Role of (-)-epigallocatechin-3-gallate in the osteogenic differentiation of human bone marrow mesenchymal stem cells: An enhancer or an inducer? Corrigendum in /10.3892/etm.2021.9725

Jin,Pan; Li,Muyan; Xu,Guojie; Zhang,Kun; Zheng,Li; Zhao,Jinmin

doi:10.3892/etm.2015.2579

Role of (-)-epigallocatechin-3-gallate in the osteogenic differentiation of human bone marrow mesenchymal stem cells: An enhancer or an inducer?

Corrigendum in: /10.3892/etm.2021.9725

Authors:
- Pan Jin
- Muyan Li
- Guojie Xu
- Kun Zhang
- Li Zheng
- Jinmin Zhao
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Affiliations: Department of Orthopedic Surgery, The First Hospital Affiliated to Henan University, Kaifeng, Henan 475001, P.R. China, Medical and Scientific Research Center, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Published online on: June 16, 2015 https://doi.org/10.3892/etm.2015.2579
Pages: 828-834

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Abstract

Epidemiological investigations have revealed that the consumption of green tea, which is a rich source of (-)-epigallocatechin-3-gallate (EGCG), is associated with a reduced risk of osteoporosis. A number of in vitro and in vivo studies have also demonstrated that EGCG exerts a significant positive effect on osteogenesis; however, the single effect of EGCG on osteogenic differentiation has been seldom studied. EGCG was hypothesized to function as an enhancer or an inducer. In the present study, the effect of EGCG on the osteogenic differentiation of primary human bone marrow mesenchymal stem cells (hBMSCs), without other additives, was investigated. Three groups of stem cells were analyzed, which included a negative control group (hBMSCs cultured with culture medium only), an experimental group (cells treated with culture medium containing 2.5, 5 and 10 µM EGCG), and a positive control group (cells cultured with osteogenesis‑induced culture medium). After 3, 7, 14 and 21 days, cell proliferation, alkaline phosphatase (ALP) activity and the expression of associated osteogenic genes were analyzed. The results revealed that ALP activity and the expression of associated osteogenic genes, with the exception of bone morphogenetic protein 2 (BMP2), were not affected by EGCG treatment alone. These results indicated that EGCG itself had little effect on the osteogenic differentiation of MSCs; however, EGCG was able to enhance osteogenesis in the presence of osteoinductive agents through the upregulation of BMP2 expression. Additionally, EGCG was shown to promote cell growth, demonstrating its safety as a therapeutic agent. Therefore, the present study indicated that treatment with EGCG was dependent on other osteogenic inducers.

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1	Hoover PA, Webber CE, Beaumont LF and Blake JM: Postmenopausal bone mineral density: Relationship to calcium intake, calcium absorption, residual estrogen, body composition and physical activity. Can J Physiol Pharmacol. 74:911–917. 1996. View Article : Google Scholar : PubMed/NCBI
2	Hegarty VM, May HM and Khaw KT: Tea drinking and bone mineral density in older women. Am J Clin Nutr. 71:1003–1007. 2000.PubMed/NCBI
3	Wu CH, Yang YC, Yao WJ, et al: Epidemiological evidence of increased bone mineral density in habitual tea drinkers. Arch Intern Med. 162:1001–1006. 2002. View Article : Google Scholar : PubMed/NCBI
4	Chen Z, Pettinger MB, Ritenbaugh C, et al: Habitual tea consumption and risk of osteoporosis: A prospective study in the women's health initiative observational cohort. Am J Epidemiol. 158:772–781. 2003. View Article : Google Scholar : PubMed/NCBI
5	Muraki S, Yamamoto S, Ishibashi H, et al: Diet and lifestyle associated with increased bone mineral density: Cross-sectional study of Japanese elderly women at an osteoporosis outpatient clinic. J Orthop Sci. 12:317–320. 2007. View Article : Google Scholar : PubMed/NCBI
6	Devine A, Hodgson JM, Dick IM and Prince RL: Tea drinking is associated with benefits on bone density in older women. Am J Clin Nutr. 86:1243–1247. 2007.PubMed/NCBI
7	Johnell O, Gullberg B, Kanis JA, et al: Risk factors for hip fracture in European women: The MEDOS study. Mediterranean Osteoporosis Study. J Bone Miner Res. 10:1802–1815. 1995. View Article : Google Scholar : PubMed/NCBI
8	Kanis J, Johnell O, Gullberg B, et al: Risk factors for hip fracture in men from southern Europe: The MEDOS study. Mediterranean Osteoporosis Study. Osteoporos Int. 9:45–54. 1999. View Article : Google Scholar : PubMed/NCBI
9	Harbowy ME and Balentine DA: Tea chemistry. Crit Rev Plant Sci. 16:415–480. 1997. View Article : Google Scholar
10	Chen CH, Ho ML, Chang JK, et al: Green tea catechins enhance the expression of osteoprotegerin (OPG) in pluripotent stem cells. J Orthop Surg Taiwan. 20:178–183. 2003.
11	Dulloo AG, Duret C, Rohrer D, et al: Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation in humans. Am J Clin Nutr. 70:1040–1045. 1999.PubMed/NCBI
12	Stagg GV and Millin DJ: The nutritional and therapeutic value of tea - a review. J Sci Food Agric. 26:1439–1459. 1975. View Article : Google Scholar
13	Shen CL, Wang P, Guerrieri J, et al: Protective effect of green tea polyphenols on bone loss in middle-aged female rats. Osteoporos Int. 19:979–990. 2008. View Article : Google Scholar : PubMed/NCBI
14	Shen CL, Yeh JK, Stoecker BJ, et al: Green tea polyphenols mitigate deterioration of bone microarchitecture in middle-aged female rats. Bone. 44:684–690. 2009. View Article : Google Scholar : PubMed/NCBI
15	Shen CL, Yeh JK, Cao JJ, et al: Green tea polyphenols mitigate bone loss of female rats in a chronic inflammation-induced bone loss model. J Nutr Biochem. 21:968–974. 2010. View Article : Google Scholar : PubMed/NCBI
16	Shen CL, Yeh JK, Samathanam C, et al: Green tea polyphenols attenuate deterioration of bone microarchitecture in female rats with systemic chronic inflammation. Osteoporos Int. 22:327–337. 2011. View Article : Google Scholar : PubMed/NCBI
17	Shen CL, Cao JJ, Dagda RY, et al: Supplementation with green tea polyphenols improves bone microstructure and quality in aged, orchidectomized rats. Calcif Tissue Int. 88:455–463. 2011. View Article : Google Scholar : PubMed/NCBI
18	Rodriguez R, Kondo H, Nyan M, et al: Implantation of green tea catechin α-tricalcium phosphate combination enhances bone repair in rat skull defects. J Biomed Mater Res B Appl Biomater. 98:263–271. 2011. View Article : Google Scholar : PubMed/NCBI
19	Vali B, Rao LG and El-Sohemy A: Epigallocatechin-3-gallate increases the formation of mineralized bone nodules by human osteoblast-like cells. J Nutr Biochem. 18:341–347. 2007. View Article : Google Scholar : PubMed/NCBI
20	Chen CH, Ho ML, Chang JK, et al: Green tea catechin enhances osteogenesis in a bone marrow mesenchymal stem cell line. Osteoporos Int. 16:2039–2045. 2005. View Article : Google Scholar : PubMed/NCBI
21	Nakagawa H, Wachi M, Woo JT, et al: Fenton reaction is primarily involved in a mechanism of (-)-epigallocatechin-3-gallate to induce osteoclastic cell death. Biochem Bioph Res Commun. 292:94–101. 2002. View Article : Google Scholar
22	Yun JH, Pang EK, Kim CS, et al: Inhibitory effects of green tea polyphenol (-)-epigallocatechin gallate on the expression of matrix metalloproteinase-9 and on the formation of osteoclasts. J Periodontal Res. 39:300–307. 2004. View Article : Google Scholar : PubMed/NCBI
23	Jin P, Wu H, Xu G, et al: Epigallocatechin-3-gallate (EGCG) as a pro-osteogenic agent to enhance osteogenic differentiation of mesenchymal stem cells from human bone marrow: An in vitro study. Cell Tissue Res. 356:381–390. 2014. View Article : Google Scholar : PubMed/NCBI
24	Na K, Sun BK, Woo DG, et al: Osteogenic differentiation of rabbit mesenchymal stem cells in thermo-reversible hydrogel constructs containing hydroxyapatite and bone morphogenic protein-2 (BMP-2). Biomaterials. 28:2631–2637. 2007. View Article : Google Scholar : PubMed/NCBI
25	Harris SE, Bonewald LF, Harris MA, et al: Effects of transforming growth factor beta on bone nodule formation and expression of bone morphogenetic protein 2, osteocalcin, osteopontin, alkaline phosphatase and type I collagen mRNA in long-term cultures of fetal rat calvarial osteoblasts. J Bone Miner Res. 9:855–863. 1994. View Article : Google Scholar : PubMed/NCBI
26	Hogan BL: Bone morphogenetic proteins: Multifunctional regulators of vertebrate development. Genes Dev. 10:1580–1594. 1996. View Article : Google Scholar : PubMed/NCBI
27	Reddi AH: Bone and cartilage differentiation. Curr Opin Genet Dev. 4:737–744. 1994. View Article : Google Scholar : PubMed/NCBI
28	Wozney JM, Rosen V, Celeste AJ, et al: Novel regulators of bone formation: Molecular clones and activities. Science. 242:1528–1534. 1988. View Article : Google Scholar : PubMed/NCBI
29	Wozney JM: The bone morphogenetic protein family and osteogenesis. Mol Reprod Dev. 32:160–167. 1992. View Article : Google Scholar : PubMed/NCBI
30	Yamaguchi A, Katagiri T, Ikeda T, et al: Recombinant human bone morphogenetic protein-2 stimulates osteoblastic maturation and inhibits myogenic differentiation in vitro. J Cell Biol. 113:681–687. 1991. View Article : Google Scholar : PubMed/NCBI
31	Harborne JB and Williams CA: Advances in flavonoid research since 1992. Phytochemistry. 55:481–504. 2000. View Article : Google Scholar : PubMed/NCBI
32	Kamon M, Zhao R and Sakamoto K: Green tea polyphenol (-)-epigallocatechin gallate suppressed the differentiation of murine osteoblastic MC3T3-E1 cells. Cell Biol Int. 34:109–116. 2009.PubMed/NCBI
33	Yang CH, Lin CY, Yang JH, et al: Supplementary catechins attenuate cooking-oil-fumes-induced oxidative stress in rat lung. Chin J Physiol. 52:151–159. 2009. View Article : Google Scholar : PubMed/NCBI
34	Yagi H, Tan J and Tuan RS: Polyphenols suppress hydrogen peroxide-induced oxidative stress in human bone-marrow derived mesenchymal stem cells. J Cell Biochem. 114:1163–1173. 2013. View Article : Google Scholar : PubMed/NCBI
35	Hanai J, Chen LF, Kanno T, et al: Interaction and functional cooperation of PEBP2/CBF with Smads. Synergistic induction of the immunoglobulin germline C-alpha promoter. J Biol Chem. 274:31577–31582. 1999. View Article : Google Scholar : PubMed/NCBI
36	Javed A, Barnes GL, Jasanya BO, et al: Runt homology domain transcription factors (Runx, Cbfa and AML) mediate repression of the bone sialoprotein promoter: Evidence for promoter context-dependent activity of Cbfa proteins. Mol Cell Biol. 21:2891–2905. 2001. View Article : Google Scholar : PubMed/NCBI
37	Nishimura R, Hata K, Harris SE, et al: Core-binding factor alpha 1 (Cbfa1) induces osteoblastic differentiation of C2C12 cells without interactions with Smad1 and Smad5. Bone. 31:303–312. 2002. View Article : Google Scholar : PubMed/NCBI