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Article

Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines

  • Authors:
    • Xiao‑Wei Wang
    • Wei‑Dong Li
    • Jin‑Rong Xia
    • Zhan Li
    • Xiao‑Gang Cai
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Gastroenterology, Langxi Chinese Medicine Hospital, Langxi, Anhui 242100, P.R. China
  • Pages: 584-590
    |
    Published online on: June 11, 2015
       https://doi.org/10.3892/etm.2015.2569
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Abstract

The aim of the present study was to investigate the effect of receptor for advanced glycation end products (RAGE)‑specific small interfering (si)RNA on the generation of proinflammatory cytokines in primary rat hepatic stellate cells (HSCs) and hepatic fibrotic (HF) rats. The RAGE‑specific siRNA expression vector pAKD‑GR126 was constructed, and then transfected into primary rat HSCs. Reverse transcription‑quantitative polymerase chain reaction and western blot analyses were conducted to determine the mRNA and protein expression levels, respectively, of RAGE, tumor necrosis factor (TNF)‑α and interleukin (IL)‑6 in the primary HSCs. In addition, a CCl4‑induced Sprague Dawley (SD) rat model of hepatic fibrosis was established, and pAKD‑GR126 was injected into the SD rats via the tail vein. Serum TNF‑α and IL‑6 concentrations were determined using radioimmunoassay. The mRNA and protein expression levels of RAGE (mRNA, F=7.791; protein, F=36.513), TNF‑α (mRNA, F=474.568; protein, F=123.500) and IL‑6 (mRNA, F=203.463; protein, F=320.555) in the pAKD‑GR126‑transfected primary HSCs were significantly reduced compared with those in the control and pAKD‑NC groups (P<0.05). Serum TNF‑α and IL‑6 levels in the low‑, medium‑ and high‑dose pAKD‑GR126 treatment groups were reduced compared with those in the fibrotic model group (TNF‑α, F=416.397; IL‑6, F=1,716.659; P<0.05). In summary, the RAGE‑specific siRNA was able to effectively suppress the generation of the proinflammatory cytokines TNF‑α and IL‑6 in primary rat HSCs and HF rats.
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1 

Mansoor S, Yerian L, Kohli R, Xanthakos S, Angulo P, Ling S, Lopez R, Christine CK, Feldstein AE and Alkhouri N: The evaluation of hepatic fibrosis scores in children with nonalcoholic fatty liver disease. Dig Dis Sci. 60:1440–1447. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Roy S, Benz F, Luedde T and Roderburg C: The role of miRNAs in the regulation of inflammatory processes during hepatofibrogenesis. Hepatobiliary Surg Nutr. 4:24–33. 2015.PubMed/NCBI

3 

Hegab Z, Gibbons S, Neyses L and Mamas MA: Role of advanced glycation end products in cardiovascular disease. World J Cardiol. 4:90–102. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Barlovic DP, Soro-Paavonen A and Jandeleit-Dahm KA: RAGE biology, atherosclerosis and diabetes. Clin Sci (Lond). 121:43–55. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Lee UE and Friedman SL: Mechanisms of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol. 25:195–206. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Lohwasser C, Neureiter D, Popov Y, Bauer M and Schuppan D: Role of the receptor for advanced glycation end products in hepatic fibrosis. World J Gastroenterol. 15:5789–5798. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Guimarães EL, Empsen C, Geerts A and van Grunsven LA: Advanced glycation end products induce production of reactive oxygen species via the activation of NADPH oxidase in murine hepatic stellate cells. J Hepatol. 52:389–397. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Xia JR, Liu NF and Zhu NX: Specific siRNA targeting the receptor for advanced glycation end products inhibits experimental hepatic fibrosis in rats. Int J Mol Sci. 9:638–661. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Lin J, Tang Y, Kang Q, Feng Y and Chen A: Curcumin inhibits gene expression of receptor for advanced glycation end-products (RAGE) in hepatic stellate cells in vitro by elevating PPARγ activity and attenuating oxidative stress. Br J Pharmacol. 166:2212–2227. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Goodwin M, Herath C, Jia Z, Leung C, Coughlan MT, Forbes J and Angus P: Advanced glycation end products augment experimental hepatic fibrosis. J Gastroenterol Hepatol. 28:369–376. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Hsu WH, Lee BH, Hsu YW and Pan TM: Peroxisome proliferator-activated receptor-γ activators monascin and rosiglitazone attenuate carboxymethyllysine-induced fibrosis in hepatic stellate cells through regulating the oxidative stress pathway but independent of the receptor for advanced glycation end products signaling. J Agric Food Chem. 61:6873–6879. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Scheuer PJ: Classification of chronic viral hepatitis: A need for reassessment. J Hepatol. 13:372–374. 1991. View Article : Google Scholar : PubMed/NCBI

13 

Hyogo H and Yamagishi S: Advanced glycation end products (AGEs) and their involvement in liver disease. Curr Pharm Des. 14:969–972. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Pusterla T, Nèmeth J, Stein I, Wiechert L, Knigin D, Marhenke S, Longerich T, Kumar V, Arnold B, Vogel A, et al: Receptor for advanced glycation endproducts (RAGE) is a key regulator of oval cell activation and inflammation-associated liver carcinogenesis in mice. Hepatology. 58:363–373. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Watson AM, Gray SP, Jiaze L, Soro-Paavonen A, Wong B, Cooper ME, Bierhaus A, Pickering R, Tikellis C, Tsorotes D, et al: Alagebrium reduces glomerular fibrogenesis and inflammation beyond preventing RAGE activation in diabetic apolipoprotein E knockout mice. Diabetes. 61:2105–2113. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Fehrenbach H, Weiskirchen R, Kasper M and Gressner AM: Up-regulated expression of the receptor for advanced glycation end products in cultured rat hepatic stellate cells during transdifferentiation to myofibroblasts. Hepatology. 34:943–952. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Zhang SC, Zheng YH, Yu PP, Min TH, Yu FX, Ye C, Xie YK and Zhang QY: Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6. World J Gastroenterol. 18:3696–3704. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Lee BH, Hsu WH, Hsu YW and Pan TM: Suppression of dimerumic acid on hepatic fibrosis caused from carboxymethyl-lysine (CML) by attenuating oxidative stress depends on Nrf2 activation in hepatic stellate cells (HSCs). Food Chem Toxicol. 62:413–419. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Tang Y and Chen A: Curcumin eliminates the effect of advanced glycation end-products (AGEs) on the divergent regulation of gene expression of receptors of AGEs by interrupting leptin signaling. Lab Invest. 94:503–516. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Klironomos S, Notas G, Sfakianaki O, Kiagiadaki F, Xidakis C and Kouroumalis E: Octreotide modulates the effects on fibrosis of TNF-α, TGF-β and PDGF in activated rat hepatic stellate cells. Regul Pept. 188:5–12. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Ming-Ju H, Yih-Shou H, Tzy-Yen C and Hui-Ling C: Hepatitis C virus E2 protein induce reactive oxygen species (ROS)-related fibrogenesis in the HSC-T6 hepatic stellate cell line. J Cell Biochem. 112:233–243. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Radwan MI, Pasha HF, Mohamed RH, Hussien HI and El-Khshab MN: Influence of transforming growth factor-β1 and tumor necrosis factor-α genes polymorphisms on the development of cirrhosis and hepatocellular carcinoma in chronic hepatitis C patients. Cytokine. 60:271–276. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Yang YY, Liu RS, Lee PC, et al: Anti-VEGFR agents ameliorate hepatic venous dysregulation/microcirculatory dysfunction, splanchnic venous pooling and ascites of NASH-cirrhotic rat. Liver Int. 34:521–534. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang XW, Li WD, Xia JR, Li Z and Cai XG: Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines. Exp Ther Med 10: 584-590, 2015.
APA
Wang, X., Li, W., Xia, J., Li, Z., & Cai, X. (2015). Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines. Experimental and Therapeutic Medicine, 10, 584-590. https://doi.org/10.3892/etm.2015.2569
MLA
Wang, X., Li, W., Xia, J., Li, Z., Cai, X."Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines". Experimental and Therapeutic Medicine 10.2 (2015): 584-590.
Chicago
Wang, X., Li, W., Xia, J., Li, Z., Cai, X."Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines". Experimental and Therapeutic Medicine 10, no. 2 (2015): 584-590. https://doi.org/10.3892/etm.2015.2569
Copy and paste a formatted citation
x
Spandidos Publications style
Wang XW, Li WD, Xia JR, Li Z and Cai XG: Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines. Exp Ther Med 10: 584-590, 2015.
APA
Wang, X., Li, W., Xia, J., Li, Z., & Cai, X. (2015). Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines. Experimental and Therapeutic Medicine, 10, 584-590. https://doi.org/10.3892/etm.2015.2569
MLA
Wang, X., Li, W., Xia, J., Li, Z., Cai, X."Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines". Experimental and Therapeutic Medicine 10.2 (2015): 584-590.
Chicago
Wang, X., Li, W., Xia, J., Li, Z., Cai, X."Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines". Experimental and Therapeutic Medicine 10, no. 2 (2015): 584-590. https://doi.org/10.3892/etm.2015.2569
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