsion damage in rats. Urol Int. 84:105–111. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Lai YC, Tsai PS and Huang CJ: Effects of dexmedetomidine on regulating endotoxin-induced up-regulation of inflammatory molecules in murine macrophages. J Surg Res. 154:212–219. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Lin S, Dai N, Cheng Z, Shao W and Fu Z: Effect of dexmedetomidine-etomidate-fentanyl combined anesthesia on somatosensory- and motor-evoked potentials in patients undergoing spinal surgery. Exp Ther Med. 7:1383–1387. 2014.PubMed/NCBI

11 

Kiliç K, Hanci V, Selek S, Sözmen M, Kiliç N, Citil M, Yurtlu DA and Yurtlu BS: The effects of dexmedetomidine on mesenteric arterial occlusion-associated gut ischemia and reperfusion-induced gut and kidney injury in rabbits. J Surg Res. 178:223–232. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Ozacmak VH, Sayan H, Arslan SO, Altaner S and Aktas RG: Protective effect of melatonin on contractile activity and oxidative injury induced by ischemia and reperfusion of rat ileum. Life Sci. 76:1575–1588. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Gao Y, Deng XG, Sun QN and Zhong ZQ: Ganoderma spore lipid inhibits N-methyl-N-nitrosourea-induced retinal photoreceptor apoptosis in vivo. Exp Eye Res. 90:397–404. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Hassoun HT, Weisbrodt NW, Mercer DW, Kozar RA, Moody FG and Moore FA: Inducible nitric oxide synthase mediates gut ischemia/reperfusion-induced ileus only after severe insults. J Surg Res. 97:150–154. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Potoka DA, Nadler EP, Upperman JS and Ford HR: Role of nitric oxide and peroxynitrite in gut barrier failure. World J Surg. 26:806–811. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Upperman JS, Potoka D, Grishin A, Hackam D, Zamora R and Ford HR: Mechanisms of nitric oxide-mediated intestinal barrier failure in necrotizing enterocolitis. Semin Pediatr Surg. 14:159–166. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Szadujkis-Szadurska K, Grzesk G, Szadujkis-Szadurski L, Gajdus M and Matusiak G: Role of nitric oxide and cGMP in the modulation of vascular contraction induced by angiotensin II and Bay K8644 during ischemia/reperfusion. Exp Ther Med. 5:616–620. 2013.PubMed/NCBI

18 

Lin CY, Tsai PS, Hung YC and Huang CJ: L-type calcium channels are involved in mediating the anti-inflammatory effects of magnesium sulphate. Br J Anaesth. 104:44–51. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Eser O, Fidan H, Sahin O, Cosar M, Yaman M, Mollaoglu H, Songur A and Buyukbas S: The influence of dexmedetomidine on ischemic rat hippocampus. Brain Res. 1218:250–256. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Taniguchi T, Kurita A, Kobayashi K, Yamamoto K and Inaba H: Dose- and time-related effects of dexmedetomidine on mortality and inflammatory responses to endotoxin-induced shock in rats. J Anesth. 22:221–228. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Can M, Gul S, Bektas S, Hanci V and Acikgoz S: Effects of dexmedetomidine or methylprednisolone on inflammatory responses in spinal cord injury. Acta Anaesthesiol Scand. 53:1068–1072. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Memiş D, Hekimoğlu S, Vatan I, Yandim T, Yüksel M and Süt N: Effects of midazolam and dexmedetomidine on inflammatory responses and gastric intramucosal pH to sepsis, in critically ill patients. Br J Anaesth. 98:550–552. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Venn RM, Bryant A, Hall GM and Grounds RM: Effects of dexmedetomidine on adrenocortical function, and cardiovascular, endocrine and inflammatory responses in post-operative patients needing sedation in the intensive care unit. Br J Anaesth. 86:650–656. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Yang Y, Liu JL, Qin C, Li JP, Wang XL, Zhang ZX and Zhang L: Effects of cerebellar fastigial nucleus electrical stimulation on telomerase reverse transcriptase expression and mitochondrial apoptotic pathway in rats with focal cerebral ischemia and reperfusion. Zhonghua Yi Xue Za Zhi. 91:1643–1648. 2011.(In Chinese). PubMed/NCBI

25 

Lau BW, Wong AO, Tsao GS, So KF and Yip HK: Molecular cloning and characterization of the zebra fish (Danio rerio) telomerase catalytic subunit (telomerase reverse transcriptase, TERT). J Mol Neurosci. 34:63–75. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun Y, Gao Q, Wu N, Li SD, Yao JX and Fan WJ: Protective effects of dexmedetomidine on intestinal ischemia‑reperfusion injury. Exp Ther Med 10: 647-652, 2015.
APA
Sun, Y., Gao, Q., Wu, N., Li, S., Yao, J., & Fan, W. (2015). Protective effects of dexmedetomidine on intestinal ischemia‑reperfusion injury. Experimental and Therapeutic Medicine, 10, 647-652. https://doi.org/10.3892/etm.2015.2561
MLA
Sun, Y., Gao, Q., Wu, N., Li, S., Yao, J., Fan, W."Protective effects of dexmedetomidine on intestinal ischemia‑reperfusion injury". Experimental and Therapeutic Medicine 10.2 (2015): 647-652.
Chicago
Sun, Y., Gao, Q., Wu, N., Li, S., Yao, J., Fan, W."Protective effects of dexmedetomidine on intestinal ischemia‑reperfusion injury". Experimental and Therapeutic Medicine 10, no. 2 (2015): 647-652. https://doi.org/10.3892/etm.2015.2561
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Y, Gao Q, Wu N, Li SD, Yao JX and Fan WJ: Protective effects of dexmedetomidine on intestinal ischemia‑reperfusion injury. Exp Ther Med 10: 647-652, 2015.
APA
Sun, Y., Gao, Q., Wu, N., Li, S., Yao, J., & Fan, W. (2015). Protective effects of dexmedetomidine on intestinal ischemia‑reperfusion injury. Experimental and Therapeutic Medicine, 10, 647-652. https://doi.org/10.3892/etm.2015.2561
MLA
Sun, Y., Gao, Q., Wu, N., Li, S., Yao, J., Fan, W."Protective effects of dexmedetomidine on intestinal ischemia‑reperfusion injury". Experimental and Therapeutic Medicine 10.2 (2015): 647-652.
Chicago
Sun, Y., Gao, Q., Wu, N., Li, S., Yao, J., Fan, W."Protective effects of dexmedetomidine on intestinal ischemia‑reperfusion injury". Experimental and Therapeutic Medicine 10, no. 2 (2015): 647-652. https://doi.org/10.3892/etm.2015.2561
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